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Antagonistic Functions of USAG-1 and RUNX2 during Tooth Development
Supernumerary teeth and tooth agenesis are common morphological anomalies in humans. We previously obtained evidence that supernumerary maxillary incisors form as a result of the successive development of the rudimentary maxillary incisor tooth germ in Usag-1 null mice. The development of tooth germ...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982599/ https://www.ncbi.nlm.nih.gov/pubmed/27518316 http://dx.doi.org/10.1371/journal.pone.0161067 |
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author | Togo, Yumiko Takahashi, Katsu Saito, Kazuyuki Kiso, Honoka Tsukamoto, Hiroko Huang, Boyen Yanagita, Motoko Sugai, Manabu Harada, Hidemitsu Komori, Toshihisa Shimizu, Akira MacDougall, Mary Bessho, Kazuhisa |
author_facet | Togo, Yumiko Takahashi, Katsu Saito, Kazuyuki Kiso, Honoka Tsukamoto, Hiroko Huang, Boyen Yanagita, Motoko Sugai, Manabu Harada, Hidemitsu Komori, Toshihisa Shimizu, Akira MacDougall, Mary Bessho, Kazuhisa |
author_sort | Togo, Yumiko |
collection | PubMed |
description | Supernumerary teeth and tooth agenesis are common morphological anomalies in humans. We previously obtained evidence that supernumerary maxillary incisors form as a result of the successive development of the rudimentary maxillary incisor tooth germ in Usag-1 null mice. The development of tooth germs is arrested in Runx2 null mice, and such mice also exhibit lingual epithelial buds associated with the upper molars and incisors. The aim of this study is to investigate the potential crosstalk between Usag-1 and Runx2 during tooth development. In the present study, three interesting phenomena were observed in double null Usag-1(-/-)/Runx2(-/-) mice: the prevalence of supernumerary teeth was lower than in Usag-1 null mice; tooth development progressed further compared than in Runx2 null mice; and the frequency of molar lingual buds was lower than in Runx2 null mice. Therefore, we suggest that RUNX2 and USAG-1 act in an antagonistic manner. The lingual bud was completely filled with odontogenic epithelial Sox2-positive cells in the Usag-1(+/+)/Runx2(-/-) mice, whereas almost no odontogenic epithelial Sox2-positive cells contributed to supernumerary tooth formation in the rudimentary maxillary incisors of the Usag-1(-/-)/Runx2(+/+) mice. Our findings suggest that RUNX2 directly or indirectly prevents the differentiation and/or proliferation of odontogenic epithelial Sox2-positive cells. We hypothesize that RUNX2 inhibits the bone morphogenetic protein (BMP) and/or Wnt signaling pathways regulated by USAG-1, whereas RUNX2 expression is induced by BMP signaling independently of USAG-1. |
format | Online Article Text |
id | pubmed-4982599 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-49825992016-08-29 Antagonistic Functions of USAG-1 and RUNX2 during Tooth Development Togo, Yumiko Takahashi, Katsu Saito, Kazuyuki Kiso, Honoka Tsukamoto, Hiroko Huang, Boyen Yanagita, Motoko Sugai, Manabu Harada, Hidemitsu Komori, Toshihisa Shimizu, Akira MacDougall, Mary Bessho, Kazuhisa PLoS One Research Article Supernumerary teeth and tooth agenesis are common morphological anomalies in humans. We previously obtained evidence that supernumerary maxillary incisors form as a result of the successive development of the rudimentary maxillary incisor tooth germ in Usag-1 null mice. The development of tooth germs is arrested in Runx2 null mice, and such mice also exhibit lingual epithelial buds associated with the upper molars and incisors. The aim of this study is to investigate the potential crosstalk between Usag-1 and Runx2 during tooth development. In the present study, three interesting phenomena were observed in double null Usag-1(-/-)/Runx2(-/-) mice: the prevalence of supernumerary teeth was lower than in Usag-1 null mice; tooth development progressed further compared than in Runx2 null mice; and the frequency of molar lingual buds was lower than in Runx2 null mice. Therefore, we suggest that RUNX2 and USAG-1 act in an antagonistic manner. The lingual bud was completely filled with odontogenic epithelial Sox2-positive cells in the Usag-1(+/+)/Runx2(-/-) mice, whereas almost no odontogenic epithelial Sox2-positive cells contributed to supernumerary tooth formation in the rudimentary maxillary incisors of the Usag-1(-/-)/Runx2(+/+) mice. Our findings suggest that RUNX2 directly or indirectly prevents the differentiation and/or proliferation of odontogenic epithelial Sox2-positive cells. We hypothesize that RUNX2 inhibits the bone morphogenetic protein (BMP) and/or Wnt signaling pathways regulated by USAG-1, whereas RUNX2 expression is induced by BMP signaling independently of USAG-1. Public Library of Science 2016-08-12 /pmc/articles/PMC4982599/ /pubmed/27518316 http://dx.doi.org/10.1371/journal.pone.0161067 Text en © 2016 Togo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Togo, Yumiko Takahashi, Katsu Saito, Kazuyuki Kiso, Honoka Tsukamoto, Hiroko Huang, Boyen Yanagita, Motoko Sugai, Manabu Harada, Hidemitsu Komori, Toshihisa Shimizu, Akira MacDougall, Mary Bessho, Kazuhisa Antagonistic Functions of USAG-1 and RUNX2 during Tooth Development |
title | Antagonistic Functions of USAG-1 and RUNX2 during Tooth Development |
title_full | Antagonistic Functions of USAG-1 and RUNX2 during Tooth Development |
title_fullStr | Antagonistic Functions of USAG-1 and RUNX2 during Tooth Development |
title_full_unstemmed | Antagonistic Functions of USAG-1 and RUNX2 during Tooth Development |
title_short | Antagonistic Functions of USAG-1 and RUNX2 during Tooth Development |
title_sort | antagonistic functions of usag-1 and runx2 during tooth development |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982599/ https://www.ncbi.nlm.nih.gov/pubmed/27518316 http://dx.doi.org/10.1371/journal.pone.0161067 |
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