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Diversity of high risk human papilloma viruses in women treated with antiretroviral and in healthy controls and discordance with cervical dysplasia in the South of Benin

BACKGROUND: High risk oncologic Human Papilloma Virus (HPV) is one of the leading causes of cervical cancer worldwide. We investigated HPV genotypes among women living or not with Human Immuno-deficiency Virus (HIV) in two major hospitals in the south of the republic of BENIN in the city of Cotonou....

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Autores principales: Capo-chichi, Callinice D., Aguida, Blanche, Chabi, Nicodème W., Acapko-Ezin, Jocelyn, Sossah-Hiffo, Jonas, Agossou, Vidéhouénou K., Anagbla, Toussain, Zannou, Marcel, Houngbé, Fabien, Sanni, Ambaliou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4983786/
https://www.ncbi.nlm.nih.gov/pubmed/27528886
http://dx.doi.org/10.1186/s13027-016-0090-9
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author Capo-chichi, Callinice D.
Aguida, Blanche
Chabi, Nicodème W.
Acapko-Ezin, Jocelyn
Sossah-Hiffo, Jonas
Agossou, Vidéhouénou K.
Anagbla, Toussain
Zannou, Marcel
Houngbé, Fabien
Sanni, Ambaliou
author_facet Capo-chichi, Callinice D.
Aguida, Blanche
Chabi, Nicodème W.
Acapko-Ezin, Jocelyn
Sossah-Hiffo, Jonas
Agossou, Vidéhouénou K.
Anagbla, Toussain
Zannou, Marcel
Houngbé, Fabien
Sanni, Ambaliou
author_sort Capo-chichi, Callinice D.
collection PubMed
description BACKGROUND: High risk oncologic Human Papilloma Virus (HPV) is one of the leading causes of cervical cancer worldwide. We investigated HPV genotypes among women living or not with Human Immuno-deficiency Virus (HIV) in two major hospitals in the south of the republic of BENIN in the city of Cotonou. Our objective is to investigate the association of high risk-HPV to cervical dysplasia among women under stringent anti-retroviral (ARV) treatment and in controls without HIV. METHODS: The investigation was carried out within 1 year period in two groups of adult women: one group with HIV1 infection and under ARV therapy in the National University Hospital (CNHU-HKM) designated as CH group (n = 86); and one control group without HIV infection and attending the hospital Mènontin for routine gynecologic checkup and designated as ME group (n = 86). Cells derived from cervical uterine smears (CUS) were used for this investigation. The samples in ME group were selected to have similar lamin A/C profile with CH group. HPV genotypes were assessed by polymerase chain reaction (PCR) while lamin A/C expression profile was assessed by western blotting to corroborate the risk of cervical dysplasia. RESULTS: HPV56 is dominant in CH group while HPV66 is dominant in ME group. 31 % of women in CH group are infected with HPV compared to 23 % in ME group. Quadruple and quintuple HPV infections are more observed among CH group but not in ME group making HPV counts of 43 in CH group and 27 in ME group. Cervical dysplasia are present in 5 % (4/86) of women in CH group and in 1 % (1/86) of women in ME group at the time of CUS collection. The adjustment of the risk to develop cervical cancer in the future related to HPV infection and the total loss of lamin A/C is not significantly different in both groups. CONCLUSION: Women living with HIV are more sensitive to multiple HPV infection but not all HPV infections generated cervical dysplasia. The effectiveness of antiretroviral therapy in CH group may reduce significantly the frequency of cervical dysplasia.
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spelling pubmed-49837862016-08-16 Diversity of high risk human papilloma viruses in women treated with antiretroviral and in healthy controls and discordance with cervical dysplasia in the South of Benin Capo-chichi, Callinice D. Aguida, Blanche Chabi, Nicodème W. Acapko-Ezin, Jocelyn Sossah-Hiffo, Jonas Agossou, Vidéhouénou K. Anagbla, Toussain Zannou, Marcel Houngbé, Fabien Sanni, Ambaliou Infect Agent Cancer Research Article BACKGROUND: High risk oncologic Human Papilloma Virus (HPV) is one of the leading causes of cervical cancer worldwide. We investigated HPV genotypes among women living or not with Human Immuno-deficiency Virus (HIV) in two major hospitals in the south of the republic of BENIN in the city of Cotonou. Our objective is to investigate the association of high risk-HPV to cervical dysplasia among women under stringent anti-retroviral (ARV) treatment and in controls without HIV. METHODS: The investigation was carried out within 1 year period in two groups of adult women: one group with HIV1 infection and under ARV therapy in the National University Hospital (CNHU-HKM) designated as CH group (n = 86); and one control group without HIV infection and attending the hospital Mènontin for routine gynecologic checkup and designated as ME group (n = 86). Cells derived from cervical uterine smears (CUS) were used for this investigation. The samples in ME group were selected to have similar lamin A/C profile with CH group. HPV genotypes were assessed by polymerase chain reaction (PCR) while lamin A/C expression profile was assessed by western blotting to corroborate the risk of cervical dysplasia. RESULTS: HPV56 is dominant in CH group while HPV66 is dominant in ME group. 31 % of women in CH group are infected with HPV compared to 23 % in ME group. Quadruple and quintuple HPV infections are more observed among CH group but not in ME group making HPV counts of 43 in CH group and 27 in ME group. Cervical dysplasia are present in 5 % (4/86) of women in CH group and in 1 % (1/86) of women in ME group at the time of CUS collection. The adjustment of the risk to develop cervical cancer in the future related to HPV infection and the total loss of lamin A/C is not significantly different in both groups. CONCLUSION: Women living with HIV are more sensitive to multiple HPV infection but not all HPV infections generated cervical dysplasia. The effectiveness of antiretroviral therapy in CH group may reduce significantly the frequency of cervical dysplasia. BioMed Central 2016-08-15 /pmc/articles/PMC4983786/ /pubmed/27528886 http://dx.doi.org/10.1186/s13027-016-0090-9 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Capo-chichi, Callinice D.
Aguida, Blanche
Chabi, Nicodème W.
Acapko-Ezin, Jocelyn
Sossah-Hiffo, Jonas
Agossou, Vidéhouénou K.
Anagbla, Toussain
Zannou, Marcel
Houngbé, Fabien
Sanni, Ambaliou
Diversity of high risk human papilloma viruses in women treated with antiretroviral and in healthy controls and discordance with cervical dysplasia in the South of Benin
title Diversity of high risk human papilloma viruses in women treated with antiretroviral and in healthy controls and discordance with cervical dysplasia in the South of Benin
title_full Diversity of high risk human papilloma viruses in women treated with antiretroviral and in healthy controls and discordance with cervical dysplasia in the South of Benin
title_fullStr Diversity of high risk human papilloma viruses in women treated with antiretroviral and in healthy controls and discordance with cervical dysplasia in the South of Benin
title_full_unstemmed Diversity of high risk human papilloma viruses in women treated with antiretroviral and in healthy controls and discordance with cervical dysplasia in the South of Benin
title_short Diversity of high risk human papilloma viruses in women treated with antiretroviral and in healthy controls and discordance with cervical dysplasia in the South of Benin
title_sort diversity of high risk human papilloma viruses in women treated with antiretroviral and in healthy controls and discordance with cervical dysplasia in the south of benin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4983786/
https://www.ncbi.nlm.nih.gov/pubmed/27528886
http://dx.doi.org/10.1186/s13027-016-0090-9
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