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Highly Multiplexed Imaging Uncovers Changes in Compositional Noise within Assembling Focal Adhesions
Integrin adhesome proteins bind each other in alternative manners, forming within the cell diverse cell-matrix adhesion sites with distinct properties. An intriguing question is how such modular assembly of adhesion sites is achieved correctly solely by self-organization of their components. Here we...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982658/ https://www.ncbi.nlm.nih.gov/pubmed/27519053 http://dx.doi.org/10.1371/journal.pone.0160591 |
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author | Harizanova, Jana Fermin, Yessica Malik-Sheriff, Rahuman S. Wieczorek, Jakob Ickstadt, Katja Grecco, Hernán E. Zamir, Eli |
author_facet | Harizanova, Jana Fermin, Yessica Malik-Sheriff, Rahuman S. Wieczorek, Jakob Ickstadt, Katja Grecco, Hernán E. Zamir, Eli |
author_sort | Harizanova, Jana |
collection | PubMed |
description | Integrin adhesome proteins bind each other in alternative manners, forming within the cell diverse cell-matrix adhesion sites with distinct properties. An intriguing question is how such modular assembly of adhesion sites is achieved correctly solely by self-organization of their components. Here we address this question using high-throughput multiplexed imaging of eight proteins and two phosphorylation sites in a large number of single focal adhesions. We found that during the assembly of focal adhesions the variances of protein densities decrease while the correlations between them increase, suggesting reduction in the noise levels within these structures. These changes correlate independently with the area and internal density of focal adhesions, but not with their age or shape. Artificial neural network analysis indicates that a joint consideration of multiple components improves the predictability of paxillin and zyxin levels in internally dense focal adhesions. This suggests that paxillin and zyxin densities in focal adhesions are fine-tuned by integrating the levels of multiple other components, thus averaging-out stochastic fluctuations. Based on these results we propose that increase in internal protein densities facilitates noise suppression in focal adhesions, while noise suppression enables their stable growth and further density increase—hence forming a feedback loop giving rise to a quality-controlled assembly. |
format | Online Article Text |
id | pubmed-4982658 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-49826582016-08-29 Highly Multiplexed Imaging Uncovers Changes in Compositional Noise within Assembling Focal Adhesions Harizanova, Jana Fermin, Yessica Malik-Sheriff, Rahuman S. Wieczorek, Jakob Ickstadt, Katja Grecco, Hernán E. Zamir, Eli PLoS One Research Article Integrin adhesome proteins bind each other in alternative manners, forming within the cell diverse cell-matrix adhesion sites with distinct properties. An intriguing question is how such modular assembly of adhesion sites is achieved correctly solely by self-organization of their components. Here we address this question using high-throughput multiplexed imaging of eight proteins and two phosphorylation sites in a large number of single focal adhesions. We found that during the assembly of focal adhesions the variances of protein densities decrease while the correlations between them increase, suggesting reduction in the noise levels within these structures. These changes correlate independently with the area and internal density of focal adhesions, but not with their age or shape. Artificial neural network analysis indicates that a joint consideration of multiple components improves the predictability of paxillin and zyxin levels in internally dense focal adhesions. This suggests that paxillin and zyxin densities in focal adhesions are fine-tuned by integrating the levels of multiple other components, thus averaging-out stochastic fluctuations. Based on these results we propose that increase in internal protein densities facilitates noise suppression in focal adhesions, while noise suppression enables their stable growth and further density increase—hence forming a feedback loop giving rise to a quality-controlled assembly. Public Library of Science 2016-08-12 /pmc/articles/PMC4982658/ /pubmed/27519053 http://dx.doi.org/10.1371/journal.pone.0160591 Text en © 2016 Harizanova et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Harizanova, Jana Fermin, Yessica Malik-Sheriff, Rahuman S. Wieczorek, Jakob Ickstadt, Katja Grecco, Hernán E. Zamir, Eli Highly Multiplexed Imaging Uncovers Changes in Compositional Noise within Assembling Focal Adhesions |
title | Highly Multiplexed Imaging Uncovers Changes in Compositional Noise within Assembling Focal Adhesions |
title_full | Highly Multiplexed Imaging Uncovers Changes in Compositional Noise within Assembling Focal Adhesions |
title_fullStr | Highly Multiplexed Imaging Uncovers Changes in Compositional Noise within Assembling Focal Adhesions |
title_full_unstemmed | Highly Multiplexed Imaging Uncovers Changes in Compositional Noise within Assembling Focal Adhesions |
title_short | Highly Multiplexed Imaging Uncovers Changes in Compositional Noise within Assembling Focal Adhesions |
title_sort | highly multiplexed imaging uncovers changes in compositional noise within assembling focal adhesions |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4982658/ https://www.ncbi.nlm.nih.gov/pubmed/27519053 http://dx.doi.org/10.1371/journal.pone.0160591 |
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