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Chromosome doubling to overcome the chrysanthemum cross barrier based on insight from transcriptomic and proteomic analyses

BACKGROUND: Cross breeding is the most commonly used method in chrysanthemum (Chrysanthemum morifolium) breeding; however, cross barriers always exist in these combinations. Many studies have shown that paternal chromosome doubling can often overcome hybridization barriers during cross breeding, alt...

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Autores principales: Zhang, Fengjiao, Hua, Lichun, Fei, Jiangsong, Wang, Fan, Liao, Yuan, Fang, Weimin, Chen, Fadi, Teng, Nianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4979184/
https://www.ncbi.nlm.nih.gov/pubmed/27506621
http://dx.doi.org/10.1186/s12864-016-2939-0
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author Zhang, Fengjiao
Hua, Lichun
Fei, Jiangsong
Wang, Fan
Liao, Yuan
Fang, Weimin
Chen, Fadi
Teng, Nianjun
author_facet Zhang, Fengjiao
Hua, Lichun
Fei, Jiangsong
Wang, Fan
Liao, Yuan
Fang, Weimin
Chen, Fadi
Teng, Nianjun
author_sort Zhang, Fengjiao
collection PubMed
description BACKGROUND: Cross breeding is the most commonly used method in chrysanthemum (Chrysanthemum morifolium) breeding; however, cross barriers always exist in these combinations. Many studies have shown that paternal chromosome doubling can often overcome hybridization barriers during cross breeding, although the underlying mechanism has seldom been investigated. RESULTS: In this study, we performed two crosses: C. morifolium (pollen receptor) × diploid C. nankingense (pollen donor) and C. morifolium × tetraploid C. nankingense. Seeds were obtained only from the latter cross. RNA-Seq and isobaric tags for relative and absolute quantitation (iTRAQ) were used to investigate differentially expressed genes and proteins during key embryo development stages in the latter cross. A previously performed cross, C. morifolium × diploid C. nankingense, was compared to our results and revealed that transcription factors (i.e., the agamous-like MADS-box protein AGL80 and the leucine-rich repeat receptor protein kinase EXS), hormone-responsive genes (auxin-binding protein 1), genes and proteins related to metabolism (ATP-citrate synthase, citrate synthase and malate dehydrogenase) and other genes reported to contribute to embryo development (i.e., LEA, elongation factor and tubulin) had higher expression levels in the C. morifolium × tetraploid C. nankingense cross. In contrast, genes related to senescence and cell death were down-regulated in the C. morifolium × tetraploid C. nankingense cross. CONCLUSIONS: The data resources helped elucidate the gene and protein expression profiles and identify functional genes during different development stages. When the chromosomes from the male parent are doubled, the genes contributing to normal embryo developmentare more abundant. However, genes with negative functions were suppressed, suggesting that chromosome doubling may epigenetically inhibit the expression of these genes and allow the embryo to develop normally. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-2939-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-49791842016-08-11 Chromosome doubling to overcome the chrysanthemum cross barrier based on insight from transcriptomic and proteomic analyses Zhang, Fengjiao Hua, Lichun Fei, Jiangsong Wang, Fan Liao, Yuan Fang, Weimin Chen, Fadi Teng, Nianjun BMC Genomics Research Article BACKGROUND: Cross breeding is the most commonly used method in chrysanthemum (Chrysanthemum morifolium) breeding; however, cross barriers always exist in these combinations. Many studies have shown that paternal chromosome doubling can often overcome hybridization barriers during cross breeding, although the underlying mechanism has seldom been investigated. RESULTS: In this study, we performed two crosses: C. morifolium (pollen receptor) × diploid C. nankingense (pollen donor) and C. morifolium × tetraploid C. nankingense. Seeds were obtained only from the latter cross. RNA-Seq and isobaric tags for relative and absolute quantitation (iTRAQ) were used to investigate differentially expressed genes and proteins during key embryo development stages in the latter cross. A previously performed cross, C. morifolium × diploid C. nankingense, was compared to our results and revealed that transcription factors (i.e., the agamous-like MADS-box protein AGL80 and the leucine-rich repeat receptor protein kinase EXS), hormone-responsive genes (auxin-binding protein 1), genes and proteins related to metabolism (ATP-citrate synthase, citrate synthase and malate dehydrogenase) and other genes reported to contribute to embryo development (i.e., LEA, elongation factor and tubulin) had higher expression levels in the C. morifolium × tetraploid C. nankingense cross. In contrast, genes related to senescence and cell death were down-regulated in the C. morifolium × tetraploid C. nankingense cross. CONCLUSIONS: The data resources helped elucidate the gene and protein expression profiles and identify functional genes during different development stages. When the chromosomes from the male parent are doubled, the genes contributing to normal embryo developmentare more abundant. However, genes with negative functions were suppressed, suggesting that chromosome doubling may epigenetically inhibit the expression of these genes and allow the embryo to develop normally. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-2939-0) contains supplementary material, which is available to authorized users. BioMed Central 2016-08-09 /pmc/articles/PMC4979184/ /pubmed/27506621 http://dx.doi.org/10.1186/s12864-016-2939-0 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhang, Fengjiao
Hua, Lichun
Fei, Jiangsong
Wang, Fan
Liao, Yuan
Fang, Weimin
Chen, Fadi
Teng, Nianjun
Chromosome doubling to overcome the chrysanthemum cross barrier based on insight from transcriptomic and proteomic analyses
title Chromosome doubling to overcome the chrysanthemum cross barrier based on insight from transcriptomic and proteomic analyses
title_full Chromosome doubling to overcome the chrysanthemum cross barrier based on insight from transcriptomic and proteomic analyses
title_fullStr Chromosome doubling to overcome the chrysanthemum cross barrier based on insight from transcriptomic and proteomic analyses
title_full_unstemmed Chromosome doubling to overcome the chrysanthemum cross barrier based on insight from transcriptomic and proteomic analyses
title_short Chromosome doubling to overcome the chrysanthemum cross barrier based on insight from transcriptomic and proteomic analyses
title_sort chromosome doubling to overcome the chrysanthemum cross barrier based on insight from transcriptomic and proteomic analyses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4979184/
https://www.ncbi.nlm.nih.gov/pubmed/27506621
http://dx.doi.org/10.1186/s12864-016-2939-0
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