Cargando…
DNA Microarray Analysis on the Genes Differentially Expressed in the Liver of the Pufferfish, Takifugu rubripes, Following an Intramuscular Administration of Tetrodotoxin
Pufferfish accumulate tetrodotoxin (TTX) mainly in the liver and ovary. This study aims at investigating the effect of TTX accumulation in the liver of cultured specimens of torafugu Takifugu rubripes on the hepatic gene expression by microarray analysis on Day 5 after the intramuscular administrati...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4979056/ https://www.ncbi.nlm.nih.gov/pubmed/27600346 http://dx.doi.org/10.3390/microarrays3040226 |
_version_ | 1782447264943308800 |
---|---|
author | Matsumoto, Takuya Feroudj, Holger Kikuchi, Ryosuke Kawana, Yuriko Kondo, Hidehiro Hirono, Ikuo Mochizuki, Toshiaki Nagashima, Yuji Kaneko, Gen Ushio, Hideki Kodama, Masaaki Watabe, Shugo |
author_facet | Matsumoto, Takuya Feroudj, Holger Kikuchi, Ryosuke Kawana, Yuriko Kondo, Hidehiro Hirono, Ikuo Mochizuki, Toshiaki Nagashima, Yuji Kaneko, Gen Ushio, Hideki Kodama, Masaaki Watabe, Shugo |
author_sort | Matsumoto, Takuya |
collection | PubMed |
description | Pufferfish accumulate tetrodotoxin (TTX) mainly in the liver and ovary. This study aims at investigating the effect of TTX accumulation in the liver of cultured specimens of torafugu Takifugu rubripes on the hepatic gene expression by microarray analysis on Day 5 after the intramuscular administration of 0.25 mg TTX/kg body weight into the caudal muscle. TTX was detected in the liver, skin and ovary in the TTX-administered individuals. The total amount of TTX accumulated in the body was 67 ± 8% of the administered dose on Day 5. Compared with the buffer-administered control group, a total of 59 genes were significantly upregulated more than two-fold in the TTX-administered group, including those encoding chymotrypsin-like elastase family member 2A, transmembrane protein 168 and Rho GTP-activating protein 29. In contrast, a total of 427 genes were downregulated by TTX administration, including those encoding elongation factor G2, R-spondin-3, nuclear receptor activator 2 and fatty acyl-CoA hydrolase precursor. In conclusion, our results demonstrate that the intramuscular administration of TTX changes the expression of hepatic genes involved in various signaling pathways. |
format | Online Article Text |
id | pubmed-4979056 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-49790562016-09-06 DNA Microarray Analysis on the Genes Differentially Expressed in the Liver of the Pufferfish, Takifugu rubripes, Following an Intramuscular Administration of Tetrodotoxin Matsumoto, Takuya Feroudj, Holger Kikuchi, Ryosuke Kawana, Yuriko Kondo, Hidehiro Hirono, Ikuo Mochizuki, Toshiaki Nagashima, Yuji Kaneko, Gen Ushio, Hideki Kodama, Masaaki Watabe, Shugo Microarrays (Basel) Article Pufferfish accumulate tetrodotoxin (TTX) mainly in the liver and ovary. This study aims at investigating the effect of TTX accumulation in the liver of cultured specimens of torafugu Takifugu rubripes on the hepatic gene expression by microarray analysis on Day 5 after the intramuscular administration of 0.25 mg TTX/kg body weight into the caudal muscle. TTX was detected in the liver, skin and ovary in the TTX-administered individuals. The total amount of TTX accumulated in the body was 67 ± 8% of the administered dose on Day 5. Compared with the buffer-administered control group, a total of 59 genes were significantly upregulated more than two-fold in the TTX-administered group, including those encoding chymotrypsin-like elastase family member 2A, transmembrane protein 168 and Rho GTP-activating protein 29. In contrast, a total of 427 genes were downregulated by TTX administration, including those encoding elongation factor G2, R-spondin-3, nuclear receptor activator 2 and fatty acyl-CoA hydrolase precursor. In conclusion, our results demonstrate that the intramuscular administration of TTX changes the expression of hepatic genes involved in various signaling pathways. MDPI 2014-10-27 /pmc/articles/PMC4979056/ /pubmed/27600346 http://dx.doi.org/10.3390/microarrays3040226 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Matsumoto, Takuya Feroudj, Holger Kikuchi, Ryosuke Kawana, Yuriko Kondo, Hidehiro Hirono, Ikuo Mochizuki, Toshiaki Nagashima, Yuji Kaneko, Gen Ushio, Hideki Kodama, Masaaki Watabe, Shugo DNA Microarray Analysis on the Genes Differentially Expressed in the Liver of the Pufferfish, Takifugu rubripes, Following an Intramuscular Administration of Tetrodotoxin |
title | DNA Microarray Analysis on the Genes Differentially Expressed in the Liver of the Pufferfish, Takifugu rubripes, Following an Intramuscular Administration of Tetrodotoxin |
title_full | DNA Microarray Analysis on the Genes Differentially Expressed in the Liver of the Pufferfish, Takifugu rubripes, Following an Intramuscular Administration of Tetrodotoxin |
title_fullStr | DNA Microarray Analysis on the Genes Differentially Expressed in the Liver of the Pufferfish, Takifugu rubripes, Following an Intramuscular Administration of Tetrodotoxin |
title_full_unstemmed | DNA Microarray Analysis on the Genes Differentially Expressed in the Liver of the Pufferfish, Takifugu rubripes, Following an Intramuscular Administration of Tetrodotoxin |
title_short | DNA Microarray Analysis on the Genes Differentially Expressed in the Liver of the Pufferfish, Takifugu rubripes, Following an Intramuscular Administration of Tetrodotoxin |
title_sort | dna microarray analysis on the genes differentially expressed in the liver of the pufferfish, takifugu rubripes, following an intramuscular administration of tetrodotoxin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4979056/ https://www.ncbi.nlm.nih.gov/pubmed/27600346 http://dx.doi.org/10.3390/microarrays3040226 |
work_keys_str_mv | AT matsumototakuya dnamicroarrayanalysisonthegenesdifferentiallyexpressedintheliverofthepufferfishtakifugurubripesfollowinganintramuscularadministrationoftetrodotoxin AT feroudjholger dnamicroarrayanalysisonthegenesdifferentiallyexpressedintheliverofthepufferfishtakifugurubripesfollowinganintramuscularadministrationoftetrodotoxin AT kikuchiryosuke dnamicroarrayanalysisonthegenesdifferentiallyexpressedintheliverofthepufferfishtakifugurubripesfollowinganintramuscularadministrationoftetrodotoxin AT kawanayuriko dnamicroarrayanalysisonthegenesdifferentiallyexpressedintheliverofthepufferfishtakifugurubripesfollowinganintramuscularadministrationoftetrodotoxin AT kondohidehiro dnamicroarrayanalysisonthegenesdifferentiallyexpressedintheliverofthepufferfishtakifugurubripesfollowinganintramuscularadministrationoftetrodotoxin AT hironoikuo dnamicroarrayanalysisonthegenesdifferentiallyexpressedintheliverofthepufferfishtakifugurubripesfollowinganintramuscularadministrationoftetrodotoxin AT mochizukitoshiaki dnamicroarrayanalysisonthegenesdifferentiallyexpressedintheliverofthepufferfishtakifugurubripesfollowinganintramuscularadministrationoftetrodotoxin AT nagashimayuji dnamicroarrayanalysisonthegenesdifferentiallyexpressedintheliverofthepufferfishtakifugurubripesfollowinganintramuscularadministrationoftetrodotoxin AT kanekogen dnamicroarrayanalysisonthegenesdifferentiallyexpressedintheliverofthepufferfishtakifugurubripesfollowinganintramuscularadministrationoftetrodotoxin AT ushiohideki dnamicroarrayanalysisonthegenesdifferentiallyexpressedintheliverofthepufferfishtakifugurubripesfollowinganintramuscularadministrationoftetrodotoxin AT kodamamasaaki dnamicroarrayanalysisonthegenesdifferentiallyexpressedintheliverofthepufferfishtakifugurubripesfollowinganintramuscularadministrationoftetrodotoxin AT watabeshugo dnamicroarrayanalysisonthegenesdifferentiallyexpressedintheliverofthepufferfishtakifugurubripesfollowinganintramuscularadministrationoftetrodotoxin |