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External RNA Controls Consortium Beta Version Update

Spike-in RNAs are valuable controls for a variety of gene expression measurements. The External RNA Controls Consortium developed test sets that were used in a number of published reports. Here we provide an authoritative table that summarizes, updates, and corrects errors in the test version that u...

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Detalles Bibliográficos
Autores principales: Lee, Hangnoh, Pine, P. Scott, McDaniel, Jennifer, Salit, Marc, Oliver, Brian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4978944/
https://www.ncbi.nlm.nih.gov/pubmed/27512518
http://dx.doi.org/10.7150/jgen.16082
Descripción
Sumario:Spike-in RNAs are valuable controls for a variety of gene expression measurements. The External RNA Controls Consortium developed test sets that were used in a number of published reports. Here we provide an authoritative table that summarizes, updates, and corrects errors in the test version that ultimately resulted in the certified Standard Reference Material 2374. We have noted existence of anti-sense RNA controls in the material, corrected sub-pool memberships, and commented on control RNAs that displayed inconsistent behavior.