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Beneficial Effect of Short Pretransplant Period of Hypothermic Pulsatile Perfusion of the Warm-Ischemic Kidney after Cold Storage: Experimental Study

Warm ischemia (WI) produces a significant deleterious effect in potential kidney grafts. Hypothermic machine perfusion (HMP) seems to improve immediate graft function after transplant. Our aim was to analyze the effect of short pretransplant periods of pulsatile HMP on histology and renal injury in...

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Autores principales: Lázaro, Alberto, Humanes, Blanca, Jado, Juan Carlos, Mojena, Marina, González-Nicolás, María Ángeles, del Cañizo, Juan Francisco, Tejedor, Alberto, Lledó-García, Enrique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4983324/
https://www.ncbi.nlm.nih.gov/pubmed/27556029
http://dx.doi.org/10.1155/2016/2518626
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author Lázaro, Alberto
Humanes, Blanca
Jado, Juan Carlos
Mojena, Marina
González-Nicolás, María Ángeles
del Cañizo, Juan Francisco
Tejedor, Alberto
Lledó-García, Enrique
author_facet Lázaro, Alberto
Humanes, Blanca
Jado, Juan Carlos
Mojena, Marina
González-Nicolás, María Ángeles
del Cañizo, Juan Francisco
Tejedor, Alberto
Lledó-García, Enrique
author_sort Lázaro, Alberto
collection PubMed
description Warm ischemia (WI) produces a significant deleterious effect in potential kidney grafts. Hypothermic machine perfusion (HMP) seems to improve immediate graft function after transplant. Our aim was to analyze the effect of short pretransplant periods of pulsatile HMP on histology and renal injury in warm-ischemic kidneys. Twelve minipigs were used. WI was achieved in the right kidney by applying a vascular clamp for 45 min. After nephrectomy, autotransplant was performed following one of two strategies: cold storage of the kidneys or cold storage combined with perfusion in pulsatile HMP. The graft was removed early to study renal morphology, inflammation (fibrosis), and apoptosis. Proinflammatory activity and fibrosis were less pronounced after cold storage of the kidneys with HMP than after cold storage only. The use of HMP also decreased apoptosis compared with cold storage only. The detrimental effects on cells of an initial and prolonged period of WI seem to improve with a preservation protocol that includes a short period of pulsatile HMP after cold storage and immediately before the transplant, in comparison with cold storage only.
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spelling pubmed-49833242016-08-23 Beneficial Effect of Short Pretransplant Period of Hypothermic Pulsatile Perfusion of the Warm-Ischemic Kidney after Cold Storage: Experimental Study Lázaro, Alberto Humanes, Blanca Jado, Juan Carlos Mojena, Marina González-Nicolás, María Ángeles del Cañizo, Juan Francisco Tejedor, Alberto Lledó-García, Enrique Biomed Res Int Research Article Warm ischemia (WI) produces a significant deleterious effect in potential kidney grafts. Hypothermic machine perfusion (HMP) seems to improve immediate graft function after transplant. Our aim was to analyze the effect of short pretransplant periods of pulsatile HMP on histology and renal injury in warm-ischemic kidneys. Twelve minipigs were used. WI was achieved in the right kidney by applying a vascular clamp for 45 min. After nephrectomy, autotransplant was performed following one of two strategies: cold storage of the kidneys or cold storage combined with perfusion in pulsatile HMP. The graft was removed early to study renal morphology, inflammation (fibrosis), and apoptosis. Proinflammatory activity and fibrosis were less pronounced after cold storage of the kidneys with HMP than after cold storage only. The use of HMP also decreased apoptosis compared with cold storage only. The detrimental effects on cells of an initial and prolonged period of WI seem to improve with a preservation protocol that includes a short period of pulsatile HMP after cold storage and immediately before the transplant, in comparison with cold storage only. Hindawi Publishing Corporation 2016 2016-07-31 /pmc/articles/PMC4983324/ /pubmed/27556029 http://dx.doi.org/10.1155/2016/2518626 Text en Copyright © 2016 Alberto Lázaro et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lázaro, Alberto
Humanes, Blanca
Jado, Juan Carlos
Mojena, Marina
González-Nicolás, María Ángeles
del Cañizo, Juan Francisco
Tejedor, Alberto
Lledó-García, Enrique
Beneficial Effect of Short Pretransplant Period of Hypothermic Pulsatile Perfusion of the Warm-Ischemic Kidney after Cold Storage: Experimental Study
title Beneficial Effect of Short Pretransplant Period of Hypothermic Pulsatile Perfusion of the Warm-Ischemic Kidney after Cold Storage: Experimental Study
title_full Beneficial Effect of Short Pretransplant Period of Hypothermic Pulsatile Perfusion of the Warm-Ischemic Kidney after Cold Storage: Experimental Study
title_fullStr Beneficial Effect of Short Pretransplant Period of Hypothermic Pulsatile Perfusion of the Warm-Ischemic Kidney after Cold Storage: Experimental Study
title_full_unstemmed Beneficial Effect of Short Pretransplant Period of Hypothermic Pulsatile Perfusion of the Warm-Ischemic Kidney after Cold Storage: Experimental Study
title_short Beneficial Effect of Short Pretransplant Period of Hypothermic Pulsatile Perfusion of the Warm-Ischemic Kidney after Cold Storage: Experimental Study
title_sort beneficial effect of short pretransplant period of hypothermic pulsatile perfusion of the warm-ischemic kidney after cold storage: experimental study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4983324/
https://www.ncbi.nlm.nih.gov/pubmed/27556029
http://dx.doi.org/10.1155/2016/2518626
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