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Stability of bovine coronavirus on lettuce surfaces under household refrigeration conditions
Fecal suspensions with an aerosol route of transmission were responsible for a cluster of severe acute respiratory syndrome (SARS) cases in 2003 in Hong Kong. Based on that event, the World Health Organization recommended that research be implemented to define modes of transmission of SARS coronavir...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980993/ https://www.ncbi.nlm.nih.gov/pubmed/22265299 http://dx.doi.org/10.1016/j.fm.2011.12.009 |
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author | Mullis, Lisa Saif, Linda J. Zhang, Yongbin Zhang, Xuming Azevedo, Marli S.P. |
author_facet | Mullis, Lisa Saif, Linda J. Zhang, Yongbin Zhang, Xuming Azevedo, Marli S.P. |
author_sort | Mullis, Lisa |
collection | PubMed |
description | Fecal suspensions with an aerosol route of transmission were responsible for a cluster of severe acute respiratory syndrome (SARS) cases in 2003 in Hong Kong. Based on that event, the World Health Organization recommended that research be implemented to define modes of transmission of SARS coronavirus through sewage, feces, food and water. Environmental studies have shown that animal coronaviruses remain infectious in water and sewage for up to a year depending on the temperature and humidity. In this study, we examined coronavirus stability on lettuce surfaces. A cell culture adapted bovine coronavirus, diluted in growth media or in bovine fecal suspensions to simulate fecal contamination was used to spike romaine lettuce. qRT-PCR detected viral RNA copy number ranging from 6.6 × 10(4) to 1.7 × 10(6) throughout the experimental period of 30 days. Whereas infectious viruses were detected for at least 14 days, the amount of infectious virus varied, depending upon the diluent used for spiking the lettuce. UV and confocal microscopic observation indicated attachment of residual labeled virions to the lettuce surface after the elution procedure, suggesting that rates of inactivation or detection of the virus may be underestimated. Thus, it is possible that contaminated vegetables may be potential vehicles for coronavirus zoonotic transmission to humans. |
format | Online Article Text |
id | pubmed-4980993 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-49809932016-08-11 Stability of bovine coronavirus on lettuce surfaces under household refrigeration conditions Mullis, Lisa Saif, Linda J. Zhang, Yongbin Zhang, Xuming Azevedo, Marli S.P. Food Microbiol Article Fecal suspensions with an aerosol route of transmission were responsible for a cluster of severe acute respiratory syndrome (SARS) cases in 2003 in Hong Kong. Based on that event, the World Health Organization recommended that research be implemented to define modes of transmission of SARS coronavirus through sewage, feces, food and water. Environmental studies have shown that animal coronaviruses remain infectious in water and sewage for up to a year depending on the temperature and humidity. In this study, we examined coronavirus stability on lettuce surfaces. A cell culture adapted bovine coronavirus, diluted in growth media or in bovine fecal suspensions to simulate fecal contamination was used to spike romaine lettuce. qRT-PCR detected viral RNA copy number ranging from 6.6 × 10(4) to 1.7 × 10(6) throughout the experimental period of 30 days. Whereas infectious viruses were detected for at least 14 days, the amount of infectious virus varied, depending upon the diluent used for spiking the lettuce. UV and confocal microscopic observation indicated attachment of residual labeled virions to the lettuce surface after the elution procedure, suggesting that rates of inactivation or detection of the virus may be underestimated. Thus, it is possible that contaminated vegetables may be potential vehicles for coronavirus zoonotic transmission to humans. Elsevier 2012-05 2011-12-13 /pmc/articles/PMC4980993/ /pubmed/22265299 http://dx.doi.org/10.1016/j.fm.2011.12.009 Text en Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Mullis, Lisa Saif, Linda J. Zhang, Yongbin Zhang, Xuming Azevedo, Marli S.P. Stability of bovine coronavirus on lettuce surfaces under household refrigeration conditions |
title | Stability of bovine coronavirus on lettuce surfaces under household refrigeration conditions |
title_full | Stability of bovine coronavirus on lettuce surfaces under household refrigeration conditions |
title_fullStr | Stability of bovine coronavirus on lettuce surfaces under household refrigeration conditions |
title_full_unstemmed | Stability of bovine coronavirus on lettuce surfaces under household refrigeration conditions |
title_short | Stability of bovine coronavirus on lettuce surfaces under household refrigeration conditions |
title_sort | stability of bovine coronavirus on lettuce surfaces under household refrigeration conditions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980993/ https://www.ncbi.nlm.nih.gov/pubmed/22265299 http://dx.doi.org/10.1016/j.fm.2011.12.009 |
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