Complete Genome Sequence of Escherichia coli ER1821R, a Laboratory K-12 Derivative Engineered To Be Deficient in All Methylcytosine and Methyladenine Restriction Systems

We present here the complete genomic sequence of a rifampin-resistant derivative of the Escherichia coli K-12 laboratory strain ER1821, engineered to be deficient in all known restriction systems, making it suitable for generating unbiased libraries from organisms with non-K-12 methylation patterns....

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Autores principales: Jobling, Michael G., Raleigh, Elisabeth A., Frank, Daniel N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Prokaryotes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982283/
https://www.ncbi.nlm.nih.gov/pubmed/27516504
http://dx.doi.org/10.1128/genomeA.00763-16
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author Jobling, Michael G.
Raleigh, Elisabeth A.
Frank, Daniel N.
author_facet Jobling, Michael G.
Raleigh, Elisabeth A.
Frank, Daniel N.
author_sort Jobling, Michael G.
collection PubMed
description We present here the complete genomic sequence of a rifampin-resistant derivative of the Escherichia coli K-12 laboratory strain ER1821, engineered to be deficient in all known restriction systems, making it suitable for generating unbiased libraries from organisms with non-K-12 methylation patterns. The ER1821R genome is most closely related to that of DH1, another popular cloning strain (both derived from MM294), but is deleted for the e14 prophage (McrA(-)) and the immigration control (McrBC(-) EcoKI R(-) M(-) Mrr(-)) loci.
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spelling pubmed-49822832016-08-17 Complete Genome Sequence of Escherichia coli ER1821R, a Laboratory K-12 Derivative Engineered To Be Deficient in All Methylcytosine and Methyladenine Restriction Systems Jobling, Michael G. Raleigh, Elisabeth A. Frank, Daniel N. Genome Announc Prokaryotes We present here the complete genomic sequence of a rifampin-resistant derivative of the Escherichia coli K-12 laboratory strain ER1821, engineered to be deficient in all known restriction systems, making it suitable for generating unbiased libraries from organisms with non-K-12 methylation patterns. The ER1821R genome is most closely related to that of DH1, another popular cloning strain (both derived from MM294), but is deleted for the e14 prophage (McrA(-)) and the immigration control (McrBC(-) EcoKI R(-) M(-) Mrr(-)) loci. American Society for Microbiology 2016-08-11 /pmc/articles/PMC4982283/ /pubmed/27516504 http://dx.doi.org/10.1128/genomeA.00763-16 Text en Copyright © 2016 Jobling et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Prokaryotes
Jobling, Michael G.
Raleigh, Elisabeth A.
Frank, Daniel N.
Complete Genome Sequence of Escherichia coli ER1821R, a Laboratory K-12 Derivative Engineered To Be Deficient in All Methylcytosine and Methyladenine Restriction Systems
title Complete Genome Sequence of Escherichia coli ER1821R, a Laboratory K-12 Derivative Engineered To Be Deficient in All Methylcytosine and Methyladenine Restriction Systems
title_full Complete Genome Sequence of Escherichia coli ER1821R, a Laboratory K-12 Derivative Engineered To Be Deficient in All Methylcytosine and Methyladenine Restriction Systems
title_fullStr Complete Genome Sequence of Escherichia coli ER1821R, a Laboratory K-12 Derivative Engineered To Be Deficient in All Methylcytosine and Methyladenine Restriction Systems
title_full_unstemmed Complete Genome Sequence of Escherichia coli ER1821R, a Laboratory K-12 Derivative Engineered To Be Deficient in All Methylcytosine and Methyladenine Restriction Systems
title_short Complete Genome Sequence of Escherichia coli ER1821R, a Laboratory K-12 Derivative Engineered To Be Deficient in All Methylcytosine and Methyladenine Restriction Systems
title_sort complete genome sequence of escherichia coli er1821r, a laboratory k-12 derivative engineered to be deficient in all methylcytosine and methyladenine restriction systems
topic Prokaryotes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982283/
https://www.ncbi.nlm.nih.gov/pubmed/27516504
http://dx.doi.org/10.1128/genomeA.00763-16
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