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Effect of melatonin on maturation capacity and fertilization of Nili-Ravi buffalo (Bubalus bubalis) oocytes
This study evaluated the effect of melatonin supplementation of in vitro maturation media on in vitro maturation (IVM) and in vitro fertilization (IVF) rate of buffalo oocytes. Cumulus oocytes complexes (COCs) were aspirated from follicles of 2-8 mm diameter. In experiment I, COCs were matured in IV...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980478/ https://www.ncbi.nlm.nih.gov/pubmed/27540514 http://dx.doi.org/10.4314/ovj.v6i2.9 |
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author | Nagina, G. Asima, A. Nemat, U. Shamim, A. |
author_facet | Nagina, G. Asima, A. Nemat, U. Shamim, A. |
author_sort | Nagina, G. |
collection | PubMed |
description | This study evaluated the effect of melatonin supplementation of in vitro maturation media on in vitro maturation (IVM) and in vitro fertilization (IVF) rate of buffalo oocytes. Cumulus oocytes complexes (COCs) were aspirated from follicles of 2-8 mm diameter. In experiment I, COCs were matured in IVM medium supplemented with 0 (control), 250, 500, and 1000 μM melatonin for 22-24 hours in CO(2) incubator at 38.5°C with 5% CO(2) and at 95% relative humidity. The maturation rate did not differ in media supplemented with melatonin at 250 μM, 500 μM, 1000 μM and control (0 μM). In experiment II, the matured oocytes were fertilized in 50 μl droplets of Tyrode’s Albumin Lactate Pyruvate (TALP) medium having 10 ug/ml heparin for sperm (2 million/ml) capacitation. The fertilization droplets were then kept for incubation at 5% CO(2), 39°C and at 95% relative humidity for 18 hours. The fertilization rate was assessed by sperm penetration and pronuclear formation. Fertilization rate was improved when maturation medium was supplemented with 250 μM melatonin compared to control. In conclusion, melatonin supplementation to serum free maturation media at 250 μM improved the fertilization rate of buffalo oocytes. |
format | Online Article Text |
id | pubmed-4980478 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology |
record_format | MEDLINE/PubMed |
spelling | pubmed-49804782016-08-18 Effect of melatonin on maturation capacity and fertilization of Nili-Ravi buffalo (Bubalus bubalis) oocytes Nagina, G. Asima, A. Nemat, U. Shamim, A. Open Vet J Original Article This study evaluated the effect of melatonin supplementation of in vitro maturation media on in vitro maturation (IVM) and in vitro fertilization (IVF) rate of buffalo oocytes. Cumulus oocytes complexes (COCs) were aspirated from follicles of 2-8 mm diameter. In experiment I, COCs were matured in IVM medium supplemented with 0 (control), 250, 500, and 1000 μM melatonin for 22-24 hours in CO(2) incubator at 38.5°C with 5% CO(2) and at 95% relative humidity. The maturation rate did not differ in media supplemented with melatonin at 250 μM, 500 μM, 1000 μM and control (0 μM). In experiment II, the matured oocytes were fertilized in 50 μl droplets of Tyrode’s Albumin Lactate Pyruvate (TALP) medium having 10 ug/ml heparin for sperm (2 million/ml) capacitation. The fertilization droplets were then kept for incubation at 5% CO(2), 39°C and at 95% relative humidity for 18 hours. The fertilization rate was assessed by sperm penetration and pronuclear formation. Fertilization rate was improved when maturation medium was supplemented with 250 μM melatonin compared to control. In conclusion, melatonin supplementation to serum free maturation media at 250 μM improved the fertilization rate of buffalo oocytes. Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology 2016 2016-08-04 /pmc/articles/PMC4980478/ /pubmed/27540514 http://dx.doi.org/10.4314/ovj.v6i2.9 Text en Copyright: © Open Veterinary Journal http://creativecommons.org/licenses/by-nc-sa/4.0 Open Veterinary Journal is licensed under a Creative Commons Attribution 4.0 International License. |
spellingShingle | Original Article Nagina, G. Asima, A. Nemat, U. Shamim, A. Effect of melatonin on maturation capacity and fertilization of Nili-Ravi buffalo (Bubalus bubalis) oocytes |
title | Effect of melatonin on maturation capacity and fertilization of Nili-Ravi buffalo (Bubalus bubalis) oocytes |
title_full | Effect of melatonin on maturation capacity and fertilization of Nili-Ravi buffalo (Bubalus bubalis) oocytes |
title_fullStr | Effect of melatonin on maturation capacity and fertilization of Nili-Ravi buffalo (Bubalus bubalis) oocytes |
title_full_unstemmed | Effect of melatonin on maturation capacity and fertilization of Nili-Ravi buffalo (Bubalus bubalis) oocytes |
title_short | Effect of melatonin on maturation capacity and fertilization of Nili-Ravi buffalo (Bubalus bubalis) oocytes |
title_sort | effect of melatonin on maturation capacity and fertilization of nili-ravi buffalo (bubalus bubalis) oocytes |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980478/ https://www.ncbi.nlm.nih.gov/pubmed/27540514 http://dx.doi.org/10.4314/ovj.v6i2.9 |
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