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Stereological Analysis of Liver Biopsy Histology Sections as a Reference Standard for Validating Non-Invasive Liver Fat Fraction Measurements by MRI

BACKGROUND AND AIMS: Validation of non-invasive methods of liver fat quantification requires a reference standard. However, using standard histopathology assessment of liver biopsies is problematical because of poor repeatability. We aimed to assess a stereological method of measuring volumetric liv...

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Autores principales: St. Pierre, Tim G., House, Michael J., Bangma, Sander J., Pang, Wenjie, Bathgate, Andrew, Gan, Eng K., Ayonrinde, Oyekoya T., Bhathal, Prithi S., Clouston, Andrew, Olynyk, John K., Adams, Leon A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4976876/
https://www.ncbi.nlm.nih.gov/pubmed/27501242
http://dx.doi.org/10.1371/journal.pone.0160789
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author St. Pierre, Tim G.
House, Michael J.
Bangma, Sander J.
Pang, Wenjie
Bathgate, Andrew
Gan, Eng K.
Ayonrinde, Oyekoya T.
Bhathal, Prithi S.
Clouston, Andrew
Olynyk, John K.
Adams, Leon A.
author_facet St. Pierre, Tim G.
House, Michael J.
Bangma, Sander J.
Pang, Wenjie
Bathgate, Andrew
Gan, Eng K.
Ayonrinde, Oyekoya T.
Bhathal, Prithi S.
Clouston, Andrew
Olynyk, John K.
Adams, Leon A.
author_sort St. Pierre, Tim G.
collection PubMed
description BACKGROUND AND AIMS: Validation of non-invasive methods of liver fat quantification requires a reference standard. However, using standard histopathology assessment of liver biopsies is problematical because of poor repeatability. We aimed to assess a stereological method of measuring volumetric liver fat fraction (VLFF) in liver biopsies and to use the method to validate a magnetic resonance imaging method for measurement of VLFF. METHODS: VLFFs were measured in 59 subjects (1) by three independent analysts using a stereological point counting technique combined with the Delesse principle on liver biopsy histological sections and (2) by three independent analysts using the HepaFat-Scan(®) technique on magnetic resonance images of the liver. Bland Altman statistics and intraclass correlation (IC) were used to assess the repeatability of each method and the bias between the methods of liver fat fraction measurement. RESULTS: Inter-analyst repeatability coefficients for the stereology and HepaFat-Scan(®) methods were 8.2 (95% CI 7.7–8.8)% and 2.4 (95% CI 2.2–2.5)% VLFF respectively. IC coefficients were 0.86 (95% CI 0.69–0.93) and 0.990 (95% CI 0.985–0.994) respectively. Small biases (≤3.4%) were observable between two pairs of analysts using stereology while no significant biases were observable between any of the three pairs of analysts using HepaFat-Scan(®). A bias of 1.4±0.5% VLFF was observed between the HepaFat-Scan(®) method and the stereological method. CONCLUSIONS: Repeatability of the stereological method is superior to the previously reported performance of assessment of hepatic steatosis by histopathologists and is a suitable reference standard for validating non-invasive methods of measurement of VLFF.
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spelling pubmed-49768762016-08-25 Stereological Analysis of Liver Biopsy Histology Sections as a Reference Standard for Validating Non-Invasive Liver Fat Fraction Measurements by MRI St. Pierre, Tim G. House, Michael J. Bangma, Sander J. Pang, Wenjie Bathgate, Andrew Gan, Eng K. Ayonrinde, Oyekoya T. Bhathal, Prithi S. Clouston, Andrew Olynyk, John K. Adams, Leon A. PLoS One Research Article BACKGROUND AND AIMS: Validation of non-invasive methods of liver fat quantification requires a reference standard. However, using standard histopathology assessment of liver biopsies is problematical because of poor repeatability. We aimed to assess a stereological method of measuring volumetric liver fat fraction (VLFF) in liver biopsies and to use the method to validate a magnetic resonance imaging method for measurement of VLFF. METHODS: VLFFs were measured in 59 subjects (1) by three independent analysts using a stereological point counting technique combined with the Delesse principle on liver biopsy histological sections and (2) by three independent analysts using the HepaFat-Scan(®) technique on magnetic resonance images of the liver. Bland Altman statistics and intraclass correlation (IC) were used to assess the repeatability of each method and the bias between the methods of liver fat fraction measurement. RESULTS: Inter-analyst repeatability coefficients for the stereology and HepaFat-Scan(®) methods were 8.2 (95% CI 7.7–8.8)% and 2.4 (95% CI 2.2–2.5)% VLFF respectively. IC coefficients were 0.86 (95% CI 0.69–0.93) and 0.990 (95% CI 0.985–0.994) respectively. Small biases (≤3.4%) were observable between two pairs of analysts using stereology while no significant biases were observable between any of the three pairs of analysts using HepaFat-Scan(®). A bias of 1.4±0.5% VLFF was observed between the HepaFat-Scan(®) method and the stereological method. CONCLUSIONS: Repeatability of the stereological method is superior to the previously reported performance of assessment of hepatic steatosis by histopathologists and is a suitable reference standard for validating non-invasive methods of measurement of VLFF. Public Library of Science 2016-08-08 /pmc/articles/PMC4976876/ /pubmed/27501242 http://dx.doi.org/10.1371/journal.pone.0160789 Text en © 2016 St. Pierre et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
St. Pierre, Tim G.
House, Michael J.
Bangma, Sander J.
Pang, Wenjie
Bathgate, Andrew
Gan, Eng K.
Ayonrinde, Oyekoya T.
Bhathal, Prithi S.
Clouston, Andrew
Olynyk, John K.
Adams, Leon A.
Stereological Analysis of Liver Biopsy Histology Sections as a Reference Standard for Validating Non-Invasive Liver Fat Fraction Measurements by MRI
title Stereological Analysis of Liver Biopsy Histology Sections as a Reference Standard for Validating Non-Invasive Liver Fat Fraction Measurements by MRI
title_full Stereological Analysis of Liver Biopsy Histology Sections as a Reference Standard for Validating Non-Invasive Liver Fat Fraction Measurements by MRI
title_fullStr Stereological Analysis of Liver Biopsy Histology Sections as a Reference Standard for Validating Non-Invasive Liver Fat Fraction Measurements by MRI
title_full_unstemmed Stereological Analysis of Liver Biopsy Histology Sections as a Reference Standard for Validating Non-Invasive Liver Fat Fraction Measurements by MRI
title_short Stereological Analysis of Liver Biopsy Histology Sections as a Reference Standard for Validating Non-Invasive Liver Fat Fraction Measurements by MRI
title_sort stereological analysis of liver biopsy histology sections as a reference standard for validating non-invasive liver fat fraction measurements by mri
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4976876/
https://www.ncbi.nlm.nih.gov/pubmed/27501242
http://dx.doi.org/10.1371/journal.pone.0160789
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