Calpains are involved in asexual and sexual development, cell wall integrity and pathogenicity of the rice blast fungus

Calpains are ubiquitous and well-conserved proteins that belong to the calcium-dependent, non-lysosomal cysteine protease family. In this study, 8 putative calpains were identified using Pfam domain analysis and BlastP searches in M. oryzae. Three single gene deletion mutants (ΔMocapn7, ΔMocapn9 and...

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Autores principales: Liu, Xiao-Hong, Ning, Guo-Ao, Huang, Lu-Yao, Zhao, Ya-Hui, Dong, Bo, Lu, Jian-Ping, Lin, Fu-Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4977516/
https://www.ncbi.nlm.nih.gov/pubmed/27502542
http://dx.doi.org/10.1038/srep31204
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author Liu, Xiao-Hong
Ning, Guo-Ao
Huang, Lu-Yao
Zhao, Ya-Hui
Dong, Bo
Lu, Jian-Ping
Lin, Fu-Cheng
author_facet Liu, Xiao-Hong
Ning, Guo-Ao
Huang, Lu-Yao
Zhao, Ya-Hui
Dong, Bo
Lu, Jian-Ping
Lin, Fu-Cheng
author_sort Liu, Xiao-Hong
collection PubMed
description Calpains are ubiquitous and well-conserved proteins that belong to the calcium-dependent, non-lysosomal cysteine protease family. In this study, 8 putative calpains were identified using Pfam domain analysis and BlastP searches in M. oryzae. Three single gene deletion mutants (ΔMocapn7, ΔMocapn9 and ΔMocapn14) and two double gene deletion mutants (ΔMocapn4ΔMocapn7 and ΔMocapn9ΔMocapn7) were obtained using the high-throughput gene knockout system. The calpain disruption mutants showed defects in colony characteristics, conidiation, sexual reproduction and cell wall integrity. The mycelia of the ΔMocapn7, ΔMocapn4ΔMocapn7 and ΔMocapn9ΔMocapn7 mutants showed reduced pathogenicity on rice and barley.
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spelling pubmed-49775162016-08-22 Calpains are involved in asexual and sexual development, cell wall integrity and pathogenicity of the rice blast fungus Liu, Xiao-Hong Ning, Guo-Ao Huang, Lu-Yao Zhao, Ya-Hui Dong, Bo Lu, Jian-Ping Lin, Fu-Cheng Sci Rep Article Calpains are ubiquitous and well-conserved proteins that belong to the calcium-dependent, non-lysosomal cysteine protease family. In this study, 8 putative calpains were identified using Pfam domain analysis and BlastP searches in M. oryzae. Three single gene deletion mutants (ΔMocapn7, ΔMocapn9 and ΔMocapn14) and two double gene deletion mutants (ΔMocapn4ΔMocapn7 and ΔMocapn9ΔMocapn7) were obtained using the high-throughput gene knockout system. The calpain disruption mutants showed defects in colony characteristics, conidiation, sexual reproduction and cell wall integrity. The mycelia of the ΔMocapn7, ΔMocapn4ΔMocapn7 and ΔMocapn9ΔMocapn7 mutants showed reduced pathogenicity on rice and barley. Nature Publishing Group 2016-08-09 /pmc/articles/PMC4977516/ /pubmed/27502542 http://dx.doi.org/10.1038/srep31204 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Liu, Xiao-Hong
Ning, Guo-Ao
Huang, Lu-Yao
Zhao, Ya-Hui
Dong, Bo
Lu, Jian-Ping
Lin, Fu-Cheng
Calpains are involved in asexual and sexual development, cell wall integrity and pathogenicity of the rice blast fungus
title Calpains are involved in asexual and sexual development, cell wall integrity and pathogenicity of the rice blast fungus
title_full Calpains are involved in asexual and sexual development, cell wall integrity and pathogenicity of the rice blast fungus
title_fullStr Calpains are involved in asexual and sexual development, cell wall integrity and pathogenicity of the rice blast fungus
title_full_unstemmed Calpains are involved in asexual and sexual development, cell wall integrity and pathogenicity of the rice blast fungus
title_short Calpains are involved in asexual and sexual development, cell wall integrity and pathogenicity of the rice blast fungus
title_sort calpains are involved in asexual and sexual development, cell wall integrity and pathogenicity of the rice blast fungus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4977516/
https://www.ncbi.nlm.nih.gov/pubmed/27502542
http://dx.doi.org/10.1038/srep31204
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