Cargando…
Fetal Bovine Serum RNA Interferes with the Cell Culture derived Extracellular RNA
Fetal bovine serum (FBS) has been used in eukaryotic cell cultures for decades. However, little attention has been paid to the biological effects associated with RNA content of FBS on cell cultures. Here, using RNA sequencing, we demonstrate that FBS contains a diverse repertoire of protein-coding a...
Autores principales: | Wei, Zhiyun, Batagov, Arsen O., Carter, David R. F., Krichevsky, Anna M. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4977539/ https://www.ncbi.nlm.nih.gov/pubmed/27503761 http://dx.doi.org/10.1038/srep31175 |
Ejemplares similares
-
Coding and noncoding landscape of extracellular RNA released by human glioma stem cells
por: Wei, Zhiyun, et al.
Publicado: (2017) -
Technical approaches to reduce interference of Fetal calf serum derived RNA in the analysis of extracellular vesicle RNA from cultured cells
por: Driedonks, Tom A. P., et al.
Publicado: (2018) -
Response to “Technical approaches to reduce interference of Fetal calf serum derived RNA in the analysis of extracellular vesicle RNA from cultured cells”
por: Lehrich, Brandon M., et al.
Publicado: (2019) -
Extracellular small non-coding RNA contaminants in fetal bovine serum and serum-free media
por: Mannerström, Bettina, et al.
Publicado: (2019) -
Fetal Bovine Serum-Derived Extracellular Vesicles Persist within Vesicle-Depleted Culture Media
por: Lehrich, Brandon M., et al.
Publicado: (2018)