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Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response

Both bone marrow and adipose-derived mesenchymal stem cells (ASCs) have immunomodulatory effects. The goal of this study was to determine whether ASCs-educated macrophages could directly ameliorate LPS-induced systemic response in a mouse model. Mouse peritoneal macrophages were cocultured with ASCs...

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Autores principales: Hu, Yaoqin, Qin, Chaojin, Zheng, Guoping, Lai, Dengming, Tao, Huikang, Zhang, Yan, Qiu, Guanguan, Ge, Menghua, Huang, Lanfang, Chen, Lina, Cheng, Baoli, Shu, Qiang, Xu, Jianguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4978851/
https://www.ncbi.nlm.nih.gov/pubmed/27546994
http://dx.doi.org/10.1155/2016/3735452
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author Hu, Yaoqin
Qin, Chaojin
Zheng, Guoping
Lai, Dengming
Tao, Huikang
Zhang, Yan
Qiu, Guanguan
Ge, Menghua
Huang, Lanfang
Chen, Lina
Cheng, Baoli
Shu, Qiang
Xu, Jianguo
author_facet Hu, Yaoqin
Qin, Chaojin
Zheng, Guoping
Lai, Dengming
Tao, Huikang
Zhang, Yan
Qiu, Guanguan
Ge, Menghua
Huang, Lanfang
Chen, Lina
Cheng, Baoli
Shu, Qiang
Xu, Jianguo
author_sort Hu, Yaoqin
collection PubMed
description Both bone marrow and adipose-derived mesenchymal stem cells (ASCs) have immunomodulatory effects. The goal of this study was to determine whether ASCs-educated macrophages could directly ameliorate LPS-induced systemic response in a mouse model. Mouse peritoneal macrophages were cocultured with ASCs in a Transwell system for 2 days to educate macrophages. Mice were divided into 5 groups: control, LPS, LPS + ASCs, LPS + untreated macrophages, and LPS + educated macrophages. Educated macrophages decreased lung inflammation, weight loss, pulmonary edema, and inflammatory cytokine response. In vitro, ASCs increased expression of M2 macrophages independent of direct cell-to-cell contact when macrophages were treated with LPS or serum from patients with acute respiratory distress syndrome (ARDS). When macrophages were cultured with serum from ARDS patients who were treated with ASCs or placebo in our previous clinical trial, there was no difference in M2 macrophage levels before and after ASCs treatment indicating a suboptimal response to the treatment protocol. ASCs also reduced the levels of LPS-induced proinflammatory cytokines in vitro which were mimicked by IL-10 and blocked by antibodies for IL-10 and IL-10 receptor supporting the notion that educated macrophages exert their anti-inflammatory effects via IL-10-dependent mechanisms.
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spelling pubmed-49788512016-08-21 Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response Hu, Yaoqin Qin, Chaojin Zheng, Guoping Lai, Dengming Tao, Huikang Zhang, Yan Qiu, Guanguan Ge, Menghua Huang, Lanfang Chen, Lina Cheng, Baoli Shu, Qiang Xu, Jianguo Mediators Inflamm Research Article Both bone marrow and adipose-derived mesenchymal stem cells (ASCs) have immunomodulatory effects. The goal of this study was to determine whether ASCs-educated macrophages could directly ameliorate LPS-induced systemic response in a mouse model. Mouse peritoneal macrophages were cocultured with ASCs in a Transwell system for 2 days to educate macrophages. Mice were divided into 5 groups: control, LPS, LPS + ASCs, LPS + untreated macrophages, and LPS + educated macrophages. Educated macrophages decreased lung inflammation, weight loss, pulmonary edema, and inflammatory cytokine response. In vitro, ASCs increased expression of M2 macrophages independent of direct cell-to-cell contact when macrophages were treated with LPS or serum from patients with acute respiratory distress syndrome (ARDS). When macrophages were cultured with serum from ARDS patients who were treated with ASCs or placebo in our previous clinical trial, there was no difference in M2 macrophage levels before and after ASCs treatment indicating a suboptimal response to the treatment protocol. ASCs also reduced the levels of LPS-induced proinflammatory cytokines in vitro which were mimicked by IL-10 and blocked by antibodies for IL-10 and IL-10 receptor supporting the notion that educated macrophages exert their anti-inflammatory effects via IL-10-dependent mechanisms. Hindawi Publishing Corporation 2016 2016-07-27 /pmc/articles/PMC4978851/ /pubmed/27546994 http://dx.doi.org/10.1155/2016/3735452 Text en Copyright © 2016 Yaoqin Hu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hu, Yaoqin
Qin, Chaojin
Zheng, Guoping
Lai, Dengming
Tao, Huikang
Zhang, Yan
Qiu, Guanguan
Ge, Menghua
Huang, Lanfang
Chen, Lina
Cheng, Baoli
Shu, Qiang
Xu, Jianguo
Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response
title Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response
title_full Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response
title_fullStr Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response
title_full_unstemmed Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response
title_short Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response
title_sort mesenchymal stem cell-educated macrophages ameliorate lps-induced systemic response
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4978851/
https://www.ncbi.nlm.nih.gov/pubmed/27546994
http://dx.doi.org/10.1155/2016/3735452
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