Use of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses

Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis virus and Ebola virus in...

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Detalles Bibliográficos
Autores principales: Kilianski, Andy, Roth, Pierce A., Liem, Alvin T., Hill, Jessica M., Willis, Kristen L., Rossmaier, Rebecca D., Marinich, Andrew V., Maughan, Michele N., Karavis, Mark A., Kuhn, Jens H., Honko, Anna N., Rosenzweig, C. Nicole
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2016
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982148/
https://www.ncbi.nlm.nih.gov/pubmed/27191483
http://dx.doi.org/10.3201/eid2208.160270
Descripción
Sumario:Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis virus and Ebola virus in 3 hours from sample receipt to data acquisition, demonstrating a fieldable technique for RNA virus characterization.

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