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Possible healthcare-associated transmission as a cause of secondary infection and population structure of Staphylococcus aureus isolates from two wound treatment centres in Ghana

We have previously shown that secondary infections of Buruli ulcer wounds were frequently caused by Staphylococcus aureus. To gain understanding into possible routes of secondary infection, we characterized S. aureus isolates from patient lesions and surrounding environments across two Ghanaian heal...

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Autores principales: Kpeli, G., Darko Otchere, I., Lamelas, A., Buultjens, A.L., Bulach, D., Baines, S.L., Seemann, T., Giulieri, S., Nakobu, Z., Aboagye, S.Y., Owusu-Mireku, E., Pluschke, G., Stinear, T.P., Yeboah-Manu, D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4983152/
https://www.ncbi.nlm.nih.gov/pubmed/27547406
http://dx.doi.org/10.1016/j.nmni.2016.07.001
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author Kpeli, G.
Darko Otchere, I.
Lamelas, A.
Buultjens, A.L.
Bulach, D.
Baines, S.L.
Seemann, T.
Giulieri, S.
Nakobu, Z.
Aboagye, S.Y.
Owusu-Mireku, E.
Pluschke, G.
Stinear, T.P.
Yeboah-Manu, D.
author_facet Kpeli, G.
Darko Otchere, I.
Lamelas, A.
Buultjens, A.L.
Bulach, D.
Baines, S.L.
Seemann, T.
Giulieri, S.
Nakobu, Z.
Aboagye, S.Y.
Owusu-Mireku, E.
Pluschke, G.
Stinear, T.P.
Yeboah-Manu, D.
author_sort Kpeli, G.
collection PubMed
description We have previously shown that secondary infections of Buruli ulcer wounds were frequently caused by Staphylococcus aureus. To gain understanding into possible routes of secondary infection, we characterized S. aureus isolates from patient lesions and surrounding environments across two Ghanaian health centres. One hundred and one S. aureus isolates were isolated from wounds (n = 93, 92.1%) and the hospital environment (n = 8, 7.9%) and characterized by the spa gene, mecA and the Panton–Valentine leucocidin toxin followed by spa sequencing and whole genome sequencing of a subset of 49 isolates. Spa typing and sequencing of the spa gene from 91 isolates identified 29 different spa types with t355 (ST152), t186 (ST88), and t346 dominating. Although many distinct strains were isolated from both health centres, genotype clustering was identified within centres. In addition, we identified a cluster consisting of isolates from a healthcare worker, patients dressed that same day and forceps used for dressing, pointing to possible healthcare-associated transmission. These clusters were confirmed by phylogenomic analysis. Twenty-four (22.8%) isolates were identified as methicillin-resistant S. aureus and lukFS genes encoding Panton–Valentine leucocidin were identified in 67 (63.8%) of the isolates. Phenotype screening showed widespread resistance to tetracycline, erythromycin, rifampicin, amikacin and streptomycin. Genomics confirmed the widespread presence of antibiotic resistance genes to β-lactams, chloramphenicol, trimethoprim, quinolone, streptomycin and tetracycline. Our findings indicate that the healthcare environment probably contributes to the superinfection of Buruli ulcer wounds and calls for improved training in wound management and infection control techniques.
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spelling pubmed-49831522016-08-19 Possible healthcare-associated transmission as a cause of secondary infection and population structure of Staphylococcus aureus isolates from two wound treatment centres in Ghana Kpeli, G. Darko Otchere, I. Lamelas, A. Buultjens, A.L. Bulach, D. Baines, S.L. Seemann, T. Giulieri, S. Nakobu, Z. Aboagye, S.Y. Owusu-Mireku, E. Pluschke, G. Stinear, T.P. Yeboah-Manu, D. New Microbes New Infect Original Article We have previously shown that secondary infections of Buruli ulcer wounds were frequently caused by Staphylococcus aureus. To gain understanding into possible routes of secondary infection, we characterized S. aureus isolates from patient lesions and surrounding environments across two Ghanaian health centres. One hundred and one S. aureus isolates were isolated from wounds (n = 93, 92.1%) and the hospital environment (n = 8, 7.9%) and characterized by the spa gene, mecA and the Panton–Valentine leucocidin toxin followed by spa sequencing and whole genome sequencing of a subset of 49 isolates. Spa typing and sequencing of the spa gene from 91 isolates identified 29 different spa types with t355 (ST152), t186 (ST88), and t346 dominating. Although many distinct strains were isolated from both health centres, genotype clustering was identified within centres. In addition, we identified a cluster consisting of isolates from a healthcare worker, patients dressed that same day and forceps used for dressing, pointing to possible healthcare-associated transmission. These clusters were confirmed by phylogenomic analysis. Twenty-four (22.8%) isolates were identified as methicillin-resistant S. aureus and lukFS genes encoding Panton–Valentine leucocidin were identified in 67 (63.8%) of the isolates. Phenotype screening showed widespread resistance to tetracycline, erythromycin, rifampicin, amikacin and streptomycin. Genomics confirmed the widespread presence of antibiotic resistance genes to β-lactams, chloramphenicol, trimethoprim, quinolone, streptomycin and tetracycline. Our findings indicate that the healthcare environment probably contributes to the superinfection of Buruli ulcer wounds and calls for improved training in wound management and infection control techniques. Elsevier 2016-07-12 /pmc/articles/PMC4983152/ /pubmed/27547406 http://dx.doi.org/10.1016/j.nmni.2016.07.001 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Article
Kpeli, G.
Darko Otchere, I.
Lamelas, A.
Buultjens, A.L.
Bulach, D.
Baines, S.L.
Seemann, T.
Giulieri, S.
Nakobu, Z.
Aboagye, S.Y.
Owusu-Mireku, E.
Pluschke, G.
Stinear, T.P.
Yeboah-Manu, D.
Possible healthcare-associated transmission as a cause of secondary infection and population structure of Staphylococcus aureus isolates from two wound treatment centres in Ghana
title Possible healthcare-associated transmission as a cause of secondary infection and population structure of Staphylococcus aureus isolates from two wound treatment centres in Ghana
title_full Possible healthcare-associated transmission as a cause of secondary infection and population structure of Staphylococcus aureus isolates from two wound treatment centres in Ghana
title_fullStr Possible healthcare-associated transmission as a cause of secondary infection and population structure of Staphylococcus aureus isolates from two wound treatment centres in Ghana
title_full_unstemmed Possible healthcare-associated transmission as a cause of secondary infection and population structure of Staphylococcus aureus isolates from two wound treatment centres in Ghana
title_short Possible healthcare-associated transmission as a cause of secondary infection and population structure of Staphylococcus aureus isolates from two wound treatment centres in Ghana
title_sort possible healthcare-associated transmission as a cause of secondary infection and population structure of staphylococcus aureus isolates from two wound treatment centres in ghana
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4983152/
https://www.ncbi.nlm.nih.gov/pubmed/27547406
http://dx.doi.org/10.1016/j.nmni.2016.07.001
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