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Fitness effects of new mutations in Chlamydomonas reinhardtii across two stress gradients
Most spontaneous mutations affecting fitness are likely to be deleterious, but the strength of selection acting on them might be impacted by environmental stress. Such stress‐dependent selection could expose hidden genetic variation, which in turn might increase the adaptive potential of stressed po...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982031/ https://www.ncbi.nlm.nih.gov/pubmed/26663473 http://dx.doi.org/10.1111/jeb.12807 |
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author | Kraemer, S. A. Morgan, A. D. Ness, R. W. Keightley, P. D. Colegrave, N. |
author_facet | Kraemer, S. A. Morgan, A. D. Ness, R. W. Keightley, P. D. Colegrave, N. |
author_sort | Kraemer, S. A. |
collection | PubMed |
description | Most spontaneous mutations affecting fitness are likely to be deleterious, but the strength of selection acting on them might be impacted by environmental stress. Such stress‐dependent selection could expose hidden genetic variation, which in turn might increase the adaptive potential of stressed populations. On the other hand, this variation might represent a genetic load and thus lead to population extinction under stress. Previous studies to determine the link between stress and mutational effects on fitness, however, have produced inconsistent results. Here, we determined the net change in fitness in 29 genotypes of the green algae Chlamydomonas reinhardtii that accumulated mutations in the near absence of selection for approximately 1000 generations across two stress gradients, increasing NaCl and decreasing phosphate. We found mutational effects to be magnified under extremely stressful conditions, but such effects were specific both to the type of stress and to the genetic background. The detection of stress‐dependent fitness effects of mutations depended on accurately scaling relative fitness measures by generation times, thus offering an explanation for the inconsistencies among previous studies. |
format | Online Article Text |
id | pubmed-4982031 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-49820312016-08-26 Fitness effects of new mutations in Chlamydomonas reinhardtii across two stress gradients Kraemer, S. A. Morgan, A. D. Ness, R. W. Keightley, P. D. Colegrave, N. J Evol Biol Research Papers Most spontaneous mutations affecting fitness are likely to be deleterious, but the strength of selection acting on them might be impacted by environmental stress. Such stress‐dependent selection could expose hidden genetic variation, which in turn might increase the adaptive potential of stressed populations. On the other hand, this variation might represent a genetic load and thus lead to population extinction under stress. Previous studies to determine the link between stress and mutational effects on fitness, however, have produced inconsistent results. Here, we determined the net change in fitness in 29 genotypes of the green algae Chlamydomonas reinhardtii that accumulated mutations in the near absence of selection for approximately 1000 generations across two stress gradients, increasing NaCl and decreasing phosphate. We found mutational effects to be magnified under extremely stressful conditions, but such effects were specific both to the type of stress and to the genetic background. The detection of stress‐dependent fitness effects of mutations depended on accurately scaling relative fitness measures by generation times, thus offering an explanation for the inconsistencies among previous studies. John Wiley and Sons Inc. 2016-01-05 2016-03 /pmc/articles/PMC4982031/ /pubmed/26663473 http://dx.doi.org/10.1111/jeb.12807 Text en © 2015 The Authors. Journal of Evolutionary Biology published by John Wiley & Sons Ltd on behalf of European Society for Evolutionary Biology. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Kraemer, S. A. Morgan, A. D. Ness, R. W. Keightley, P. D. Colegrave, N. Fitness effects of new mutations in Chlamydomonas reinhardtii across two stress gradients |
title | Fitness effects of new mutations in Chlamydomonas reinhardtii across two stress gradients |
title_full | Fitness effects of new mutations in Chlamydomonas reinhardtii across two stress gradients |
title_fullStr | Fitness effects of new mutations in Chlamydomonas reinhardtii across two stress gradients |
title_full_unstemmed | Fitness effects of new mutations in Chlamydomonas reinhardtii across two stress gradients |
title_short | Fitness effects of new mutations in Chlamydomonas reinhardtii across two stress gradients |
title_sort | fitness effects of new mutations in chlamydomonas reinhardtii across two stress gradients |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982031/ https://www.ncbi.nlm.nih.gov/pubmed/26663473 http://dx.doi.org/10.1111/jeb.12807 |
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