Cargando…
Real-time optical diagnosis of gastric cancer with serosal invasion using multiphoton imaging
A real-time optical biopsy, which could determine tissue histopathology, would be of extraordinary benefit to staging laparoscopy for gastric cancer with serosal invasion (T4) that requires downstage treatment. We investigated the feasibility of using multiphoton imaging to perform a real-time optic...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4976383/ https://www.ncbi.nlm.nih.gov/pubmed/27499365 http://dx.doi.org/10.1038/srep31004 |
_version_ | 1782446866406834176 |
---|---|
author | Yan, Jun Zheng, Yu Zheng, Xiaoling Liu, Zhangyuanzhu Liu, Wenju Chen, Dexin Dong, Xiaoyu Li, Kai Liu, Xiumin Chen, Gang Lu, Jianping Chen, Jianxin Zhuo, Shuangmu Li, Guoxin |
author_facet | Yan, Jun Zheng, Yu Zheng, Xiaoling Liu, Zhangyuanzhu Liu, Wenju Chen, Dexin Dong, Xiaoyu Li, Kai Liu, Xiumin Chen, Gang Lu, Jianping Chen, Jianxin Zhuo, Shuangmu Li, Guoxin |
author_sort | Yan, Jun |
collection | PubMed |
description | A real-time optical biopsy, which could determine tissue histopathology, would be of extraordinary benefit to staging laparoscopy for gastric cancer with serosal invasion (T4) that requires downstage treatment. We investigated the feasibility of using multiphoton imaging to perform a real-time optical diagnosis of gastric cancer with or without serosal invasion. First, a pilot study was performed to establish the optical diagnostic features of gastric cancer with or without serosal invasion using multiphoton imaging compared with hematoxylin-eosin staining and Masson’s trichrome staining. Second, a blinded study was performed to compare the diagnostic sensitivity, specificity, and accuracy of multiphoton imaging and endoscopic ultrasonography (EUS) for T4 gastric cancer. In the pilot study, multiphoton imaging revealed collagen loss and degradation and cellular and nuclear pleomorphism in gastric cancer with serosal invasion. The collagen content in gastric cancer with or without serosal invasion was 0.36 ± 0.18 and 0.79 ± 0.16 (p < 0.001), respectively. In the blinded study, the sensitivity, specificity, and accuracy of EUS and multiphoton imaging for T4 gastric cancer were 70% and 90% (p = 0.029), 66.67% and 96.67% (p = 0.003), and 68.33% and 93.33% (p = 0.001), respectively. It is feasible to use multiphoton imaging to make a real-time optical diagnosis of gastric cancer with or without serosal invasion. |
format | Online Article Text |
id | pubmed-4976383 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49763832016-08-22 Real-time optical diagnosis of gastric cancer with serosal invasion using multiphoton imaging Yan, Jun Zheng, Yu Zheng, Xiaoling Liu, Zhangyuanzhu Liu, Wenju Chen, Dexin Dong, Xiaoyu Li, Kai Liu, Xiumin Chen, Gang Lu, Jianping Chen, Jianxin Zhuo, Shuangmu Li, Guoxin Sci Rep Article A real-time optical biopsy, which could determine tissue histopathology, would be of extraordinary benefit to staging laparoscopy for gastric cancer with serosal invasion (T4) that requires downstage treatment. We investigated the feasibility of using multiphoton imaging to perform a real-time optical diagnosis of gastric cancer with or without serosal invasion. First, a pilot study was performed to establish the optical diagnostic features of gastric cancer with or without serosal invasion using multiphoton imaging compared with hematoxylin-eosin staining and Masson’s trichrome staining. Second, a blinded study was performed to compare the diagnostic sensitivity, specificity, and accuracy of multiphoton imaging and endoscopic ultrasonography (EUS) for T4 gastric cancer. In the pilot study, multiphoton imaging revealed collagen loss and degradation and cellular and nuclear pleomorphism in gastric cancer with serosal invasion. The collagen content in gastric cancer with or without serosal invasion was 0.36 ± 0.18 and 0.79 ± 0.16 (p < 0.001), respectively. In the blinded study, the sensitivity, specificity, and accuracy of EUS and multiphoton imaging for T4 gastric cancer were 70% and 90% (p = 0.029), 66.67% and 96.67% (p = 0.003), and 68.33% and 93.33% (p = 0.001), respectively. It is feasible to use multiphoton imaging to make a real-time optical diagnosis of gastric cancer with or without serosal invasion. Nature Publishing Group 2016-08-08 /pmc/articles/PMC4976383/ /pubmed/27499365 http://dx.doi.org/10.1038/srep31004 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Yan, Jun Zheng, Yu Zheng, Xiaoling Liu, Zhangyuanzhu Liu, Wenju Chen, Dexin Dong, Xiaoyu Li, Kai Liu, Xiumin Chen, Gang Lu, Jianping Chen, Jianxin Zhuo, Shuangmu Li, Guoxin Real-time optical diagnosis of gastric cancer with serosal invasion using multiphoton imaging |
title | Real-time optical diagnosis of gastric cancer with serosal invasion using multiphoton imaging |
title_full | Real-time optical diagnosis of gastric cancer with serosal invasion using multiphoton imaging |
title_fullStr | Real-time optical diagnosis of gastric cancer with serosal invasion using multiphoton imaging |
title_full_unstemmed | Real-time optical diagnosis of gastric cancer with serosal invasion using multiphoton imaging |
title_short | Real-time optical diagnosis of gastric cancer with serosal invasion using multiphoton imaging |
title_sort | real-time optical diagnosis of gastric cancer with serosal invasion using multiphoton imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4976383/ https://www.ncbi.nlm.nih.gov/pubmed/27499365 http://dx.doi.org/10.1038/srep31004 |
work_keys_str_mv | AT yanjun realtimeopticaldiagnosisofgastriccancerwithserosalinvasionusingmultiphotonimaging AT zhengyu realtimeopticaldiagnosisofgastriccancerwithserosalinvasionusingmultiphotonimaging AT zhengxiaoling realtimeopticaldiagnosisofgastriccancerwithserosalinvasionusingmultiphotonimaging AT liuzhangyuanzhu realtimeopticaldiagnosisofgastriccancerwithserosalinvasionusingmultiphotonimaging AT liuwenju realtimeopticaldiagnosisofgastriccancerwithserosalinvasionusingmultiphotonimaging AT chendexin realtimeopticaldiagnosisofgastriccancerwithserosalinvasionusingmultiphotonimaging AT dongxiaoyu realtimeopticaldiagnosisofgastriccancerwithserosalinvasionusingmultiphotonimaging AT likai realtimeopticaldiagnosisofgastriccancerwithserosalinvasionusingmultiphotonimaging AT liuxiumin realtimeopticaldiagnosisofgastriccancerwithserosalinvasionusingmultiphotonimaging AT chengang realtimeopticaldiagnosisofgastriccancerwithserosalinvasionusingmultiphotonimaging AT lujianping realtimeopticaldiagnosisofgastriccancerwithserosalinvasionusingmultiphotonimaging AT chenjianxin realtimeopticaldiagnosisofgastriccancerwithserosalinvasionusingmultiphotonimaging AT zhuoshuangmu realtimeopticaldiagnosisofgastriccancerwithserosalinvasionusingmultiphotonimaging AT liguoxin realtimeopticaldiagnosisofgastriccancerwithserosalinvasionusingmultiphotonimaging |