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Overexpression of transport proteins improves the production of 5-aminovalerate from l-lysine in Escherichia coli
Bacterial transporters mediate the exchanges between intracellular and extracellular environments. Modification of transport route could be applied to speed up the metabolic reactions and promote the production of aimed compounds. Herein, lysine 2-monooxygenase (DavB) and δ-aminovaleramidase (DavA)...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980613/ https://www.ncbi.nlm.nih.gov/pubmed/27510748 http://dx.doi.org/10.1038/srep30884 |
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author | Li, Zhong Xu, Jing Jiang, Tongtong Ge, Yongsheng Liu, Pan Zhang, Manman Su, Zhiguo Gao, Chao Ma, Cuiqing Xu, Ping |
author_facet | Li, Zhong Xu, Jing Jiang, Tongtong Ge, Yongsheng Liu, Pan Zhang, Manman Su, Zhiguo Gao, Chao Ma, Cuiqing Xu, Ping |
author_sort | Li, Zhong |
collection | PubMed |
description | Bacterial transporters mediate the exchanges between intracellular and extracellular environments. Modification of transport route could be applied to speed up the metabolic reactions and promote the production of aimed compounds. Herein, lysine 2-monooxygenase (DavB) and δ-aminovaleramidase (DavA) were co-expressed in Escherichia coli BL21(DE3) to produce nylon-5 monomer 5-aminovalerate from l-lysine. Then, PP2911 (4-aminobutyrate transporter in Pseudomonas putida) and LysP (the lysine specific permease in E. coli) were overexpressed to promote 5-aminovalerate production using whole cells of recombinant E. coli. The constructed E. coli strain overexpressing transport proteins exhibited good 5-aminovalerate production performance and might serve as a promising biocatalyst for 5-aminovalerate production from l-lysine. This strategy not only shows an efficient process for the production of nylon monomers but also might be used in production of other chemicals. |
format | Online Article Text |
id | pubmed-4980613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49806132016-08-19 Overexpression of transport proteins improves the production of 5-aminovalerate from l-lysine in Escherichia coli Li, Zhong Xu, Jing Jiang, Tongtong Ge, Yongsheng Liu, Pan Zhang, Manman Su, Zhiguo Gao, Chao Ma, Cuiqing Xu, Ping Sci Rep Article Bacterial transporters mediate the exchanges between intracellular and extracellular environments. Modification of transport route could be applied to speed up the metabolic reactions and promote the production of aimed compounds. Herein, lysine 2-monooxygenase (DavB) and δ-aminovaleramidase (DavA) were co-expressed in Escherichia coli BL21(DE3) to produce nylon-5 monomer 5-aminovalerate from l-lysine. Then, PP2911 (4-aminobutyrate transporter in Pseudomonas putida) and LysP (the lysine specific permease in E. coli) were overexpressed to promote 5-aminovalerate production using whole cells of recombinant E. coli. The constructed E. coli strain overexpressing transport proteins exhibited good 5-aminovalerate production performance and might serve as a promising biocatalyst for 5-aminovalerate production from l-lysine. This strategy not only shows an efficient process for the production of nylon monomers but also might be used in production of other chemicals. Nature Publishing Group 2016-08-11 /pmc/articles/PMC4980613/ /pubmed/27510748 http://dx.doi.org/10.1038/srep30884 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Li, Zhong Xu, Jing Jiang, Tongtong Ge, Yongsheng Liu, Pan Zhang, Manman Su, Zhiguo Gao, Chao Ma, Cuiqing Xu, Ping Overexpression of transport proteins improves the production of 5-aminovalerate from l-lysine in Escherichia coli |
title | Overexpression of transport proteins improves the production of 5-aminovalerate from l-lysine in Escherichia coli |
title_full | Overexpression of transport proteins improves the production of 5-aminovalerate from l-lysine in Escherichia coli |
title_fullStr | Overexpression of transport proteins improves the production of 5-aminovalerate from l-lysine in Escherichia coli |
title_full_unstemmed | Overexpression of transport proteins improves the production of 5-aminovalerate from l-lysine in Escherichia coli |
title_short | Overexpression of transport proteins improves the production of 5-aminovalerate from l-lysine in Escherichia coli |
title_sort | overexpression of transport proteins improves the production of 5-aminovalerate from l-lysine in escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980613/ https://www.ncbi.nlm.nih.gov/pubmed/27510748 http://dx.doi.org/10.1038/srep30884 |
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