Use of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses

Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis virus and Ebola virus in...

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Autores principales: Kilianski, Andy, Roth, Pierce A., Liem, Alvin T., Hill, Jessica M., Willis, Kristen L., Rossmaier, Rebecca D., Marinich, Andrew V., Maughan, Michele N., Karavis, Mark A., Kuhn, Jens H., Honko, Anna N., Rosenzweig, C. Nicole
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2016
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982148/
https://www.ncbi.nlm.nih.gov/pubmed/27191483
http://dx.doi.org/10.3201/eid2208.160270
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author Kilianski, Andy
Roth, Pierce A.
Liem, Alvin T.
Hill, Jessica M.
Willis, Kristen L.
Rossmaier, Rebecca D.
Marinich, Andrew V.
Maughan, Michele N.
Karavis, Mark A.
Kuhn, Jens H.
Honko, Anna N.
Rosenzweig, C. Nicole
author_facet Kilianski, Andy
Roth, Pierce A.
Liem, Alvin T.
Hill, Jessica M.
Willis, Kristen L.
Rossmaier, Rebecca D.
Marinich, Andrew V.
Maughan, Michele N.
Karavis, Mark A.
Kuhn, Jens H.
Honko, Anna N.
Rosenzweig, C. Nicole
author_sort Kilianski, Andy
collection PubMed
description Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis virus and Ebola virus in 3 hours from sample receipt to data acquisition, demonstrating a fieldable technique for RNA virus characterization.
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spelling pubmed-49821482016-08-12 Use of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses Kilianski, Andy Roth, Pierce A. Liem, Alvin T. Hill, Jessica M. Willis, Kristen L. Rossmaier, Rebecca D. Marinich, Andrew V. Maughan, Michele N. Karavis, Mark A. Kuhn, Jens H. Honko, Anna N. Rosenzweig, C. Nicole Emerg Infect Dis Dispatch Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis virus and Ebola virus in 3 hours from sample receipt to data acquisition, demonstrating a fieldable technique for RNA virus characterization. Centers for Disease Control and Prevention 2016-08 /pmc/articles/PMC4982148/ /pubmed/27191483 http://dx.doi.org/10.3201/eid2208.160270 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Kilianski, Andy
Roth, Pierce A.
Liem, Alvin T.
Hill, Jessica M.
Willis, Kristen L.
Rossmaier, Rebecca D.
Marinich, Andrew V.
Maughan, Michele N.
Karavis, Mark A.
Kuhn, Jens H.
Honko, Anna N.
Rosenzweig, C. Nicole
Use of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses
title Use of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses
title_full Use of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses
title_fullStr Use of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses
title_full_unstemmed Use of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses
title_short Use of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses
title_sort use of unamplified rna/cdna–hybrid nanopore sequencing for rapid detection and characterization of rna viruses
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982148/
https://www.ncbi.nlm.nih.gov/pubmed/27191483
http://dx.doi.org/10.3201/eid2208.160270
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