Cargando…

Easy-to-Use Preservation and Application of Platelet-Rich Plasma in Combination Wound Therapy With a Gelatin Sheet and Freeze-Dried Platelet-Rich Plasma: A Case Report

Objective: Platelet-rich plasma is blood plasma enriched with platelets and contains various growth factors. Two major issues remain to be resolved in the use of platelet-rich plasma: the short biological activity after application, and the need to prepare platelet-rich plasma at each application in...

Descripción completa

Detalles Bibliográficos
Autores principales: Morimoto, Naoki, Kakudo, Natsuko, Ogura, Tsunekata, Hara, Tomoya, Matsui, Makoto, Yamamoto, Masaya, Tabata, Yasuhiko, Kusumoto, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Open Science Company, LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4979162/
https://www.ncbi.nlm.nih.gov/pubmed/27555889
_version_ 1782447281388126208
author Morimoto, Naoki
Kakudo, Natsuko
Ogura, Tsunekata
Hara, Tomoya
Matsui, Makoto
Yamamoto, Masaya
Tabata, Yasuhiko
Kusumoto, Kenji
author_facet Morimoto, Naoki
Kakudo, Natsuko
Ogura, Tsunekata
Hara, Tomoya
Matsui, Makoto
Yamamoto, Masaya
Tabata, Yasuhiko
Kusumoto, Kenji
author_sort Morimoto, Naoki
collection PubMed
description Objective: Platelet-rich plasma is blood plasma enriched with platelets and contains various growth factors. Two major issues remain to be resolved in the use of platelet-rich plasma: the short biological activity after application, and the need to prepare platelet-rich plasma at each application instance. To overcome these problems, we developed a drug delivery system using gelatin hydrogel and preserved the excess platelet-rich plasma as freeze-dried platelet-rich plasma. We then applied combination treatment with a gelatin sheet and platelet-rich plasma at the first instance and freeze-dried platelet-rich plasma at the second instance in the treatment of a nonhealing wound. Methods: A 68-year-old woman had suffered open fracture of her right tibia 2 years prior, and a split-thickness skin graft had been applied to repair the skin defect on the right tibia. She had multiple relapse of ulcers, and the present ulcer had not healed for 2 months. After debridement, 2 mL of activated platelet-rich plasma was applied to the ulcer, and the gelatin sheet was laid to impregnate with the platelet-rich plasma, after which the sheet was covered with a polyurethane film. Thirty-three days after the first platelet-rich plasma application, the freeze-dried platelet-rich plasma was reconstituted and 2 mL of the reconstituted platelet-rich plasma was applied with a gelatin sheet. Results: At 14 days after the freeze-dried platelet-rich plasma application, the wound was mostly epithelized, with the rest of the wound covered with granulation tissue. Conclusions: These findings suggest that combination wound therapy with a gelatin sheet and freeze-dried platelet-rich plasma is a promising method for resolving issues with conventional platelet-rich plasma treatment.
format Online
Article
Text
id pubmed-4979162
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Open Science Company, LLC
record_format MEDLINE/PubMed
spelling pubmed-49791622016-08-23 Easy-to-Use Preservation and Application of Platelet-Rich Plasma in Combination Wound Therapy With a Gelatin Sheet and Freeze-Dried Platelet-Rich Plasma: A Case Report Morimoto, Naoki Kakudo, Natsuko Ogura, Tsunekata Hara, Tomoya Matsui, Makoto Yamamoto, Masaya Tabata, Yasuhiko Kusumoto, Kenji Eplasty Case Report Objective: Platelet-rich plasma is blood plasma enriched with platelets and contains various growth factors. Two major issues remain to be resolved in the use of platelet-rich plasma: the short biological activity after application, and the need to prepare platelet-rich plasma at each application instance. To overcome these problems, we developed a drug delivery system using gelatin hydrogel and preserved the excess platelet-rich plasma as freeze-dried platelet-rich plasma. We then applied combination treatment with a gelatin sheet and platelet-rich plasma at the first instance and freeze-dried platelet-rich plasma at the second instance in the treatment of a nonhealing wound. Methods: A 68-year-old woman had suffered open fracture of her right tibia 2 years prior, and a split-thickness skin graft had been applied to repair the skin defect on the right tibia. She had multiple relapse of ulcers, and the present ulcer had not healed for 2 months. After debridement, 2 mL of activated platelet-rich plasma was applied to the ulcer, and the gelatin sheet was laid to impregnate with the platelet-rich plasma, after which the sheet was covered with a polyurethane film. Thirty-three days after the first platelet-rich plasma application, the freeze-dried platelet-rich plasma was reconstituted and 2 mL of the reconstituted platelet-rich plasma was applied with a gelatin sheet. Results: At 14 days after the freeze-dried platelet-rich plasma application, the wound was mostly epithelized, with the rest of the wound covered with granulation tissue. Conclusions: These findings suggest that combination wound therapy with a gelatin sheet and freeze-dried platelet-rich plasma is a promising method for resolving issues with conventional platelet-rich plasma treatment. Open Science Company, LLC 2016-08-05 /pmc/articles/PMC4979162/ /pubmed/27555889 Text en Copyright © 2016 The Author(s) http://creativecommons.org/licenses/by/2.0/ This is an open-access article whereby the authors retain copyright of the work. The article is distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Case Report
Morimoto, Naoki
Kakudo, Natsuko
Ogura, Tsunekata
Hara, Tomoya
Matsui, Makoto
Yamamoto, Masaya
Tabata, Yasuhiko
Kusumoto, Kenji
Easy-to-Use Preservation and Application of Platelet-Rich Plasma in Combination Wound Therapy With a Gelatin Sheet and Freeze-Dried Platelet-Rich Plasma: A Case Report
title Easy-to-Use Preservation and Application of Platelet-Rich Plasma in Combination Wound Therapy With a Gelatin Sheet and Freeze-Dried Platelet-Rich Plasma: A Case Report
title_full Easy-to-Use Preservation and Application of Platelet-Rich Plasma in Combination Wound Therapy With a Gelatin Sheet and Freeze-Dried Platelet-Rich Plasma: A Case Report
title_fullStr Easy-to-Use Preservation and Application of Platelet-Rich Plasma in Combination Wound Therapy With a Gelatin Sheet and Freeze-Dried Platelet-Rich Plasma: A Case Report
title_full_unstemmed Easy-to-Use Preservation and Application of Platelet-Rich Plasma in Combination Wound Therapy With a Gelatin Sheet and Freeze-Dried Platelet-Rich Plasma: A Case Report
title_short Easy-to-Use Preservation and Application of Platelet-Rich Plasma in Combination Wound Therapy With a Gelatin Sheet and Freeze-Dried Platelet-Rich Plasma: A Case Report
title_sort easy-to-use preservation and application of platelet-rich plasma in combination wound therapy with a gelatin sheet and freeze-dried platelet-rich plasma: a case report
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4979162/
https://www.ncbi.nlm.nih.gov/pubmed/27555889
work_keys_str_mv AT morimotonaoki easytousepreservationandapplicationofplateletrichplasmaincombinationwoundtherapywithagelatinsheetandfreezedriedplateletrichplasmaacasereport
AT kakudonatsuko easytousepreservationandapplicationofplateletrichplasmaincombinationwoundtherapywithagelatinsheetandfreezedriedplateletrichplasmaacasereport
AT oguratsunekata easytousepreservationandapplicationofplateletrichplasmaincombinationwoundtherapywithagelatinsheetandfreezedriedplateletrichplasmaacasereport
AT haratomoya easytousepreservationandapplicationofplateletrichplasmaincombinationwoundtherapywithagelatinsheetandfreezedriedplateletrichplasmaacasereport
AT matsuimakoto easytousepreservationandapplicationofplateletrichplasmaincombinationwoundtherapywithagelatinsheetandfreezedriedplateletrichplasmaacasereport
AT yamamotomasaya easytousepreservationandapplicationofplateletrichplasmaincombinationwoundtherapywithagelatinsheetandfreezedriedplateletrichplasmaacasereport
AT tabatayasuhiko easytousepreservationandapplicationofplateletrichplasmaincombinationwoundtherapywithagelatinsheetandfreezedriedplateletrichplasmaacasereport
AT kusumotokenji easytousepreservationandapplicationofplateletrichplasmaincombinationwoundtherapywithagelatinsheetandfreezedriedplateletrichplasmaacasereport