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A cost-effective method to enhance adenoviral transduction of primary murine osteoblasts and bone marrow stromal cells
We report here a method for the use of poly-l-lysine (PLL) to markedly improve the adenoviral transduction efficiency of primary murine osteoblasts and bone marrow stromal cells (BMSCs) in culture and in situ, which are typically difficult to transduce. We show by fluorescence microscopy and flow cy...
Autores principales: | Buo, Atum M, Williams, Mark S, Kerr, Jaclyn P, Stains, Joseph P |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4977485/ https://www.ncbi.nlm.nih.gov/pubmed/27547486 http://dx.doi.org/10.1038/boneres.2016.21 |
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