Cargando…

The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes

Chromatin undergoes dramatic condensation and decondensation as cells transition between the different phases of the cell cycle. The organization of chromatin in chromosomes is still one of the key challenges in structural biology. Fluorescence lifetime imaging (FLIM), a technique which utilizes a f...

Descripción completa

Detalles Bibliográficos
Autores principales:	Estandarte, Ana Katrina, Botchway, Stanley, Lynch, Christophe, Yusuf, Mohammed, Robinson, Ian
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4985626/ https://www.ncbi.nlm.nih.gov/pubmed/27526631 http://dx.doi.org/10.1038/srep31417

Ejemplares similares

Combining Multicolor FISH with Fluorescence Lifetime Imaging for Chromosomal Identification and Chromosomal Sub Structure Investigation
por: Bhartiya, Archana, et al.
Publicado: (2021)

Three-dimensional positioning and structure of chromosomes in a human prophase nucleus
por: Chen, Bo, et al.
Publicado: (2017)

Single molecule localization microscopy of the distribution of chromatin using Hoechst and DAPI fluorescent probes
por: Szczurek, Aleksander T, et al.
Publicado: (2014)

Exploration of DAPI analogues: Synthesis, antitrypanosomal activity, DNA binding and fluorescence properties
por: Farahat, Abdelbasset A., et al.
Publicado: (2017)

Spatially Resolved Quantification of Chromatin Condensation through Differential Local Rheology in Cell Nuclei Fluorescence Lifetime Imaging
por: Spagnol, Stephen T., et al.
Publicado: (2016)

Scanning electron microscope studies of human metaphase chromosomes
por: Shemilt, L. A., et al.
Publicado: (2014)

Fluorescent naphthalimide boronates as theranostics: structural investigations, confocal fluorescence and multiphoton fluorescence lifetime imaging microscopy in living cells
por: Green, Megan J., et al.
Publicado: (2023)

Karyotyping Human Chromosomes by Optical and X-Ray Ptychography Methods
por: Shemilt, Laura, et al.
Publicado: (2015)

Correction: Spatially Resolved Quantification of Chromatin Condensation through Differential Local Rheology in Cell Nuclei Fluorescence Lifetime Imaging
por: Spagnol, Stephen T., et al.
Publicado: (2016)

X-ray Ptychography Imaging of Human Chromosomes After Low-dose Irradiation
por: Bhartiya, Archana, et al.
Publicado: (2021)

Correction to: X-ray ptychography imaging of human chromosomes after low-dose irradiation
por: Bhartiya, Archana, et al.
Publicado: (2021)

Microfluidic Cell Cycle Analysis of Spread Cells by DAPI Staining †
por: Sun, Jing, et al.
Publicado: (2017)

Cycles of protein condensation and discharge in nuclear organelles studied by fluorescence lifetime imaging
por: Pliss, Artem, et al.
Publicado: (2019)

Automatic stage identification of Drosophila egg chamber based on DAPI images
por: Jia, Dongyu, et al.
Publicado: (2016)

Mitotic chromosome condensation resets chromatin to safeguard transcriptional homeostasis during interphase
por: Ramos-Alonso, Lucía, et al.
Publicado: (2023)

The use of fluorescence in situ hybridisation combined with premature chromosome condensation for the identification of chromosome damage.
por: Evans, J. W., et al.
Publicado: (1991)

Heterogeneity in tumor chromatin-doxorubicin binding revealed by in vivo fluorescence lifetime imaging confocal endomicroscopy
por: Sparks, Hugh, et al.
Publicado: (2018)

Axial contraction and short-range compaction of chromatin synergistically promote mitotic chromosome condensation
por: Kruitwagen, Tom, et al.
Publicado: (2015)

Fluorescent protein lifetimes report densities and phases of nuclear condensates during embryonic stem-cell differentiation
por: Joron, Khalil, et al.
Publicado: (2023)

Streamlined histone-based fluorescence lifetime imaging microscopy (FLIM) for studying chromatin organisation
por: Sherrard, Alice, et al.
Publicado: (2018)

Rapid Enrichment and Isolation of Polyphosphate-Accumulating Organisms Through 4’6-Diamidino-2-Phenylindole (DAPI) Staining With Fluorescence-Activated Cell Sorting (FACS)
por: Terashima, Mia, et al.
Publicado: (2020)

(99m)Tc-Labeled HYNIC-DAPI Causes Plasmid DNA Damage with High Efficiency
por: Kotzerke, Joerg, et al.
Publicado: (2014)

Self-Assembling Systems Based on Pillar[5]arenes and Surfactants for Encapsulation of Diagnostic Dye DAPI
por: Nazarova, Anastasia, et al.
Publicado: (2021)

DNA-DAPI Interaction-Based Method for Cell Proliferation Rate Evaluation in 3D Structures
por: Šimoliūnas, Egidijus, et al.
Publicado: (2021)

Efficacy of Endodontic Disinfection Protocols in an E. faecalis Biofilm Model—Using DAPI Staining and SEM
por: Dede, Maria, et al.
Publicado: (2023)

Structurally divergent histone H1 variants in chromosomes containing highly condensed interphase chromatin
Publicado: (1994)

Fluorescence and phosphorescence lifetime imaging reveals a significant cell nuclear viscosity and refractive index changes upon DNA damage
por: Clancy, Ellen, et al.
Publicado: (2023)

Dynamic monitoring of CD45-/CD31+/DAPI+ circulating endothelial cells aneuploid for chromosome 8 during neoadjuvant chemotherapy in locally advanced breast cancer
por: Ma, Ge, et al.
Publicado: (2020)

mTOR direct interactions with Rheb-GTPase and raptor: sub-cellular localization using fluorescence lifetime imaging
por: Yadav, Rahul B, et al.
Publicado: (2013)

Transcription, chromatin condensation, and gene migration
por: McNally, James G.
Publicado: (2009)

Scaffold Hopping in Discovery of HIV-1 Non-Nucleoside Reverse Transcriptase Inhibitors: From CH(CN)-DABOs to CH(CN)-DAPYs
por: Li, Ting-Ting, et al.
Publicado: (2020)

Visualization of chromosome condensation in plants with large chromosomes
por: Kuznetsova, Maria A., et al.
Publicado: (2017)

Condensed mitotic chromatin is accessible to transcription factors and chromatin structural proteins
por: Chen, Danyang, et al.
Publicado: (2005)

Molecular characterization of constitutive heterochromatin in three species of Trypoxylon (Hymenoptera: Crabronidae: Trypoxylini) by CMA3/DAPI staining
por: Menezes, Rodolpho Santos Telles, et al.
Publicado: (2011)

Cannabidiol Modulates Mitochondrial Redox and Dynamics in MCF7 Cancer Cells: A Study Using Fluorescence Lifetime Imaging Microscopy of NAD(P)H
por: Mould, Rhys Richard, et al.
Publicado: (2021)

Chromatin de-condensation by switching substrate elasticity
por: Rabineau, Morgane, et al.
Publicado: (2018)

Epigenetic condensates regulate chromatin activity and tumorigenesis
por: Shi, Bi, et al.
Publicado: (2021)

Chromatin and chromosomes
por: Gilbert, David M., et al.
Publicado: (2011)

Chromatin condensation fluctuations rather than steady-state predict chromatin accessibility
por: Audugé, Nicolas, et al.
Publicado: (2019)

Application of Fluorescence Lifetime Imaging Microscopy of DNA Binding Dyes to Assess Radiation-Induced Chromatin Compaction Changes
por: Abdollahi, Elham, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_932marmlst6nh2h3rc29uctotg