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Combining 3D human in vitro methods for a 3Rs evaluation of novel titanium surfaces in orthopaedic applications

In this study, we report on a group of complementary human osteoblast in vitro test methods for the preclinical evaluation of 3D porous titanium surfaces. The surfaces were prepared by additive manufacturing (electron beam melting [EBM]) and plasma spraying, allowing the creation of complex lattice...

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Autores principales: Stevenson, G., Rehman, S., Draper, E., Hernández‐Nava, E., Hunt, J., Haycock, J.W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982034/
https://www.ncbi.nlm.nih.gov/pubmed/26702609
http://dx.doi.org/10.1002/bit.25919
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author Stevenson, G.
Rehman, S.
Draper, E.
Hernández‐Nava, E.
Hunt, J.
Haycock, J.W.
author_facet Stevenson, G.
Rehman, S.
Draper, E.
Hernández‐Nava, E.
Hunt, J.
Haycock, J.W.
author_sort Stevenson, G.
collection PubMed
description In this study, we report on a group of complementary human osteoblast in vitro test methods for the preclinical evaluation of 3D porous titanium surfaces. The surfaces were prepared by additive manufacturing (electron beam melting [EBM]) and plasma spraying, allowing the creation of complex lattice surface geometries. Physical properties of the surfaces were characterized by SEM and profilometry and 3D in vitro cell culture using human osteoblasts. Primary human osteoblast cells were found to elicit greater differences between titanium sample surfaces than an MG63 osteoblast‐like cell line, particularly in terms of cell survival. Surface morphology was associated with higher osteoblast metabolic activity and mineralization on rougher titanium plasma spray coated surfaces than smoother surfaces. Differences in osteoblast survival and metabolic activity on titanium lattice structures were also found, despite analogous surface morphology at the cellular level. 3D confocal microscopy identified osteoblast organization within complex titanium surface geometries, adhesion, spreading, and alignment to the biomaterial strut geometries. Mineralized nodule formation throughout the lattice structures was also observed, and indicative of early markers of bone in‐growth on such materials. Testing methods such as those presented are not traditionally considered by medical device manufacturers, but we suggest have value as an increasingly vital tool in efficiently translating pre‐clinical studies, especially in balance with current regulatory practice, commercial demands, the 3Rs, and the relative merits of in vitro and in vivo studies. Biotechnol. Bioeng. 2016;113: 1586–1599. © 2015 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.
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spelling pubmed-49820342016-08-26 Combining 3D human in vitro methods for a 3Rs evaluation of novel titanium surfaces in orthopaedic applications Stevenson, G. Rehman, S. Draper, E. Hernández‐Nava, E. Hunt, J. Haycock, J.W. Biotechnol Bioeng Articles In this study, we report on a group of complementary human osteoblast in vitro test methods for the preclinical evaluation of 3D porous titanium surfaces. The surfaces were prepared by additive manufacturing (electron beam melting [EBM]) and plasma spraying, allowing the creation of complex lattice surface geometries. Physical properties of the surfaces were characterized by SEM and profilometry and 3D in vitro cell culture using human osteoblasts. Primary human osteoblast cells were found to elicit greater differences between titanium sample surfaces than an MG63 osteoblast‐like cell line, particularly in terms of cell survival. Surface morphology was associated with higher osteoblast metabolic activity and mineralization on rougher titanium plasma spray coated surfaces than smoother surfaces. Differences in osteoblast survival and metabolic activity on titanium lattice structures were also found, despite analogous surface morphology at the cellular level. 3D confocal microscopy identified osteoblast organization within complex titanium surface geometries, adhesion, spreading, and alignment to the biomaterial strut geometries. Mineralized nodule formation throughout the lattice structures was also observed, and indicative of early markers of bone in‐growth on such materials. Testing methods such as those presented are not traditionally considered by medical device manufacturers, but we suggest have value as an increasingly vital tool in efficiently translating pre‐clinical studies, especially in balance with current regulatory practice, commercial demands, the 3Rs, and the relative merits of in vitro and in vivo studies. Biotechnol. Bioeng. 2016;113: 1586–1599. © 2015 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc. John Wiley and Sons Inc. 2016-01-21 2016-07 /pmc/articles/PMC4982034/ /pubmed/26702609 http://dx.doi.org/10.1002/bit.25919 Text en © 2015 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Stevenson, G.
Rehman, S.
Draper, E.
Hernández‐Nava, E.
Hunt, J.
Haycock, J.W.
Combining 3D human in vitro methods for a 3Rs evaluation of novel titanium surfaces in orthopaedic applications
title Combining 3D human in vitro methods for a 3Rs evaluation of novel titanium surfaces in orthopaedic applications
title_full Combining 3D human in vitro methods for a 3Rs evaluation of novel titanium surfaces in orthopaedic applications
title_fullStr Combining 3D human in vitro methods for a 3Rs evaluation of novel titanium surfaces in orthopaedic applications
title_full_unstemmed Combining 3D human in vitro methods for a 3Rs evaluation of novel titanium surfaces in orthopaedic applications
title_short Combining 3D human in vitro methods for a 3Rs evaluation of novel titanium surfaces in orthopaedic applications
title_sort combining 3d human in vitro methods for a 3rs evaluation of novel titanium surfaces in orthopaedic applications
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982034/
https://www.ncbi.nlm.nih.gov/pubmed/26702609
http://dx.doi.org/10.1002/bit.25919
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