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Argonaute Family Protein Expression in Normal Tissue and Cancer Entities

The members of the Argonaute (AGO) protein family are key players in miRNA-guided gene silencing. They enable the interaction between small RNAs and their respective target mRNA(s) and support the catalytic destruction of the gene transcript or recruit additional proteins for downstream gene silenci...

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Autores principales: Völler, Daniel, Linck, Lisa, Bruckmann, Astrid, Hauptmann, Judith, Deutzmann, Rainer, Meister, Gunter, Bosserhoff, Anja Katrin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982624/
https://www.ncbi.nlm.nih.gov/pubmed/27518285
http://dx.doi.org/10.1371/journal.pone.0161165
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author Völler, Daniel
Linck, Lisa
Bruckmann, Astrid
Hauptmann, Judith
Deutzmann, Rainer
Meister, Gunter
Bosserhoff, Anja Katrin
author_facet Völler, Daniel
Linck, Lisa
Bruckmann, Astrid
Hauptmann, Judith
Deutzmann, Rainer
Meister, Gunter
Bosserhoff, Anja Katrin
author_sort Völler, Daniel
collection PubMed
description The members of the Argonaute (AGO) protein family are key players in miRNA-guided gene silencing. They enable the interaction between small RNAs and their respective target mRNA(s) and support the catalytic destruction of the gene transcript or recruit additional proteins for downstream gene silencing. The human AGO family consists of four AGO proteins (AGO1-AGO4), but only AGO2 harbors nuclease activity. In this study, we characterized the expression of the four AGO proteins in cancer cell lines and normal tissues with a new mass spectrometry approach called AGO-APP (AGO Affinity Purification by Peptides). In all analyzed normal tissues, AGO1 and AGO2 were most prominent, but marked tissue-specific differences were identified. Furthermore, considerable changes during development were observed by comparing fetal and adult tissues. We also identified decreased overall AGO expression in melanoma derived cell lines compared to other tumor cell lines and normal tissues, with the largest differences in AGO2 expression. The experiments described in this study suggest that reduced amounts of AGO proteins, as key players in miRNA processing, have impact on several cellular processes. Deregulated miRNA expression has been attributed to chromosomal aberrations, promoter regulation and it is known to have a major impact on tumor development and progression. Our findings will further increase our basic understanding of the molecular basis of miRNA processing and its relevance for disease.
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spelling pubmed-49826242016-08-29 Argonaute Family Protein Expression in Normal Tissue and Cancer Entities Völler, Daniel Linck, Lisa Bruckmann, Astrid Hauptmann, Judith Deutzmann, Rainer Meister, Gunter Bosserhoff, Anja Katrin PLoS One Research Article The members of the Argonaute (AGO) protein family are key players in miRNA-guided gene silencing. They enable the interaction between small RNAs and their respective target mRNA(s) and support the catalytic destruction of the gene transcript or recruit additional proteins for downstream gene silencing. The human AGO family consists of four AGO proteins (AGO1-AGO4), but only AGO2 harbors nuclease activity. In this study, we characterized the expression of the four AGO proteins in cancer cell lines and normal tissues with a new mass spectrometry approach called AGO-APP (AGO Affinity Purification by Peptides). In all analyzed normal tissues, AGO1 and AGO2 were most prominent, but marked tissue-specific differences were identified. Furthermore, considerable changes during development were observed by comparing fetal and adult tissues. We also identified decreased overall AGO expression in melanoma derived cell lines compared to other tumor cell lines and normal tissues, with the largest differences in AGO2 expression. The experiments described in this study suggest that reduced amounts of AGO proteins, as key players in miRNA processing, have impact on several cellular processes. Deregulated miRNA expression has been attributed to chromosomal aberrations, promoter regulation and it is known to have a major impact on tumor development and progression. Our findings will further increase our basic understanding of the molecular basis of miRNA processing and its relevance for disease. Public Library of Science 2016-08-12 /pmc/articles/PMC4982624/ /pubmed/27518285 http://dx.doi.org/10.1371/journal.pone.0161165 Text en © 2016 Völler et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Völler, Daniel
Linck, Lisa
Bruckmann, Astrid
Hauptmann, Judith
Deutzmann, Rainer
Meister, Gunter
Bosserhoff, Anja Katrin
Argonaute Family Protein Expression in Normal Tissue and Cancer Entities
title Argonaute Family Protein Expression in Normal Tissue and Cancer Entities
title_full Argonaute Family Protein Expression in Normal Tissue and Cancer Entities
title_fullStr Argonaute Family Protein Expression in Normal Tissue and Cancer Entities
title_full_unstemmed Argonaute Family Protein Expression in Normal Tissue and Cancer Entities
title_short Argonaute Family Protein Expression in Normal Tissue and Cancer Entities
title_sort argonaute family protein expression in normal tissue and cancer entities
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982624/
https://www.ncbi.nlm.nih.gov/pubmed/27518285
http://dx.doi.org/10.1371/journal.pone.0161165
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