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Controllable in-situ cell electroporation with cell positioning and impedance monitoring using micro electrode array

This paper reports a novel microarray chip for in-situ, real-time and selective electroporation on individual cells integrated with cell positioning and impedance monitoring. An array of quadrupole-electrode units (termed positioning electrodes) and pairs of planar center electrodes located at the c...

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Detalles Bibliográficos
Autores principales: Guo, Xiaoliang, Zhu, Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4979028/
https://www.ncbi.nlm.nih.gov/pubmed/27507603
http://dx.doi.org/10.1038/srep31392
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author Guo, Xiaoliang
Zhu, Rong
author_facet Guo, Xiaoliang
Zhu, Rong
author_sort Guo, Xiaoliang
collection PubMed
description This paper reports a novel microarray chip for in-situ, real-time and selective electroporation on individual cells integrated with cell positioning and impedance monitoring. An array of quadrupole-electrode units (termed positioning electrodes) and pairs of planar center electrodes located at the centers of each quadrupole-electrode unit were fabricated on the chip. The positioning electrodes are used to trap and position living cells onto the center electrodes based on negative dielectrophoresis (nDEP). The center electrodes are used for in-situ cell electroporation, and also used to measure cell impedance for monitoring cellular dynamics in real time. Controllably selective electroporation and electrical measurement on the cells in array are realized. We present an evidence of selective electroporation through use of fluorescent dyes. Subsequently we use in-situ and real-time impedance measurement to monitor the process, which demonstrates the dynamic behavior of the cell electroporation. Finally, we show the use of this device to perform successful transfection onto individual HeLa cells with vector DNA encoding a green fluorescent.
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spelling pubmed-49790282016-08-19 Controllable in-situ cell electroporation with cell positioning and impedance monitoring using micro electrode array Guo, Xiaoliang Zhu, Rong Sci Rep Article This paper reports a novel microarray chip for in-situ, real-time and selective electroporation on individual cells integrated with cell positioning and impedance monitoring. An array of quadrupole-electrode units (termed positioning electrodes) and pairs of planar center electrodes located at the centers of each quadrupole-electrode unit were fabricated on the chip. The positioning electrodes are used to trap and position living cells onto the center electrodes based on negative dielectrophoresis (nDEP). The center electrodes are used for in-situ cell electroporation, and also used to measure cell impedance for monitoring cellular dynamics in real time. Controllably selective electroporation and electrical measurement on the cells in array are realized. We present an evidence of selective electroporation through use of fluorescent dyes. Subsequently we use in-situ and real-time impedance measurement to monitor the process, which demonstrates the dynamic behavior of the cell electroporation. Finally, we show the use of this device to perform successful transfection onto individual HeLa cells with vector DNA encoding a green fluorescent. Nature Publishing Group 2016-08-10 /pmc/articles/PMC4979028/ /pubmed/27507603 http://dx.doi.org/10.1038/srep31392 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Guo, Xiaoliang
Zhu, Rong
Controllable in-situ cell electroporation with cell positioning and impedance monitoring using micro electrode array
title Controllable in-situ cell electroporation with cell positioning and impedance monitoring using micro electrode array
title_full Controllable in-situ cell electroporation with cell positioning and impedance monitoring using micro electrode array
title_fullStr Controllable in-situ cell electroporation with cell positioning and impedance monitoring using micro electrode array
title_full_unstemmed Controllable in-situ cell electroporation with cell positioning and impedance monitoring using micro electrode array
title_short Controllable in-situ cell electroporation with cell positioning and impedance monitoring using micro electrode array
title_sort controllable in-situ cell electroporation with cell positioning and impedance monitoring using micro electrode array
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4979028/
https://www.ncbi.nlm.nih.gov/pubmed/27507603
http://dx.doi.org/10.1038/srep31392
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