Cargando…

Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver

Rheumatoid arthritis (RA) is a chronic inflammatory disease, leading to progressive destruction of joints and extra-articular tissues, including organs such as liver and spleen. The purpose of this study was to compare the effects of a potential immunomodulator, natural polyphenol N-feruloylserotoni...

Descripción completa

Detalles Bibliográficos
Autores principales: Pašková, Ľudmila, Kuncírová, Viera, Poništ, Silvester, Mihálová, Danica, Nosáľ, Radomír, Harmatha, Juraj, Hrádková, Iveta, Čavojský, Tomáš, Bilka, František, Šišková, Katarína, Paulíková, Ingrid, Bezáková, Lýdia, Bauerová, Katarína
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4983360/
https://www.ncbi.nlm.nih.gov/pubmed/27556049
http://dx.doi.org/10.1155/2016/7509653
_version_ 1782447896361172992
author Pašková, Ľudmila
Kuncírová, Viera
Poništ, Silvester
Mihálová, Danica
Nosáľ, Radomír
Harmatha, Juraj
Hrádková, Iveta
Čavojský, Tomáš
Bilka, František
Šišková, Katarína
Paulíková, Ingrid
Bezáková, Lýdia
Bauerová, Katarína
author_facet Pašková, Ľudmila
Kuncírová, Viera
Poništ, Silvester
Mihálová, Danica
Nosáľ, Radomír
Harmatha, Juraj
Hrádková, Iveta
Čavojský, Tomáš
Bilka, František
Šišková, Katarína
Paulíková, Ingrid
Bezáková, Lýdia
Bauerová, Katarína
author_sort Pašková, Ľudmila
collection PubMed
description Rheumatoid arthritis (RA) is a chronic inflammatory disease, leading to progressive destruction of joints and extra-articular tissues, including organs such as liver and spleen. The purpose of this study was to compare the effects of a potential immunomodulator, natural polyphenol N-feruloylserotonin (N-f-5HT), with methotrexate (MTX), the standard in RA therapy, in the chronic phase of adjuvant-induced arthritis (AA) in male Lewis rats. The experiment included healthy controls (CO), arthritic animals (AA), AA given N-f-5HT (AA-N-f-5HT), and AA given MTX (AA-MTX). N-f-5HT did not affect the body weight change and clinical parameters until the 14th experimental day. Its positive effect was rising during the 28-day experiment, indicating a delayed onset of N-f-5HT action. Administration of either N-f-5HT or MTX caused reduction of inflammation measured as the level of CRP in plasma and the activity of LOX in the liver. mRNA transcription of TNF-α and iNOS in the liver was significantly attenuated in both MTX and N-f-5HT treated groups of arthritic rats. Interestingly, in contrast to MTX, N-f-5HT significantly lowered the level of IL-1β in plasma and IL-1β mRNA expression in the liver and spleen of arthritic rats. This speaks for future investigations of N-f-5HT as an agent in the treatment of RA in combination therapy with MTX.
format Online
Article
Text
id pubmed-4983360
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-49833602016-08-23 Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver Pašková, Ľudmila Kuncírová, Viera Poništ, Silvester Mihálová, Danica Nosáľ, Radomír Harmatha, Juraj Hrádková, Iveta Čavojský, Tomáš Bilka, František Šišková, Katarína Paulíková, Ingrid Bezáková, Lýdia Bauerová, Katarína J Immunol Res Research Article Rheumatoid arthritis (RA) is a chronic inflammatory disease, leading to progressive destruction of joints and extra-articular tissues, including organs such as liver and spleen. The purpose of this study was to compare the effects of a potential immunomodulator, natural polyphenol N-feruloylserotonin (N-f-5HT), with methotrexate (MTX), the standard in RA therapy, in the chronic phase of adjuvant-induced arthritis (AA) in male Lewis rats. The experiment included healthy controls (CO), arthritic animals (AA), AA given N-f-5HT (AA-N-f-5HT), and AA given MTX (AA-MTX). N-f-5HT did not affect the body weight change and clinical parameters until the 14th experimental day. Its positive effect was rising during the 28-day experiment, indicating a delayed onset of N-f-5HT action. Administration of either N-f-5HT or MTX caused reduction of inflammation measured as the level of CRP in plasma and the activity of LOX in the liver. mRNA transcription of TNF-α and iNOS in the liver was significantly attenuated in both MTX and N-f-5HT treated groups of arthritic rats. Interestingly, in contrast to MTX, N-f-5HT significantly lowered the level of IL-1β in plasma and IL-1β mRNA expression in the liver and spleen of arthritic rats. This speaks for future investigations of N-f-5HT as an agent in the treatment of RA in combination therapy with MTX. Hindawi Publishing Corporation 2016 2016-07-31 /pmc/articles/PMC4983360/ /pubmed/27556049 http://dx.doi.org/10.1155/2016/7509653 Text en Copyright © 2016 Ľudmila Pašková et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pašková, Ľudmila
Kuncírová, Viera
Poništ, Silvester
Mihálová, Danica
Nosáľ, Radomír
Harmatha, Juraj
Hrádková, Iveta
Čavojský, Tomáš
Bilka, František
Šišková, Katarína
Paulíková, Ingrid
Bezáková, Lýdia
Bauerová, Katarína
Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver
title Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver
title_full Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver
title_fullStr Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver
title_full_unstemmed Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver
title_short Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver
title_sort effect of n-feruloylserotonin and methotrexate on severity of experimental arthritis and on messenger rna expression of key proinflammatory markers in liver
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4983360/
https://www.ncbi.nlm.nih.gov/pubmed/27556049
http://dx.doi.org/10.1155/2016/7509653
work_keys_str_mv AT paskovaludmila effectofnferuloylserotoninandmethotrexateonseverityofexperimentalarthritisandonmessengerrnaexpressionofkeyproinflammatorymarkersinliver
AT kuncirovaviera effectofnferuloylserotoninandmethotrexateonseverityofexperimentalarthritisandonmessengerrnaexpressionofkeyproinflammatorymarkersinliver
AT ponistsilvester effectofnferuloylserotoninandmethotrexateonseverityofexperimentalarthritisandonmessengerrnaexpressionofkeyproinflammatorymarkersinliver
AT mihalovadanica effectofnferuloylserotoninandmethotrexateonseverityofexperimentalarthritisandonmessengerrnaexpressionofkeyproinflammatorymarkersinliver
AT nosalradomir effectofnferuloylserotoninandmethotrexateonseverityofexperimentalarthritisandonmessengerrnaexpressionofkeyproinflammatorymarkersinliver
AT harmathajuraj effectofnferuloylserotoninandmethotrexateonseverityofexperimentalarthritisandonmessengerrnaexpressionofkeyproinflammatorymarkersinliver
AT hradkovaiveta effectofnferuloylserotoninandmethotrexateonseverityofexperimentalarthritisandonmessengerrnaexpressionofkeyproinflammatorymarkersinliver
AT cavojskytomas effectofnferuloylserotoninandmethotrexateonseverityofexperimentalarthritisandonmessengerrnaexpressionofkeyproinflammatorymarkersinliver
AT bilkafrantisek effectofnferuloylserotoninandmethotrexateonseverityofexperimentalarthritisandonmessengerrnaexpressionofkeyproinflammatorymarkersinliver
AT siskovakatarina effectofnferuloylserotoninandmethotrexateonseverityofexperimentalarthritisandonmessengerrnaexpressionofkeyproinflammatorymarkersinliver
AT paulikovaingrid effectofnferuloylserotoninandmethotrexateonseverityofexperimentalarthritisandonmessengerrnaexpressionofkeyproinflammatorymarkersinliver
AT bezakovalydia effectofnferuloylserotoninandmethotrexateonseverityofexperimentalarthritisandonmessengerrnaexpressionofkeyproinflammatorymarkersinliver
AT bauerovakatarina effectofnferuloylserotoninandmethotrexateonseverityofexperimentalarthritisandonmessengerrnaexpressionofkeyproinflammatorymarkersinliver