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A new animal model containing human SCARB2 and lacking stat-1 is highly susceptible to EV71
Enterovirus 71 (EV71) is a major threat to children worldwide. Children infected with EV71 could develop subclinical infection and hand-foot-and -mouth disease (HFMD). In severe cases, patients could develop encephalitis, paralysis, pulmonary edema, and death. A more user-friendly and robust animal...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4976353/ https://www.ncbi.nlm.nih.gov/pubmed/27499235 http://dx.doi.org/10.1038/srep31151 |
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author | Liou, An-Ting Wu, Szu-Yao Liao, Chun-Che Chang, Ya-Shu Chang, Chih-Shin Shih, Chiaho |
author_facet | Liou, An-Ting Wu, Szu-Yao Liao, Chun-Che Chang, Ya-Shu Chang, Chih-Shin Shih, Chiaho |
author_sort | Liou, An-Ting |
collection | PubMed |
description | Enterovirus 71 (EV71) is a major threat to children worldwide. Children infected with EV71 could develop subclinical infection and hand-foot-and -mouth disease (HFMD). In severe cases, patients could develop encephalitis, paralysis, pulmonary edema, and death. A more user-friendly and robust animal model is essential to investigating EV71 pathogenesis. Here, we established a hybrid (hSCARB2(+/+)/stat-1(−/−)) mouse strain from crossbreeding SCARB2 transgenic and stat-1 KO mice, and compared the susceptibilities to EV71 infection and pathogenesis between parental and hybrid mice. Virus-encoded VP1 protein can be detected in the streaking nerve fibers in brain and spinal cord. This hybrid mouse strain at 2-week-old age can still be infected with different genotypes of EV71 at 1000-fold lower titer via an ip route. Infected hybrid mice developed earlier onset of CNS disease, paralysis, and death at a higher incidence. These advantages of this novel model meet the urgent need from the scientific community in basic and preclinical research in therapeutics and pathogenesis. |
format | Online Article Text |
id | pubmed-4976353 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49763532016-08-22 A new animal model containing human SCARB2 and lacking stat-1 is highly susceptible to EV71 Liou, An-Ting Wu, Szu-Yao Liao, Chun-Che Chang, Ya-Shu Chang, Chih-Shin Shih, Chiaho Sci Rep Article Enterovirus 71 (EV71) is a major threat to children worldwide. Children infected with EV71 could develop subclinical infection and hand-foot-and -mouth disease (HFMD). In severe cases, patients could develop encephalitis, paralysis, pulmonary edema, and death. A more user-friendly and robust animal model is essential to investigating EV71 pathogenesis. Here, we established a hybrid (hSCARB2(+/+)/stat-1(−/−)) mouse strain from crossbreeding SCARB2 transgenic and stat-1 KO mice, and compared the susceptibilities to EV71 infection and pathogenesis between parental and hybrid mice. Virus-encoded VP1 protein can be detected in the streaking nerve fibers in brain and spinal cord. This hybrid mouse strain at 2-week-old age can still be infected with different genotypes of EV71 at 1000-fold lower titer via an ip route. Infected hybrid mice developed earlier onset of CNS disease, paralysis, and death at a higher incidence. These advantages of this novel model meet the urgent need from the scientific community in basic and preclinical research in therapeutics and pathogenesis. Nature Publishing Group 2016-08-08 /pmc/articles/PMC4976353/ /pubmed/27499235 http://dx.doi.org/10.1038/srep31151 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Liou, An-Ting Wu, Szu-Yao Liao, Chun-Che Chang, Ya-Shu Chang, Chih-Shin Shih, Chiaho A new animal model containing human SCARB2 and lacking stat-1 is highly susceptible to EV71 |
title | A new animal model containing human SCARB2 and lacking stat-1 is highly susceptible to EV71 |
title_full | A new animal model containing human SCARB2 and lacking stat-1 is highly susceptible to EV71 |
title_fullStr | A new animal model containing human SCARB2 and lacking stat-1 is highly susceptible to EV71 |
title_full_unstemmed | A new animal model containing human SCARB2 and lacking stat-1 is highly susceptible to EV71 |
title_short | A new animal model containing human SCARB2 and lacking stat-1 is highly susceptible to EV71 |
title_sort | new animal model containing human scarb2 and lacking stat-1 is highly susceptible to ev71 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4976353/ https://www.ncbi.nlm.nih.gov/pubmed/27499235 http://dx.doi.org/10.1038/srep31151 |
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