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Propolis, A Hope for the Future in Treating Resistant Periodontal Pathogens

Introduction: Periodontitis is one of the most common causes of tooth loss worldwide. Recently, special attention has been paid to natural medication for its treatment. For this purpose, propolis (bee glue) activity has also been investigated. Its antibacterial properties are mainly attributed to fl...

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Autores principales: Shabbir, Ambreen, Rashid, Maryam, Tipu, Hamid N
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cureus 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4985230/
https://www.ncbi.nlm.nih.gov/pubmed/27563508
http://dx.doi.org/10.7759/cureus.682
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author Shabbir, Ambreen
Rashid, Maryam
Tipu, Hamid N
author_facet Shabbir, Ambreen
Rashid, Maryam
Tipu, Hamid N
author_sort Shabbir, Ambreen
collection PubMed
description Introduction: Periodontitis is one of the most common causes of tooth loss worldwide. Recently, special attention has been paid to natural medication for its treatment. For this purpose, propolis (bee glue) activity has also been investigated. Its antibacterial properties are mainly attributed to flavonones pinocembrin, flavonols galangin and to the caffeic acid phenethyl ester. This study is aimed at evaluating the antimicrobial effects of propolis from Pakistan on 35 clinical isolates of pigmented anaerobic periodontal pathogens. Methods: This study was conducted in the Microbiology department, University of Health Sciences, Lahore, Pakistan. Pathogens included were Porphyromonas asaccharolytica (n=9), Porphyromonas gingivalis (n=13), Prevotella intermedia (n=9), Prevotella melaninogenica (n=4). Minimum inhibitory concentration (MIC) to three antibiotics was obtained by E-test method. All strains were sensitive to amoxicillin plus clavulanic acid and metronidazole, but 100% of P asaccharolytica and P melaninogenica strains displayed intermediate resistance to tetracycline while 69.2% P gingivalis and 100% P intermedia strains exhibited complete resistance to tetracycline. Screening for antibacterial activity of propolis extract was done by agar well diffusion assay, and all strains were found sensitive to ethanolic extract of propolis. Results: MIC was obtained by agar incorporation technique with values ranging from 0.064 to 0.512 mg/ml. It was also noticed that percentage yield of ethanolic extract of propolis prepared from ultrasonic extraction method was higher compared to extract obtained with maceration. Conclusion: These results indicate that propolis from this region has potent antimicrobial activity against pigmented anaerobic periodontal pathogens. Taking into consideration the increasing resistance in anaerobic bacteria, this effective antimicrobial activity of propolis gives hope in the treatment of oral cavity diseases.
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spelling pubmed-49852302016-08-25 Propolis, A Hope for the Future in Treating Resistant Periodontal Pathogens Shabbir, Ambreen Rashid, Maryam Tipu, Hamid N Cureus Pathology Introduction: Periodontitis is one of the most common causes of tooth loss worldwide. Recently, special attention has been paid to natural medication for its treatment. For this purpose, propolis (bee glue) activity has also been investigated. Its antibacterial properties are mainly attributed to flavonones pinocembrin, flavonols galangin and to the caffeic acid phenethyl ester. This study is aimed at evaluating the antimicrobial effects of propolis from Pakistan on 35 clinical isolates of pigmented anaerobic periodontal pathogens. Methods: This study was conducted in the Microbiology department, University of Health Sciences, Lahore, Pakistan. Pathogens included were Porphyromonas asaccharolytica (n=9), Porphyromonas gingivalis (n=13), Prevotella intermedia (n=9), Prevotella melaninogenica (n=4). Minimum inhibitory concentration (MIC) to three antibiotics was obtained by E-test method. All strains were sensitive to amoxicillin plus clavulanic acid and metronidazole, but 100% of P asaccharolytica and P melaninogenica strains displayed intermediate resistance to tetracycline while 69.2% P gingivalis and 100% P intermedia strains exhibited complete resistance to tetracycline. Screening for antibacterial activity of propolis extract was done by agar well diffusion assay, and all strains were found sensitive to ethanolic extract of propolis. Results: MIC was obtained by agar incorporation technique with values ranging from 0.064 to 0.512 mg/ml. It was also noticed that percentage yield of ethanolic extract of propolis prepared from ultrasonic extraction method was higher compared to extract obtained with maceration. Conclusion: These results indicate that propolis from this region has potent antimicrobial activity against pigmented anaerobic periodontal pathogens. Taking into consideration the increasing resistance in anaerobic bacteria, this effective antimicrobial activity of propolis gives hope in the treatment of oral cavity diseases. Cureus 2016-07-12 /pmc/articles/PMC4985230/ /pubmed/27563508 http://dx.doi.org/10.7759/cureus.682 Text en Copyright © 2016, Shabbir et al. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Pathology
Shabbir, Ambreen
Rashid, Maryam
Tipu, Hamid N
Propolis, A Hope for the Future in Treating Resistant Periodontal Pathogens
title Propolis, A Hope for the Future in Treating Resistant Periodontal Pathogens
title_full Propolis, A Hope for the Future in Treating Resistant Periodontal Pathogens
title_fullStr Propolis, A Hope for the Future in Treating Resistant Periodontal Pathogens
title_full_unstemmed Propolis, A Hope for the Future in Treating Resistant Periodontal Pathogens
title_short Propolis, A Hope for the Future in Treating Resistant Periodontal Pathogens
title_sort propolis, a hope for the future in treating resistant periodontal pathogens
topic Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4985230/
https://www.ncbi.nlm.nih.gov/pubmed/27563508
http://dx.doi.org/10.7759/cureus.682
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