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A statistical approach for optimization of alkaline lipase production by ascidian associated—Halobacillus trueperi RSK CAS9
A marine ascidian-associated bacterium, Halobacillus trueperi RSK CAS9, was optimized for lipase production by response surface methodology using marine waste as substrate. The central composite design was employed, and the optimal medium constituents for maximum lipase production (1355.81 U/ml) wer...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980739/ https://www.ncbi.nlm.nih.gov/pubmed/28352574 http://dx.doi.org/10.1016/j.btre.2015.09.002 |
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author | Sathishkumar, Ramamoorthy Ananthan, Gnanakkan Iyappan, Kathirvel Stalin, Chinnathambi |
author_facet | Sathishkumar, Ramamoorthy Ananthan, Gnanakkan Iyappan, Kathirvel Stalin, Chinnathambi |
author_sort | Sathishkumar, Ramamoorthy |
collection | PubMed |
description | A marine ascidian-associated bacterium, Halobacillus trueperi RSK CAS9, was optimized for lipase production by response surface methodology using marine waste as substrate. The central composite design was employed, and the optimal medium constituents for maximum lipase production (1355.81 U/ml) were determined to be tuna powder (14.58 g/l), olive oil (5.05 ml/l); NaCl (72.42 g/l), temperature (45 °C) and pH 9.0. An alkaline lipase was purified to 8.46 fold with 1193.59 U mg(−1) specific activities with the molecular weight of 44 kDa. The activity was substantially inhibited by EDTA and PMSF, indicating that it was a metalloenzyme serine residue which was essential for catalytic activity. Thus, lipase production by microbial conversion of marine fish wastes in this study suggested its potential utilization for the production of high value products. |
format | Online Article Text |
id | pubmed-4980739 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-49807392017-03-28 A statistical approach for optimization of alkaline lipase production by ascidian associated—Halobacillus trueperi RSK CAS9 Sathishkumar, Ramamoorthy Ananthan, Gnanakkan Iyappan, Kathirvel Stalin, Chinnathambi Biotechnol Rep (Amst) Article A marine ascidian-associated bacterium, Halobacillus trueperi RSK CAS9, was optimized for lipase production by response surface methodology using marine waste as substrate. The central composite design was employed, and the optimal medium constituents for maximum lipase production (1355.81 U/ml) were determined to be tuna powder (14.58 g/l), olive oil (5.05 ml/l); NaCl (72.42 g/l), temperature (45 °C) and pH 9.0. An alkaline lipase was purified to 8.46 fold with 1193.59 U mg(−1) specific activities with the molecular weight of 44 kDa. The activity was substantially inhibited by EDTA and PMSF, indicating that it was a metalloenzyme serine residue which was essential for catalytic activity. Thus, lipase production by microbial conversion of marine fish wastes in this study suggested its potential utilization for the production of high value products. Elsevier 2015-09-16 /pmc/articles/PMC4980739/ /pubmed/28352574 http://dx.doi.org/10.1016/j.btre.2015.09.002 Text en © 2015 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Sathishkumar, Ramamoorthy Ananthan, Gnanakkan Iyappan, Kathirvel Stalin, Chinnathambi A statistical approach for optimization of alkaline lipase production by ascidian associated—Halobacillus trueperi RSK CAS9 |
title | A statistical approach for optimization of alkaline lipase production by ascidian associated—Halobacillus trueperi RSK CAS9 |
title_full | A statistical approach for optimization of alkaline lipase production by ascidian associated—Halobacillus trueperi RSK CAS9 |
title_fullStr | A statistical approach for optimization of alkaline lipase production by ascidian associated—Halobacillus trueperi RSK CAS9 |
title_full_unstemmed | A statistical approach for optimization of alkaline lipase production by ascidian associated—Halobacillus trueperi RSK CAS9 |
title_short | A statistical approach for optimization of alkaline lipase production by ascidian associated—Halobacillus trueperi RSK CAS9 |
title_sort | statistical approach for optimization of alkaline lipase production by ascidian associated—halobacillus trueperi rsk cas9 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980739/ https://www.ncbi.nlm.nih.gov/pubmed/28352574 http://dx.doi.org/10.1016/j.btre.2015.09.002 |
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