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Combating a Global Threat to a Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) Genomes Reveal Clues for Disease Control
Black Sigatoka or black leaf streak disease, caused by the Dothideomycete fungus Pseudocercospora fijiensis (previously: Mycosphaerella fijiensis), is the most significant foliar disease of banana worldwide. Due to the lack of effective host resistance, management of this disease requires frequent f...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4981457/ https://www.ncbi.nlm.nih.gov/pubmed/27512984 http://dx.doi.org/10.1371/journal.pgen.1005876 |
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author | Arango Isaza, Rafael E. Diaz-Trujillo, Caucasella Dhillon, Braham Aerts, Andrea Carlier, Jean Crane, Charles F. V. de Jong, Tristan de Vries, Ineke Dietrich, Robert Farmer, Andrew D. Fortes Fereira, Claudia Garcia, Suzana Guzman, Mauricio Hamelin, Richard C. Lindquist, Erika A. Mehrabi, Rahim Quiros, Olman Schmutz, Jeremy Shapiro, Harris Reynolds, Elizabeth Scalliet, Gabriel Souza, Manoel Stergiopoulos, Ioannis Van der Lee, Theo A. J. De Wit, Pierre J. G. M. Zapater, Marie-Françoise Zwiers, Lute-Harm Grigoriev, Igor V. Goodwin, Stephen B. Kema, Gert H. J. |
author_facet | Arango Isaza, Rafael E. Diaz-Trujillo, Caucasella Dhillon, Braham Aerts, Andrea Carlier, Jean Crane, Charles F. V. de Jong, Tristan de Vries, Ineke Dietrich, Robert Farmer, Andrew D. Fortes Fereira, Claudia Garcia, Suzana Guzman, Mauricio Hamelin, Richard C. Lindquist, Erika A. Mehrabi, Rahim Quiros, Olman Schmutz, Jeremy Shapiro, Harris Reynolds, Elizabeth Scalliet, Gabriel Souza, Manoel Stergiopoulos, Ioannis Van der Lee, Theo A. J. De Wit, Pierre J. G. M. Zapater, Marie-Françoise Zwiers, Lute-Harm Grigoriev, Igor V. Goodwin, Stephen B. Kema, Gert H. J. |
author_sort | Arango Isaza, Rafael E. |
collection | PubMed |
description | Black Sigatoka or black leaf streak disease, caused by the Dothideomycete fungus Pseudocercospora fijiensis (previously: Mycosphaerella fijiensis), is the most significant foliar disease of banana worldwide. Due to the lack of effective host resistance, management of this disease requires frequent fungicide applications, which greatly increase the economic and environmental costs to produce banana. Weekly applications in most banana plantations lead to rapid evolution of fungicide-resistant strains within populations causing disease-control failures throughout the world. Given its extremely high economic importance, two strains of P. fijiensis were sequenced and assembled with the aid of a new genetic linkage map. The 74-Mb genome of P. fijiensis is massively expanded by LTR retrotransposons, making it the largest genome within the Dothideomycetes. Melting-curve assays suggest that the genomes of two closely related members of the Sigatoka disease complex, P. eumusae and P. musae, also are expanded. Electrophoretic karyotyping and analyses of molecular markers in P. fijiensis field populations showed chromosome-length polymorphisms and high genetic diversity. Genetic differentiation was also detected using neutral markers, suggesting strong selection with limited gene flow at the studied geographic scale. Frequencies of fungicide resistance in fungicide-treated plantations were much higher than those in untreated wild-type P. fijiensis populations. A homologue of the Cladosporium fulvum Avr4 effector, PfAvr4, was identified in the P. fijiensis genome. Infiltration of the purified PfAVR4 protein into leaves of the resistant banana variety Calcutta 4 resulted in a hypersensitive-like response. This result suggests that Calcutta 4 could carry an unknown resistance gene recognizing PfAVR4. Besides adding to our understanding of the overall Dothideomycete genome structures, the P. fijiensis genome will aid in developing fungicide treatment schedules to combat this pathogen and in improving the efficiency of banana breeding programs. |
format | Online Article Text |
id | pubmed-4981457 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-49814572016-08-29 Combating a Global Threat to a Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) Genomes Reveal Clues for Disease Control Arango Isaza, Rafael E. Diaz-Trujillo, Caucasella Dhillon, Braham Aerts, Andrea Carlier, Jean Crane, Charles F. V. de Jong, Tristan de Vries, Ineke Dietrich, Robert Farmer, Andrew D. Fortes Fereira, Claudia Garcia, Suzana Guzman, Mauricio Hamelin, Richard C. Lindquist, Erika A. Mehrabi, Rahim Quiros, Olman Schmutz, Jeremy Shapiro, Harris Reynolds, Elizabeth Scalliet, Gabriel Souza, Manoel Stergiopoulos, Ioannis Van der Lee, Theo A. J. De Wit, Pierre J. G. M. Zapater, Marie-Françoise Zwiers, Lute-Harm Grigoriev, Igor V. Goodwin, Stephen B. Kema, Gert H. J. PLoS Genet Research Article Black Sigatoka or black leaf streak disease, caused by the Dothideomycete fungus Pseudocercospora fijiensis (previously: Mycosphaerella fijiensis), is the most significant foliar disease of banana worldwide. Due to the lack of effective host resistance, management of this disease requires frequent fungicide applications, which greatly increase the economic and environmental costs to produce banana. Weekly applications in most banana plantations lead to rapid evolution of fungicide-resistant strains within populations causing disease-control failures throughout the world. Given its extremely high economic importance, two strains of P. fijiensis were sequenced and assembled with the aid of a new genetic linkage map. The 74-Mb genome of P. fijiensis is massively expanded by LTR retrotransposons, making it the largest genome within the Dothideomycetes. Melting-curve assays suggest that the genomes of two closely related members of the Sigatoka disease complex, P. eumusae and P. musae, also are expanded. Electrophoretic karyotyping and analyses of molecular markers in P. fijiensis field populations showed chromosome-length polymorphisms and high genetic diversity. Genetic differentiation was also detected using neutral markers, suggesting strong selection with limited gene flow at the studied geographic scale. Frequencies of fungicide resistance in fungicide-treated plantations were much higher than those in untreated wild-type P. fijiensis populations. A homologue of the Cladosporium fulvum Avr4 effector, PfAvr4, was identified in the P. fijiensis genome. Infiltration of the purified PfAVR4 protein into leaves of the resistant banana variety Calcutta 4 resulted in a hypersensitive-like response. This result suggests that Calcutta 4 could carry an unknown resistance gene recognizing PfAVR4. Besides adding to our understanding of the overall Dothideomycete genome structures, the P. fijiensis genome will aid in developing fungicide treatment schedules to combat this pathogen and in improving the efficiency of banana breeding programs. Public Library of Science 2016-08-11 /pmc/articles/PMC4981457/ /pubmed/27512984 http://dx.doi.org/10.1371/journal.pgen.1005876 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Arango Isaza, Rafael E. Diaz-Trujillo, Caucasella Dhillon, Braham Aerts, Andrea Carlier, Jean Crane, Charles F. V. de Jong, Tristan de Vries, Ineke Dietrich, Robert Farmer, Andrew D. Fortes Fereira, Claudia Garcia, Suzana Guzman, Mauricio Hamelin, Richard C. Lindquist, Erika A. Mehrabi, Rahim Quiros, Olman Schmutz, Jeremy Shapiro, Harris Reynolds, Elizabeth Scalliet, Gabriel Souza, Manoel Stergiopoulos, Ioannis Van der Lee, Theo A. J. De Wit, Pierre J. G. M. Zapater, Marie-Françoise Zwiers, Lute-Harm Grigoriev, Igor V. Goodwin, Stephen B. Kema, Gert H. J. Combating a Global Threat to a Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) Genomes Reveal Clues for Disease Control |
title | Combating a Global Threat to a Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) Genomes Reveal Clues for Disease Control |
title_full | Combating a Global Threat to a Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) Genomes Reveal Clues for Disease Control |
title_fullStr | Combating a Global Threat to a Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) Genomes Reveal Clues for Disease Control |
title_full_unstemmed | Combating a Global Threat to a Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) Genomes Reveal Clues for Disease Control |
title_short | Combating a Global Threat to a Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) Genomes Reveal Clues for Disease Control |
title_sort | combating a global threat to a clonal crop: banana black sigatoka pathogen pseudocercospora fijiensis (synonym mycosphaerella fijiensis) genomes reveal clues for disease control |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4981457/ https://www.ncbi.nlm.nih.gov/pubmed/27512984 http://dx.doi.org/10.1371/journal.pgen.1005876 |
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