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TLR2-Dependent Signaling for IL-15 Production Is Essential for the Homeostasis of Intestinal Intraepithelial Lymphocytes

TLR2 signaling is related to colitis and involved in regulation of innate immunity in the intestinal tract, but the mechanisms remain unclear. The aim of this study is to investigate how TLR2 affects differentiation of intraepithelial lymphocytes (IELs) and regulates the susceptibility of colitis. I...

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Autores principales: Qiu, Yuan, Pu, Aimin, Zheng, Hong, Liu, Minqiang, Chen, Weigang, Wang, Wensheng, Xiao, Weidong, Yang, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4983668/
https://www.ncbi.nlm.nih.gov/pubmed/27563173
http://dx.doi.org/10.1155/2016/4281865
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author Qiu, Yuan
Pu, Aimin
Zheng, Hong
Liu, Minqiang
Chen, Weigang
Wang, Wensheng
Xiao, Weidong
Yang, Hua
author_facet Qiu, Yuan
Pu, Aimin
Zheng, Hong
Liu, Minqiang
Chen, Weigang
Wang, Wensheng
Xiao, Weidong
Yang, Hua
author_sort Qiu, Yuan
collection PubMed
description TLR2 signaling is related to colitis and involved in regulation of innate immunity in the intestinal tract, but the mechanisms remain unclear. The aim of this study is to investigate how TLR2 affects differentiation of intraepithelial lymphocytes (IELs) and regulates the susceptibility of colitis. IELs were isolated from the small intestine and colon of mice, respectively. The IEL phenotype, activation, and apoptosis were examined using flow cytometry and RT-PCR. IL-15 expression and IEL location were detected through immunohistochemistry. The experimental colitis was induced by administration of dextran sulfate sodium (DSS). We found that the numbers of CD8αα (+), CD8αβ (+), and TCRγδ (+) IELs were significantly decreased in TLR2-deficient mice and the residual IELs displayed reduced activation and proliferation and increased apoptosis, accompanied with impaired IL-15 expression by intestinal epithelial cells (IECs). Further study showed that TLR2 signaling maintained the expression of IL-15 in IEC via NF-κB activation. Moreover, TLR2-deficient mice were found to be more susceptible to DSS-induced colitis as shown by the increased severity of colitis. Our results demonstrate that IECs contribute to the maintenance of IELs at least partly via TLR2-dependent IL-15 production, which provides a clue that may link IECs to innate immune protection of the host via IELs.
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spelling pubmed-49836682016-08-25 TLR2-Dependent Signaling for IL-15 Production Is Essential for the Homeostasis of Intestinal Intraepithelial Lymphocytes Qiu, Yuan Pu, Aimin Zheng, Hong Liu, Minqiang Chen, Weigang Wang, Wensheng Xiao, Weidong Yang, Hua Mediators Inflamm Research Article TLR2 signaling is related to colitis and involved in regulation of innate immunity in the intestinal tract, but the mechanisms remain unclear. The aim of this study is to investigate how TLR2 affects differentiation of intraepithelial lymphocytes (IELs) and regulates the susceptibility of colitis. IELs were isolated from the small intestine and colon of mice, respectively. The IEL phenotype, activation, and apoptosis were examined using flow cytometry and RT-PCR. IL-15 expression and IEL location were detected through immunohistochemistry. The experimental colitis was induced by administration of dextran sulfate sodium (DSS). We found that the numbers of CD8αα (+), CD8αβ (+), and TCRγδ (+) IELs were significantly decreased in TLR2-deficient mice and the residual IELs displayed reduced activation and proliferation and increased apoptosis, accompanied with impaired IL-15 expression by intestinal epithelial cells (IECs). Further study showed that TLR2 signaling maintained the expression of IL-15 in IEC via NF-κB activation. Moreover, TLR2-deficient mice were found to be more susceptible to DSS-induced colitis as shown by the increased severity of colitis. Our results demonstrate that IECs contribute to the maintenance of IELs at least partly via TLR2-dependent IL-15 production, which provides a clue that may link IECs to innate immune protection of the host via IELs. Hindawi Publishing Corporation 2016 2016-08-01 /pmc/articles/PMC4983668/ /pubmed/27563173 http://dx.doi.org/10.1155/2016/4281865 Text en Copyright © 2016 Yuan Qiu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Qiu, Yuan
Pu, Aimin
Zheng, Hong
Liu, Minqiang
Chen, Weigang
Wang, Wensheng
Xiao, Weidong
Yang, Hua
TLR2-Dependent Signaling for IL-15 Production Is Essential for the Homeostasis of Intestinal Intraepithelial Lymphocytes
title TLR2-Dependent Signaling for IL-15 Production Is Essential for the Homeostasis of Intestinal Intraepithelial Lymphocytes
title_full TLR2-Dependent Signaling for IL-15 Production Is Essential for the Homeostasis of Intestinal Intraepithelial Lymphocytes
title_fullStr TLR2-Dependent Signaling for IL-15 Production Is Essential for the Homeostasis of Intestinal Intraepithelial Lymphocytes
title_full_unstemmed TLR2-Dependent Signaling for IL-15 Production Is Essential for the Homeostasis of Intestinal Intraepithelial Lymphocytes
title_short TLR2-Dependent Signaling for IL-15 Production Is Essential for the Homeostasis of Intestinal Intraepithelial Lymphocytes
title_sort tlr2-dependent signaling for il-15 production is essential for the homeostasis of intestinal intraepithelial lymphocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4983668/
https://www.ncbi.nlm.nih.gov/pubmed/27563173
http://dx.doi.org/10.1155/2016/4281865
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