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Cerebrospinal fluid real‐time quaking‐induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: An international study
Real‐time quaking‐induced conversion (RT‐QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt–Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be demonstrated. Two international ring trials were...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982084/ https://www.ncbi.nlm.nih.gov/pubmed/27130376 http://dx.doi.org/10.1002/ana.24679 |
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| author | McGuire, Lynne I. Poleggi, Anna Poggiolini, Ilaria Suardi, Silvia Grznarova, Katarina Shi, Song de Vil, Bart Sarros, Shannon Satoh, Katsuya Cheng, Keding Cramm, Maria Fairfoul, Graham Schmitz, Matthias Zerr, Inga Cras, Patrick Equestre, Michele Tagliavini, Fabrizio Atarashi, Ryuichiro Knox, David Collins, Steven Haïk, Stéphane Parchi, Piero Pocchiari, Maurizio Green, Alison |
| author_facet | McGuire, Lynne I. Poleggi, Anna Poggiolini, Ilaria Suardi, Silvia Grznarova, Katarina Shi, Song de Vil, Bart Sarros, Shannon Satoh, Katsuya Cheng, Keding Cramm, Maria Fairfoul, Graham Schmitz, Matthias Zerr, Inga Cras, Patrick Equestre, Michele Tagliavini, Fabrizio Atarashi, Ryuichiro Knox, David Collins, Steven Haïk, Stéphane Parchi, Piero Pocchiari, Maurizio Green, Alison |
| author_sort | McGuire, Lynne I. |
| collection | PubMed |
| description | Real‐time quaking‐induced conversion (RT‐QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt–Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be demonstrated. Two international ring trials were undertaken in which a set of 25 cerebrospinal fluid samples were analyzed by a total of 11 different centers using a range of recombinant prion protein substrates and instrumentation. The results show almost complete concordance between the centers and demonstrate that RT‐QuIC is a suitably reliable and robust technique for clinical practice. Ann Neurol 2016;80:160–165 |
| format | Online Article Text |
| id | pubmed-4982084 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2016 |
| publisher | John Wiley and Sons Inc. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-49820842016-08-24 Cerebrospinal fluid real‐time quaking‐induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: An international study McGuire, Lynne I. Poleggi, Anna Poggiolini, Ilaria Suardi, Silvia Grznarova, Katarina Shi, Song de Vil, Bart Sarros, Shannon Satoh, Katsuya Cheng, Keding Cramm, Maria Fairfoul, Graham Schmitz, Matthias Zerr, Inga Cras, Patrick Equestre, Michele Tagliavini, Fabrizio Atarashi, Ryuichiro Knox, David Collins, Steven Haïk, Stéphane Parchi, Piero Pocchiari, Maurizio Green, Alison Ann Neurol Brief Communications Real‐time quaking‐induced conversion (RT‐QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt–Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be demonstrated. Two international ring trials were undertaken in which a set of 25 cerebrospinal fluid samples were analyzed by a total of 11 different centers using a range of recombinant prion protein substrates and instrumentation. The results show almost complete concordance between the centers and demonstrate that RT‐QuIC is a suitably reliable and robust technique for clinical practice. Ann Neurol 2016;80:160–165 John Wiley and Sons Inc. 2016-06-01 2016-07 /pmc/articles/PMC4982084/ /pubmed/27130376 http://dx.doi.org/10.1002/ana.24679 Text en © 2016 The Authors. Annals of Neurology published by Wiley Periodicals, Inc. on behalf of American Neurological Association. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Brief Communications McGuire, Lynne I. Poleggi, Anna Poggiolini, Ilaria Suardi, Silvia Grznarova, Katarina Shi, Song de Vil, Bart Sarros, Shannon Satoh, Katsuya Cheng, Keding Cramm, Maria Fairfoul, Graham Schmitz, Matthias Zerr, Inga Cras, Patrick Equestre, Michele Tagliavini, Fabrizio Atarashi, Ryuichiro Knox, David Collins, Steven Haïk, Stéphane Parchi, Piero Pocchiari, Maurizio Green, Alison Cerebrospinal fluid real‐time quaking‐induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: An international study |
| title | Cerebrospinal fluid real‐time quaking‐induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: An international study |
| title_full | Cerebrospinal fluid real‐time quaking‐induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: An international study |
| title_fullStr | Cerebrospinal fluid real‐time quaking‐induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: An international study |
| title_full_unstemmed | Cerebrospinal fluid real‐time quaking‐induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: An international study |
| title_short | Cerebrospinal fluid real‐time quaking‐induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: An international study |
| title_sort | cerebrospinal fluid real‐time quaking‐induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: an international study |
| topic | Brief Communications |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982084/ https://www.ncbi.nlm.nih.gov/pubmed/27130376 http://dx.doi.org/10.1002/ana.24679 |
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