Cargando…

Proteomic analysis of an engineered isolate of Lactobacillus plantarum with enhanced raffinose metabolic capacity

Lactic acid bacteria that can produce alpha-galactosidase are a promising solution for improving the nutritional value of soy-derived products. For their commercial use in the manufacturing process, it is essential to understand the catabolic mechanisms that facilitate their growth and performance....

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Jicheng, Hui, Wenyan, Cao, Chenxia, Jin, Rulin, Ren, Caixia, Zhang, Heping, Zhang, Wenyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980766/
https://www.ncbi.nlm.nih.gov/pubmed/27510766
http://dx.doi.org/10.1038/srep31403
_version_ 1782447511096524800
author Wang, Jicheng
Hui, Wenyan
Cao, Chenxia
Jin, Rulin
Ren, Caixia
Zhang, Heping
Zhang, Wenyi
author_facet Wang, Jicheng
Hui, Wenyan
Cao, Chenxia
Jin, Rulin
Ren, Caixia
Zhang, Heping
Zhang, Wenyi
author_sort Wang, Jicheng
collection PubMed
description Lactic acid bacteria that can produce alpha-galactosidase are a promising solution for improving the nutritional value of soy-derived products. For their commercial use in the manufacturing process, it is essential to understand the catabolic mechanisms that facilitate their growth and performance. In this study, we used comparative proteomic analysis to compare catabolism in an engineered isolate of Lactobacillus plantarum P-8 with enhanced raffinose metabolic capacity, with the parent (or wild-type) isolate from which it was derived. When growing on semi-defined medium with raffinose, a total of one hundred and twenty-five proteins were significantly up-regulated (>1.5 fold, P < 0.05) in the engineered isolate, whilst and one hundred and six proteins were significantly down-regulated (<−1.5 fold, P < 0.05). During the late stages of growth, the engineered isolate was able to utilise alternative carbohydrates such as sorbitol instead of raffinose to sustain cell division. To avoid acid damage the cell layer of the engineered isolate altered through a combination of de novo fatty acid biosynthesis and modification of existing lipid membrane phospholipid acyl chains. Interestingly, aspartate and glutamate metabolism was associated with this acid response. Higher intracellular aspartate and glutamate levels in the engineered isolate compared with the parent isolate were confirmed by further chemical analysis. Our study will underpin the future use of this engineered isolate in the manufacture of soymilk products.
format Online
Article
Text
id pubmed-4980766
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-49807662016-08-19 Proteomic analysis of an engineered isolate of Lactobacillus plantarum with enhanced raffinose metabolic capacity Wang, Jicheng Hui, Wenyan Cao, Chenxia Jin, Rulin Ren, Caixia Zhang, Heping Zhang, Wenyi Sci Rep Article Lactic acid bacteria that can produce alpha-galactosidase are a promising solution for improving the nutritional value of soy-derived products. For their commercial use in the manufacturing process, it is essential to understand the catabolic mechanisms that facilitate their growth and performance. In this study, we used comparative proteomic analysis to compare catabolism in an engineered isolate of Lactobacillus plantarum P-8 with enhanced raffinose metabolic capacity, with the parent (or wild-type) isolate from which it was derived. When growing on semi-defined medium with raffinose, a total of one hundred and twenty-five proteins were significantly up-regulated (>1.5 fold, P < 0.05) in the engineered isolate, whilst and one hundred and six proteins were significantly down-regulated (<−1.5 fold, P < 0.05). During the late stages of growth, the engineered isolate was able to utilise alternative carbohydrates such as sorbitol instead of raffinose to sustain cell division. To avoid acid damage the cell layer of the engineered isolate altered through a combination of de novo fatty acid biosynthesis and modification of existing lipid membrane phospholipid acyl chains. Interestingly, aspartate and glutamate metabolism was associated with this acid response. Higher intracellular aspartate and glutamate levels in the engineered isolate compared with the parent isolate were confirmed by further chemical analysis. Our study will underpin the future use of this engineered isolate in the manufacture of soymilk products. Nature Publishing Group 2016-08-11 /pmc/articles/PMC4980766/ /pubmed/27510766 http://dx.doi.org/10.1038/srep31403 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wang, Jicheng
Hui, Wenyan
Cao, Chenxia
Jin, Rulin
Ren, Caixia
Zhang, Heping
Zhang, Wenyi
Proteomic analysis of an engineered isolate of Lactobacillus plantarum with enhanced raffinose metabolic capacity
title Proteomic analysis of an engineered isolate of Lactobacillus plantarum with enhanced raffinose metabolic capacity
title_full Proteomic analysis of an engineered isolate of Lactobacillus plantarum with enhanced raffinose metabolic capacity
title_fullStr Proteomic analysis of an engineered isolate of Lactobacillus plantarum with enhanced raffinose metabolic capacity
title_full_unstemmed Proteomic analysis of an engineered isolate of Lactobacillus plantarum with enhanced raffinose metabolic capacity
title_short Proteomic analysis of an engineered isolate of Lactobacillus plantarum with enhanced raffinose metabolic capacity
title_sort proteomic analysis of an engineered isolate of lactobacillus plantarum with enhanced raffinose metabolic capacity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980766/
https://www.ncbi.nlm.nih.gov/pubmed/27510766
http://dx.doi.org/10.1038/srep31403
work_keys_str_mv AT wangjicheng proteomicanalysisofanengineeredisolateoflactobacillusplantarumwithenhancedraffinosemetaboliccapacity
AT huiwenyan proteomicanalysisofanengineeredisolateoflactobacillusplantarumwithenhancedraffinosemetaboliccapacity
AT caochenxia proteomicanalysisofanengineeredisolateoflactobacillusplantarumwithenhancedraffinosemetaboliccapacity
AT jinrulin proteomicanalysisofanengineeredisolateoflactobacillusplantarumwithenhancedraffinosemetaboliccapacity
AT rencaixia proteomicanalysisofanengineeredisolateoflactobacillusplantarumwithenhancedraffinosemetaboliccapacity
AT zhangheping proteomicanalysisofanengineeredisolateoflactobacillusplantarumwithenhancedraffinosemetaboliccapacity
AT zhangwenyi proteomicanalysisofanengineeredisolateoflactobacillusplantarumwithenhancedraffinosemetaboliccapacity