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The influence of trematode parasite burden on gene expression in a mammalian host
BACKGROUND: Parasites can profoundly impact their hosts and are responsible for a plethora of debilitating diseases. To identify global changes in host gene expression related to parasite infection, we sequenced, assembled, and annotated the liver transcriptomes of Balb/cj mice infected with the tre...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982272/ https://www.ncbi.nlm.nih.gov/pubmed/27514777 http://dx.doi.org/10.1186/s12864-016-2950-5 |
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author | Wijayawardena, Bhagya K. Minchella, Dennis J. DeWoody, J. Andrew |
author_facet | Wijayawardena, Bhagya K. Minchella, Dennis J. DeWoody, J. Andrew |
author_sort | Wijayawardena, Bhagya K. |
collection | PubMed |
description | BACKGROUND: Parasites can profoundly impact their hosts and are responsible for a plethora of debilitating diseases. To identify global changes in host gene expression related to parasite infection, we sequenced, assembled, and annotated the liver transcriptomes of Balb/cj mice infected with the trematode parasite Schistosoma mansoni and compared the results to uninfected mice. We used two different methodologies (i.e. de novo and reference guided) to evaluate the influence of parasite sequences on host transcriptome assembly. RESULTS: Our results demonstrate that the choice of assembly methodology significantly impacted the proportion of parasitic reads detected from the host library, yet the presence of non-target (xenobiotic) sequences did not create significant structural errors in the assembly. After removing parasite sequences from the mouse transcriptomes, we analyzed host gene expression under different parasite infection levels and observed significant differences in the associated immunologic and metabolic responses based on infection level. In particular, genes associated with T–helper type 1 (Th–1) and T–helper type 2 (Th–2) were up-regulated in infected mice whereas genes related to amino acid and carbohydrate metabolism were down-regulated in infected mice. These changes in gene expression scale with infection status and likely impact the evolutionary fitness of hosts. CONCLUSIONS: Overall, our data indicate that a) infected mice reduce the expression of key metabolic genes in direct proportion to their infection level; b) infected mice similarly increase the expression of key immune genes in response to infection; c) patterns of gene expression correspond to the pathological symptoms of schistosomiasis; and d) identifying and filtering out non-target sequences (xenobiotics) improves differential expression prediction. Our findings identify parasite targets for RNAi or other therapies and provide a better understanding of the pathology and host immune repertoire involved in response to S. mansoni infections. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-2950-5) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4982272 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-49822722016-08-13 The influence of trematode parasite burden on gene expression in a mammalian host Wijayawardena, Bhagya K. Minchella, Dennis J. DeWoody, J. Andrew BMC Genomics Research Article BACKGROUND: Parasites can profoundly impact their hosts and are responsible for a plethora of debilitating diseases. To identify global changes in host gene expression related to parasite infection, we sequenced, assembled, and annotated the liver transcriptomes of Balb/cj mice infected with the trematode parasite Schistosoma mansoni and compared the results to uninfected mice. We used two different methodologies (i.e. de novo and reference guided) to evaluate the influence of parasite sequences on host transcriptome assembly. RESULTS: Our results demonstrate that the choice of assembly methodology significantly impacted the proportion of parasitic reads detected from the host library, yet the presence of non-target (xenobiotic) sequences did not create significant structural errors in the assembly. After removing parasite sequences from the mouse transcriptomes, we analyzed host gene expression under different parasite infection levels and observed significant differences in the associated immunologic and metabolic responses based on infection level. In particular, genes associated with T–helper type 1 (Th–1) and T–helper type 2 (Th–2) were up-regulated in infected mice whereas genes related to amino acid and carbohydrate metabolism were down-regulated in infected mice. These changes in gene expression scale with infection status and likely impact the evolutionary fitness of hosts. CONCLUSIONS: Overall, our data indicate that a) infected mice reduce the expression of key metabolic genes in direct proportion to their infection level; b) infected mice similarly increase the expression of key immune genes in response to infection; c) patterns of gene expression correspond to the pathological symptoms of schistosomiasis; and d) identifying and filtering out non-target sequences (xenobiotics) improves differential expression prediction. Our findings identify parasite targets for RNAi or other therapies and provide a better understanding of the pathology and host immune repertoire involved in response to S. mansoni infections. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-2950-5) contains supplementary material, which is available to authorized users. BioMed Central 2016-08-11 /pmc/articles/PMC4982272/ /pubmed/27514777 http://dx.doi.org/10.1186/s12864-016-2950-5 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Wijayawardena, Bhagya K. Minchella, Dennis J. DeWoody, J. Andrew The influence of trematode parasite burden on gene expression in a mammalian host |
title | The influence of trematode parasite burden on gene expression in a mammalian host |
title_full | The influence of trematode parasite burden on gene expression in a mammalian host |
title_fullStr | The influence of trematode parasite burden on gene expression in a mammalian host |
title_full_unstemmed | The influence of trematode parasite burden on gene expression in a mammalian host |
title_short | The influence of trematode parasite burden on gene expression in a mammalian host |
title_sort | influence of trematode parasite burden on gene expression in a mammalian host |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982272/ https://www.ncbi.nlm.nih.gov/pubmed/27514777 http://dx.doi.org/10.1186/s12864-016-2950-5 |
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