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A statistical approach for optimization of alkaline lipase production by ascidian associated—Halobacillus trueperi RSK CAS9

A marine ascidian-associated bacterium, Halobacillus trueperi RSK CAS9, was optimized for lipase production by response surface methodology using marine waste as substrate. The central composite design was employed, and the optimal medium constituents for maximum lipase production (1355.81 U/ml) wer...

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Autores principales: Sathishkumar, Ramamoorthy, Ananthan, Gnanakkan, Iyappan, Kathirvel, Stalin, Chinnathambi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980739/
https://www.ncbi.nlm.nih.gov/pubmed/28352574
http://dx.doi.org/10.1016/j.btre.2015.09.002
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author Sathishkumar, Ramamoorthy
Ananthan, Gnanakkan
Iyappan, Kathirvel
Stalin, Chinnathambi
author_facet Sathishkumar, Ramamoorthy
Ananthan, Gnanakkan
Iyappan, Kathirvel
Stalin, Chinnathambi
author_sort Sathishkumar, Ramamoorthy
collection PubMed
description A marine ascidian-associated bacterium, Halobacillus trueperi RSK CAS9, was optimized for lipase production by response surface methodology using marine waste as substrate. The central composite design was employed, and the optimal medium constituents for maximum lipase production (1355.81 U/ml) were determined to be tuna powder (14.58 g/l), olive oil (5.05 ml/l); NaCl (72.42 g/l), temperature (45 °C) and pH 9.0. An alkaline lipase was purified to 8.46 fold with 1193.59 U mg(−1) specific activities with the molecular weight of 44 kDa. The activity was substantially inhibited by EDTA and PMSF, indicating that it was a metalloenzyme serine residue which was essential for catalytic activity. Thus, lipase production by microbial conversion of marine fish wastes in this study suggested its potential utilization for the production of high value products.
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spelling pubmed-49807392017-03-28 A statistical approach for optimization of alkaline lipase production by ascidian associated—Halobacillus trueperi RSK CAS9 Sathishkumar, Ramamoorthy Ananthan, Gnanakkan Iyappan, Kathirvel Stalin, Chinnathambi Biotechnol Rep (Amst) Article A marine ascidian-associated bacterium, Halobacillus trueperi RSK CAS9, was optimized for lipase production by response surface methodology using marine waste as substrate. The central composite design was employed, and the optimal medium constituents for maximum lipase production (1355.81 U/ml) were determined to be tuna powder (14.58 g/l), olive oil (5.05 ml/l); NaCl (72.42 g/l), temperature (45 °C) and pH 9.0. An alkaline lipase was purified to 8.46 fold with 1193.59 U mg(−1) specific activities with the molecular weight of 44 kDa. The activity was substantially inhibited by EDTA and PMSF, indicating that it was a metalloenzyme serine residue which was essential for catalytic activity. Thus, lipase production by microbial conversion of marine fish wastes in this study suggested its potential utilization for the production of high value products. Elsevier 2015-09-16 /pmc/articles/PMC4980739/ /pubmed/28352574 http://dx.doi.org/10.1016/j.btre.2015.09.002 Text en © 2015 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Sathishkumar, Ramamoorthy
Ananthan, Gnanakkan
Iyappan, Kathirvel
Stalin, Chinnathambi
A statistical approach for optimization of alkaline lipase production by ascidian associated—Halobacillus trueperi RSK CAS9
title A statistical approach for optimization of alkaline lipase production by ascidian associated—Halobacillus trueperi RSK CAS9
title_full A statistical approach for optimization of alkaline lipase production by ascidian associated—Halobacillus trueperi RSK CAS9
title_fullStr A statistical approach for optimization of alkaline lipase production by ascidian associated—Halobacillus trueperi RSK CAS9
title_full_unstemmed A statistical approach for optimization of alkaline lipase production by ascidian associated—Halobacillus trueperi RSK CAS9
title_short A statistical approach for optimization of alkaline lipase production by ascidian associated—Halobacillus trueperi RSK CAS9
title_sort statistical approach for optimization of alkaline lipase production by ascidian associated—halobacillus trueperi rsk cas9
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980739/
https://www.ncbi.nlm.nih.gov/pubmed/28352574
http://dx.doi.org/10.1016/j.btre.2015.09.002
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