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Improvement of shelf life of soymilk using immobilized protease of Oerskovia xanthineolytica NCIM 2839
Protease enzyme has lot of commercial applications, so the cost-effective production of protease using sunflower oil seed waste was carried out from Oerskovia xanthineolyitca NCIM 2839. The maximum protease production was after 24 h of incubation with 2.5 % oil seed waste concentration. O. xanthineo...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4977228/ https://www.ncbi.nlm.nih.gov/pubmed/28330233 http://dx.doi.org/10.1007/s13205-016-0479-6 |
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author | Sahoo, A. K. Gaikwad, V. S. Ranveer, R. C. Dandge, P. B. Waghmare, S. R. |
author_facet | Sahoo, A. K. Gaikwad, V. S. Ranveer, R. C. Dandge, P. B. Waghmare, S. R. |
author_sort | Sahoo, A. K. |
collection | PubMed |
description | Protease enzyme has lot of commercial applications, so the cost-effective production of protease using sunflower oil seed waste was carried out from Oerskovia xanthineolyitca NCIM 2839. The maximum protease production was after 24 h of incubation with 2.5 % oil seed waste concentration. O. xanthineolytica was found to produce two proteases—P1 and P2. The proteases were purified using 60 % cold acetone precipitation and DEAE-cellulose ion exchange chromatography. SDS-PAGE revealed molecular weight of P1 and P2 was 36 and 24 kDa, respectively. P1 and P2 were optimally active at pH 7.0 and pH 7.5 at temperature 35 and 40 °C, respectively. Analysis of hydrolyzed product of P1 and P2 by HPLC reveals that the P1 has endoprotease and P2 has exoprotease activity. The treated soy milk with immobilized proteases showed increased shelf life and removal of off flavor. |
format | Online Article Text |
id | pubmed-4977228 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-49772282016-08-09 Improvement of shelf life of soymilk using immobilized protease of Oerskovia xanthineolytica NCIM 2839 Sahoo, A. K. Gaikwad, V. S. Ranveer, R. C. Dandge, P. B. Waghmare, S. R. 3 Biotech Original Article Protease enzyme has lot of commercial applications, so the cost-effective production of protease using sunflower oil seed waste was carried out from Oerskovia xanthineolyitca NCIM 2839. The maximum protease production was after 24 h of incubation with 2.5 % oil seed waste concentration. O. xanthineolytica was found to produce two proteases—P1 and P2. The proteases were purified using 60 % cold acetone precipitation and DEAE-cellulose ion exchange chromatography. SDS-PAGE revealed molecular weight of P1 and P2 was 36 and 24 kDa, respectively. P1 and P2 were optimally active at pH 7.0 and pH 7.5 at temperature 35 and 40 °C, respectively. Analysis of hydrolyzed product of P1 and P2 by HPLC reveals that the P1 has endoprotease and P2 has exoprotease activity. The treated soy milk with immobilized proteases showed increased shelf life and removal of off flavor. Springer Berlin Heidelberg 2016-08-08 2016-12 /pmc/articles/PMC4977228/ /pubmed/28330233 http://dx.doi.org/10.1007/s13205-016-0479-6 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Sahoo, A. K. Gaikwad, V. S. Ranveer, R. C. Dandge, P. B. Waghmare, S. R. Improvement of shelf life of soymilk using immobilized protease of Oerskovia xanthineolytica NCIM 2839 |
title | Improvement of shelf life of soymilk using immobilized protease of Oerskovia xanthineolytica NCIM 2839 |
title_full | Improvement of shelf life of soymilk using immobilized protease of Oerskovia xanthineolytica NCIM 2839 |
title_fullStr | Improvement of shelf life of soymilk using immobilized protease of Oerskovia xanthineolytica NCIM 2839 |
title_full_unstemmed | Improvement of shelf life of soymilk using immobilized protease of Oerskovia xanthineolytica NCIM 2839 |
title_short | Improvement of shelf life of soymilk using immobilized protease of Oerskovia xanthineolytica NCIM 2839 |
title_sort | improvement of shelf life of soymilk using immobilized protease of oerskovia xanthineolytica ncim 2839 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4977228/ https://www.ncbi.nlm.nih.gov/pubmed/28330233 http://dx.doi.org/10.1007/s13205-016-0479-6 |
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