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Study of Antiobesity Effect through Inhibition of Pancreatic Lipase Activity of Diospyros kaki Fruit and Citrus unshiu Peel

Pancreatic lipase is the enzyme responsible for digestion and absorption of triglycerides, being its inhibition one of the widest studied methods used to determine the potential activity of natural products to inhibit dietary fat absorption. Decrease of energy intake from dietary fat through inhibit...

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Autores principales: Kim, Gyo-Nam, Shin, Mi-Rae, Shin, Sung Ho, Lee, Ah Reum, Lee, Joo Young, Seo, Bu-Il, Kim, Min Yeong, Kim, Tae Hoon, Noh, Jeong Sook, Rhee, Man Hee, Roh, Seong-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4978832/
https://www.ncbi.nlm.nih.gov/pubmed/27529064
http://dx.doi.org/10.1155/2016/1723042
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author Kim, Gyo-Nam
Shin, Mi-Rae
Shin, Sung Ho
Lee, Ah Reum
Lee, Joo Young
Seo, Bu-Il
Kim, Min Yeong
Kim, Tae Hoon
Noh, Jeong Sook
Rhee, Man Hee
Roh, Seong-Soo
author_facet Kim, Gyo-Nam
Shin, Mi-Rae
Shin, Sung Ho
Lee, Ah Reum
Lee, Joo Young
Seo, Bu-Il
Kim, Min Yeong
Kim, Tae Hoon
Noh, Jeong Sook
Rhee, Man Hee
Roh, Seong-Soo
author_sort Kim, Gyo-Nam
collection PubMed
description Pancreatic lipase is the enzyme responsible for digestion and absorption of triglycerides, being its inhibition one of the widest studied methods used to determine the potential activity of natural products to inhibit dietary fat absorption. Decrease of energy intake from dietary fat through inhibition of this enzyme may be an excellent strategy to prevent and treat obesity. The inhibitory activity on pancreatic lipase enzyme of Diospyros kaki fruit and Citrus unshiu peel mixture extract (PCM) was evaluated in vitro and its antiobesity effects were studied based on the serum lipid parameters analysis from high-fat diet- (HFD-) fed mice in vivo. PCM was orally administered at a dose of 50 and 200 mg/kg body weight for 6 weeks. In addition, the activity of pancreatic lipase was assessed using orlistat (positive control). PCM exhibited inhibitory effect on lipase activity with IC(50) value of 507.01 μg/mL. Moreover, serum triacylglycerol, total cholesterol levels, and visceral fat weight were significantly reduced compared to HFD control mice in PCM 200 mg/kg-treated mice (p < 0.05). These results suggest that PCM administration may be a novel potential antiobesity agent for reduction of fat absorption via inhibition of pancreatic lipase.
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spelling pubmed-49788322016-08-15 Study of Antiobesity Effect through Inhibition of Pancreatic Lipase Activity of Diospyros kaki Fruit and Citrus unshiu Peel Kim, Gyo-Nam Shin, Mi-Rae Shin, Sung Ho Lee, Ah Reum Lee, Joo Young Seo, Bu-Il Kim, Min Yeong Kim, Tae Hoon Noh, Jeong Sook Rhee, Man Hee Roh, Seong-Soo Biomed Res Int Research Article Pancreatic lipase is the enzyme responsible for digestion and absorption of triglycerides, being its inhibition one of the widest studied methods used to determine the potential activity of natural products to inhibit dietary fat absorption. Decrease of energy intake from dietary fat through inhibition of this enzyme may be an excellent strategy to prevent and treat obesity. The inhibitory activity on pancreatic lipase enzyme of Diospyros kaki fruit and Citrus unshiu peel mixture extract (PCM) was evaluated in vitro and its antiobesity effects were studied based on the serum lipid parameters analysis from high-fat diet- (HFD-) fed mice in vivo. PCM was orally administered at a dose of 50 and 200 mg/kg body weight for 6 weeks. In addition, the activity of pancreatic lipase was assessed using orlistat (positive control). PCM exhibited inhibitory effect on lipase activity with IC(50) value of 507.01 μg/mL. Moreover, serum triacylglycerol, total cholesterol levels, and visceral fat weight were significantly reduced compared to HFD control mice in PCM 200 mg/kg-treated mice (p < 0.05). These results suggest that PCM administration may be a novel potential antiobesity agent for reduction of fat absorption via inhibition of pancreatic lipase. Hindawi Publishing Corporation 2016 2016-07-27 /pmc/articles/PMC4978832/ /pubmed/27529064 http://dx.doi.org/10.1155/2016/1723042 Text en Copyright © 2016 Gyo-Nam Kim et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kim, Gyo-Nam
Shin, Mi-Rae
Shin, Sung Ho
Lee, Ah Reum
Lee, Joo Young
Seo, Bu-Il
Kim, Min Yeong
Kim, Tae Hoon
Noh, Jeong Sook
Rhee, Man Hee
Roh, Seong-Soo
Study of Antiobesity Effect through Inhibition of Pancreatic Lipase Activity of Diospyros kaki Fruit and Citrus unshiu Peel
title Study of Antiobesity Effect through Inhibition of Pancreatic Lipase Activity of Diospyros kaki Fruit and Citrus unshiu Peel
title_full Study of Antiobesity Effect through Inhibition of Pancreatic Lipase Activity of Diospyros kaki Fruit and Citrus unshiu Peel
title_fullStr Study of Antiobesity Effect through Inhibition of Pancreatic Lipase Activity of Diospyros kaki Fruit and Citrus unshiu Peel
title_full_unstemmed Study of Antiobesity Effect through Inhibition of Pancreatic Lipase Activity of Diospyros kaki Fruit and Citrus unshiu Peel
title_short Study of Antiobesity Effect through Inhibition of Pancreatic Lipase Activity of Diospyros kaki Fruit and Citrus unshiu Peel
title_sort study of antiobesity effect through inhibition of pancreatic lipase activity of diospyros kaki fruit and citrus unshiu peel
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4978832/
https://www.ncbi.nlm.nih.gov/pubmed/27529064
http://dx.doi.org/10.1155/2016/1723042
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