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Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes

Due to their excellent physical and chemical characteristics, multi-wall carbon nanotubes (MWCNT) have the potential to be used in structural composites, conductive materials, sensors, drug delivery and medical imaging. However, because of their small-size and light-weight, the applications of MWCNT...

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Autores principales: Chen, Zhaowei, Wang, Qixin, Asmani, Mohammadnabi, Li, Yan, Liu, Chang, Li, Changning, Lippmann, Julian M., Wu, Yun, Zhao, Ruogang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980669/
https://www.ncbi.nlm.nih.gov/pubmed/27510174
http://dx.doi.org/10.1038/srep31304
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author Chen, Zhaowei
Wang, Qixin
Asmani, Mohammadnabi
Li, Yan
Liu, Chang
Li, Changning
Lippmann, Julian M.
Wu, Yun
Zhao, Ruogang
author_facet Chen, Zhaowei
Wang, Qixin
Asmani, Mohammadnabi
Li, Yan
Liu, Chang
Li, Changning
Lippmann, Julian M.
Wu, Yun
Zhao, Ruogang
author_sort Chen, Zhaowei
collection PubMed
description Due to their excellent physical and chemical characteristics, multi-wall carbon nanotubes (MWCNT) have the potential to be used in structural composites, conductive materials, sensors, drug delivery and medical imaging. However, because of their small-size and light-weight, the applications of MWCNT also raise health concerns. In vivo animal studies have shown that MWCNT cause biomechanical and genetic alterations in the lung tissue which lead to lung fibrosis. To screen the fibrogenic risk factor of specific types of MWCNT, we developed a human lung microtissue array device that allows real-time and in-situ readout of the biomechanical properties of the engineered lung microtissue upon MWCNT insult. We showed that the higher the MWCNT concentration, the more severe cytotoxicity was observed. More importantly, short type MWCNT at low concentration of 50 ng/ml stimulated microtissue formation and contraction force generation, and caused substantial increase in the fibrogenic marker miR-21 expression, indicating the high fibrogenic potential of this specific carbon nanotube type and concentration. The presented microtissue array system provides a powerful tool for high-throughput examination of the therapeutic and toxicological effects of target compounds in realistic tissue environment.
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spelling pubmed-49806692016-08-19 Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes Chen, Zhaowei Wang, Qixin Asmani, Mohammadnabi Li, Yan Liu, Chang Li, Changning Lippmann, Julian M. Wu, Yun Zhao, Ruogang Sci Rep Article Due to their excellent physical and chemical characteristics, multi-wall carbon nanotubes (MWCNT) have the potential to be used in structural composites, conductive materials, sensors, drug delivery and medical imaging. However, because of their small-size and light-weight, the applications of MWCNT also raise health concerns. In vivo animal studies have shown that MWCNT cause biomechanical and genetic alterations in the lung tissue which lead to lung fibrosis. To screen the fibrogenic risk factor of specific types of MWCNT, we developed a human lung microtissue array device that allows real-time and in-situ readout of the biomechanical properties of the engineered lung microtissue upon MWCNT insult. We showed that the higher the MWCNT concentration, the more severe cytotoxicity was observed. More importantly, short type MWCNT at low concentration of 50 ng/ml stimulated microtissue formation and contraction force generation, and caused substantial increase in the fibrogenic marker miR-21 expression, indicating the high fibrogenic potential of this specific carbon nanotube type and concentration. The presented microtissue array system provides a powerful tool for high-throughput examination of the therapeutic and toxicological effects of target compounds in realistic tissue environment. Nature Publishing Group 2016-08-11 /pmc/articles/PMC4980669/ /pubmed/27510174 http://dx.doi.org/10.1038/srep31304 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Chen, Zhaowei
Wang, Qixin
Asmani, Mohammadnabi
Li, Yan
Liu, Chang
Li, Changning
Lippmann, Julian M.
Wu, Yun
Zhao, Ruogang
Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes
title Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes
title_full Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes
title_fullStr Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes
title_full_unstemmed Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes
title_short Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes
title_sort lung microtissue array to screen the fibrogenic potential of carbon nanotubes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980669/
https://www.ncbi.nlm.nih.gov/pubmed/27510174
http://dx.doi.org/10.1038/srep31304
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