A Small Aptamer with Strong and Specific Recognition of the Triphosphate of ATP

[Image: see text] We report the in vitro selection of an RNA-based ATP aptamer with the ability to discriminate between adenosine ligands based on their 5‘ phosphorylation state. Previous selection of ATP aptamers yielded molecules that do not significantly discriminate between ligands at the 5‘ pos...

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Autores principales: Sazani, Peter L., Larralde, Rosa, Szostak, Jack W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2004
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4983724/
https://www.ncbi.nlm.nih.gov/pubmed/15237981
http://dx.doi.org/10.1021/ja049171k
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author Sazani, Peter L.
Larralde, Rosa
Szostak, Jack W.
author_facet Sazani, Peter L.
Larralde, Rosa
Szostak, Jack W.
author_sort Sazani, Peter L.
collection PubMed
description [Image: see text] We report the in vitro selection of an RNA-based ATP aptamer with the ability to discriminate between adenosine ligands based on their 5‘ phosphorylation state. Previous selection of ATP aptamers yielded molecules that do not significantly discriminate between ligands at the 5‘ position. By applying a selective pressure that demands recognition of the 5‘ triphosphate, we obtained an aptamer that binds to ATP with a K(d) of approximately 5 μM, and to AMP with a K(d) of approximately 5.5 mM, a difference of 1100-fold. This aptamer demonstrates the ability of small RNAs to interact with negatively charged moieties.
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spelling pubmed-49837242016-08-17 A Small Aptamer with Strong and Specific Recognition of the Triphosphate of ATP Sazani, Peter L. Larralde, Rosa Szostak, Jack W. J Am Chem Soc [Image: see text] We report the in vitro selection of an RNA-based ATP aptamer with the ability to discriminate between adenosine ligands based on their 5‘ phosphorylation state. Previous selection of ATP aptamers yielded molecules that do not significantly discriminate between ligands at the 5‘ position. By applying a selective pressure that demands recognition of the 5‘ triphosphate, we obtained an aptamer that binds to ATP with a K(d) of approximately 5 μM, and to AMP with a K(d) of approximately 5.5 mM, a difference of 1100-fold. This aptamer demonstrates the ability of small RNAs to interact with negatively charged moieties. American Chemical Society 2004-06-17 2004-07-14 /pmc/articles/PMC4983724/ /pubmed/15237981 http://dx.doi.org/10.1021/ja049171k Text en Copyright © 2004 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Sazani, Peter L.
Larralde, Rosa
Szostak, Jack W.
A Small Aptamer with Strong and Specific Recognition of the Triphosphate of ATP
title A Small Aptamer with Strong and Specific Recognition of the Triphosphate of ATP
title_full A Small Aptamer with Strong and Specific Recognition of the Triphosphate of ATP
title_fullStr A Small Aptamer with Strong and Specific Recognition of the Triphosphate of ATP
title_full_unstemmed A Small Aptamer with Strong and Specific Recognition of the Triphosphate of ATP
title_short A Small Aptamer with Strong and Specific Recognition of the Triphosphate of ATP
title_sort small aptamer with strong and specific recognition of the triphosphate of atp
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4983724/
https://www.ncbi.nlm.nih.gov/pubmed/15237981
http://dx.doi.org/10.1021/ja049171k
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