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Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes
Due to their excellent physical and chemical characteristics, multi-wall carbon nanotubes (MWCNT) have the potential to be used in structural composites, conductive materials, sensors, drug delivery and medical imaging. However, because of their small-size and light-weight, the applications of MWCNT...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980669/ https://www.ncbi.nlm.nih.gov/pubmed/27510174 http://dx.doi.org/10.1038/srep31304 |
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author | Chen, Zhaowei Wang, Qixin Asmani, Mohammadnabi Li, Yan Liu, Chang Li, Changning Lippmann, Julian M. Wu, Yun Zhao, Ruogang |
author_facet | Chen, Zhaowei Wang, Qixin Asmani, Mohammadnabi Li, Yan Liu, Chang Li, Changning Lippmann, Julian M. Wu, Yun Zhao, Ruogang |
author_sort | Chen, Zhaowei |
collection | PubMed |
description | Due to their excellent physical and chemical characteristics, multi-wall carbon nanotubes (MWCNT) have the potential to be used in structural composites, conductive materials, sensors, drug delivery and medical imaging. However, because of their small-size and light-weight, the applications of MWCNT also raise health concerns. In vivo animal studies have shown that MWCNT cause biomechanical and genetic alterations in the lung tissue which lead to lung fibrosis. To screen the fibrogenic risk factor of specific types of MWCNT, we developed a human lung microtissue array device that allows real-time and in-situ readout of the biomechanical properties of the engineered lung microtissue upon MWCNT insult. We showed that the higher the MWCNT concentration, the more severe cytotoxicity was observed. More importantly, short type MWCNT at low concentration of 50 ng/ml stimulated microtissue formation and contraction force generation, and caused substantial increase in the fibrogenic marker miR-21 expression, indicating the high fibrogenic potential of this specific carbon nanotube type and concentration. The presented microtissue array system provides a powerful tool for high-throughput examination of the therapeutic and toxicological effects of target compounds in realistic tissue environment. |
format | Online Article Text |
id | pubmed-4980669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49806692016-08-19 Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes Chen, Zhaowei Wang, Qixin Asmani, Mohammadnabi Li, Yan Liu, Chang Li, Changning Lippmann, Julian M. Wu, Yun Zhao, Ruogang Sci Rep Article Due to their excellent physical and chemical characteristics, multi-wall carbon nanotubes (MWCNT) have the potential to be used in structural composites, conductive materials, sensors, drug delivery and medical imaging. However, because of their small-size and light-weight, the applications of MWCNT also raise health concerns. In vivo animal studies have shown that MWCNT cause biomechanical and genetic alterations in the lung tissue which lead to lung fibrosis. To screen the fibrogenic risk factor of specific types of MWCNT, we developed a human lung microtissue array device that allows real-time and in-situ readout of the biomechanical properties of the engineered lung microtissue upon MWCNT insult. We showed that the higher the MWCNT concentration, the more severe cytotoxicity was observed. More importantly, short type MWCNT at low concentration of 50 ng/ml stimulated microtissue formation and contraction force generation, and caused substantial increase in the fibrogenic marker miR-21 expression, indicating the high fibrogenic potential of this specific carbon nanotube type and concentration. The presented microtissue array system provides a powerful tool for high-throughput examination of the therapeutic and toxicological effects of target compounds in realistic tissue environment. Nature Publishing Group 2016-08-11 /pmc/articles/PMC4980669/ /pubmed/27510174 http://dx.doi.org/10.1038/srep31304 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Chen, Zhaowei Wang, Qixin Asmani, Mohammadnabi Li, Yan Liu, Chang Li, Changning Lippmann, Julian M. Wu, Yun Zhao, Ruogang Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes |
title | Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes |
title_full | Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes |
title_fullStr | Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes |
title_full_unstemmed | Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes |
title_short | Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes |
title_sort | lung microtissue array to screen the fibrogenic potential of carbon nanotubes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980669/ https://www.ncbi.nlm.nih.gov/pubmed/27510174 http://dx.doi.org/10.1038/srep31304 |
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