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Anticonvulsant Effects of Lippia citriodora (Verbenaceae) Leaves Ethanolic Extract in Mice: Role of GABAergic System

BACKGROUND: Lippia citriodora Kunth is one of the Iranian traditional medicines for the treatment of convulsive disorders. The goal of this study is to investigate the anticonvulsant activity of the plant's leave ethanolic extract against electro- and chemoconvulsant-induced seizures in mice. M...

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Autores principales: Rashidian, Amir, Farhang, Forogh, Vahedi, Habib, Dehpour, Ahmad Reza, Ejtemai Mehr, Shahram, Mehrzadi, Saeed, Rezayat, Seyed Mahdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4977972/
https://www.ncbi.nlm.nih.gov/pubmed/27563433
http://dx.doi.org/10.4103/2008-7802.187251
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author Rashidian, Amir
Farhang, Forogh
Vahedi, Habib
Dehpour, Ahmad Reza
Ejtemai Mehr, Shahram
Mehrzadi, Saeed
Rezayat, Seyed Mahdi
author_facet Rashidian, Amir
Farhang, Forogh
Vahedi, Habib
Dehpour, Ahmad Reza
Ejtemai Mehr, Shahram
Mehrzadi, Saeed
Rezayat, Seyed Mahdi
author_sort Rashidian, Amir
collection PubMed
description BACKGROUND: Lippia citriodora Kunth is one of the Iranian traditional medicines for the treatment of convulsive disorders. The goal of this study is to investigate the anticonvulsant activity of the plant's leave ethanolic extract against electro- and chemoconvulsant-induced seizures in mice. METHODS: The anticonvulsant activity of the extract (200, 400, 800 mg/kg, per os, p.o.) was investigated in pentylenetetrazole (PTZ) and maximal electroshock (MES)-induced seizures in mice. Diazepam (1 mg/kg) and phenytoin (25 mg/kg) intraperitoneally (i.p.) were used as reference drugs. In addition, for investigating the role of GABAergic system, flumazenil (2 mg/kg, i.p.) was also injected before L. citriodora. RESULTS: The extract had not any toxicity and significantly decreased the duration and increased the latency of the seizures induced by PTZ (90 mg/kg). In the MES test, L. citriodora displayed statistically significant reduction in hind limb tonic extension duration in a nondose-dependent manner. Flumazenil reversed the anticonvulsant activity of the plant's extract in the PTZ model. CONCLUSIONS: The results propose that L. citriodora leave ethanolic extract has anticonvulsant activity against convulsive disorders. It seems that this plant's extract generates its antiseizure effect through GABAergic system potentiation. Further studies will be needed in order to investigate the exact mechanisms of it. Moreover, one may conclude that the present results are in accordance with the positive effect of L. citriodora extract to treat convulsion mentioned in old Iranian literature.
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spelling pubmed-49779722016-08-25 Anticonvulsant Effects of Lippia citriodora (Verbenaceae) Leaves Ethanolic Extract in Mice: Role of GABAergic System Rashidian, Amir Farhang, Forogh Vahedi, Habib Dehpour, Ahmad Reza Ejtemai Mehr, Shahram Mehrzadi, Saeed Rezayat, Seyed Mahdi Int J Prev Med Original Article BACKGROUND: Lippia citriodora Kunth is one of the Iranian traditional medicines for the treatment of convulsive disorders. The goal of this study is to investigate the anticonvulsant activity of the plant's leave ethanolic extract against electro- and chemoconvulsant-induced seizures in mice. METHODS: The anticonvulsant activity of the extract (200, 400, 800 mg/kg, per os, p.o.) was investigated in pentylenetetrazole (PTZ) and maximal electroshock (MES)-induced seizures in mice. Diazepam (1 mg/kg) and phenytoin (25 mg/kg) intraperitoneally (i.p.) were used as reference drugs. In addition, for investigating the role of GABAergic system, flumazenil (2 mg/kg, i.p.) was also injected before L. citriodora. RESULTS: The extract had not any toxicity and significantly decreased the duration and increased the latency of the seizures induced by PTZ (90 mg/kg). In the MES test, L. citriodora displayed statistically significant reduction in hind limb tonic extension duration in a nondose-dependent manner. Flumazenil reversed the anticonvulsant activity of the plant's extract in the PTZ model. CONCLUSIONS: The results propose that L. citriodora leave ethanolic extract has anticonvulsant activity against convulsive disorders. It seems that this plant's extract generates its antiseizure effect through GABAergic system potentiation. Further studies will be needed in order to investigate the exact mechanisms of it. Moreover, one may conclude that the present results are in accordance with the positive effect of L. citriodora extract to treat convulsion mentioned in old Iranian literature. Medknow Publications & Media Pvt Ltd 2016-07-29 /pmc/articles/PMC4977972/ /pubmed/27563433 http://dx.doi.org/10.4103/2008-7802.187251 Text en Copyright: © 2016 International Journal of Preventive Medicine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Rashidian, Amir
Farhang, Forogh
Vahedi, Habib
Dehpour, Ahmad Reza
Ejtemai Mehr, Shahram
Mehrzadi, Saeed
Rezayat, Seyed Mahdi
Anticonvulsant Effects of Lippia citriodora (Verbenaceae) Leaves Ethanolic Extract in Mice: Role of GABAergic System
title Anticonvulsant Effects of Lippia citriodora (Verbenaceae) Leaves Ethanolic Extract in Mice: Role of GABAergic System
title_full Anticonvulsant Effects of Lippia citriodora (Verbenaceae) Leaves Ethanolic Extract in Mice: Role of GABAergic System
title_fullStr Anticonvulsant Effects of Lippia citriodora (Verbenaceae) Leaves Ethanolic Extract in Mice: Role of GABAergic System
title_full_unstemmed Anticonvulsant Effects of Lippia citriodora (Verbenaceae) Leaves Ethanolic Extract in Mice: Role of GABAergic System
title_short Anticonvulsant Effects of Lippia citriodora (Verbenaceae) Leaves Ethanolic Extract in Mice: Role of GABAergic System
title_sort anticonvulsant effects of lippia citriodora (verbenaceae) leaves ethanolic extract in mice: role of gabaergic system
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4977972/
https://www.ncbi.nlm.nih.gov/pubmed/27563433
http://dx.doi.org/10.4103/2008-7802.187251
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