Cerebrospinal fluid real‐time quaking‐induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: An international study

Real‐time quaking‐induced conversion (RT‐QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt–Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be demonstrated. Two international ring trials were...

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Detalles Bibliográficos
Autores principales: McGuire, Lynne I., Poleggi, Anna, Poggiolini, Ilaria, Suardi, Silvia, Grznarova, Katarina, Shi, Song, de Vil, Bart, Sarros, Shannon, Satoh, Katsuya, Cheng, Keding, Cramm, Maria, Fairfoul, Graham, Schmitz, Matthias, Zerr, Inga, Cras, Patrick, Equestre, Michele, Tagliavini, Fabrizio, Atarashi, Ryuichiro, Knox, David, Collins, Steven, Haïk, Stéphane, Parchi, Piero, Pocchiari, Maurizio, Green, Alison
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Brief Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982084/
https://www.ncbi.nlm.nih.gov/pubmed/27130376
http://dx.doi.org/10.1002/ana.24679
Descripción
Sumario:Real‐time quaking‐induced conversion (RT‐QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt–Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be demonstrated. Two international ring trials were undertaken in which a set of 25 cerebrospinal fluid samples were analyzed by a total of 11 different centers using a range of recombinant prion protein substrates and instrumentation. The results show almost complete concordance between the centers and demonstrate that RT‐QuIC is a suitably reliable and robust technique for clinical practice. Ann Neurol 2016;80:160–165

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