Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum

The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe a robust nano liquid chromatography-mass spectrometry (nanoLC-MS) platform fo...

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Autores principales: Brandtzaeg, Ole Kristian, Johnsen, Elin, Roberg-Larsen, Hanne, Seip, Knut Fredrik, MacLean, Evan L., Gesquiere, Laurence R., Leknes, Siri, Lundanes, Elsa, Wilson, Steven Ray
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4985690/
https://www.ncbi.nlm.nih.gov/pubmed/27528413
http://dx.doi.org/10.1038/srep31693
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author Brandtzaeg, Ole Kristian
Johnsen, Elin
Roberg-Larsen, Hanne
Seip, Knut Fredrik
MacLean, Evan L.
Gesquiere, Laurence R.
Leknes, Siri
Lundanes, Elsa
Wilson, Steven Ray
author_facet Brandtzaeg, Ole Kristian
Johnsen, Elin
Roberg-Larsen, Hanne
Seip, Knut Fredrik
MacLean, Evan L.
Gesquiere, Laurence R.
Leknes, Siri
Lundanes, Elsa
Wilson, Steven Ray
author_sort Brandtzaeg, Ole Kristian
collection PubMed
description The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe a robust nano liquid chromatography-mass spectrometry (nanoLC-MS) platform for measuring the total amount of OT in human plasma/serum. OT binds strongly to plasma proteins, but a reduction/alkylation (R/A) procedure breaks this bond, enabling ample detection of total OT. The method (R/A + robust nanoLC-MS) was used to determine total OT plasma/serum levels to startlingly high concentrations (high pg/mL-ng/mL). Similar results were obtained when combining R/A and ELISA. Compared to measuring free OT, measuring total OT can have advantages in e.g. biomarker studies.
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spelling pubmed-49856902016-08-22 Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum Brandtzaeg, Ole Kristian Johnsen, Elin Roberg-Larsen, Hanne Seip, Knut Fredrik MacLean, Evan L. Gesquiere, Laurence R. Leknes, Siri Lundanes, Elsa Wilson, Steven Ray Sci Rep Article The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe a robust nano liquid chromatography-mass spectrometry (nanoLC-MS) platform for measuring the total amount of OT in human plasma/serum. OT binds strongly to plasma proteins, but a reduction/alkylation (R/A) procedure breaks this bond, enabling ample detection of total OT. The method (R/A + robust nanoLC-MS) was used to determine total OT plasma/serum levels to startlingly high concentrations (high pg/mL-ng/mL). Similar results were obtained when combining R/A and ELISA. Compared to measuring free OT, measuring total OT can have advantages in e.g. biomarker studies. Nature Publishing Group 2016-08-16 /pmc/articles/PMC4985690/ /pubmed/27528413 http://dx.doi.org/10.1038/srep31693 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Brandtzaeg, Ole Kristian
Johnsen, Elin
Roberg-Larsen, Hanne
Seip, Knut Fredrik
MacLean, Evan L.
Gesquiere, Laurence R.
Leknes, Siri
Lundanes, Elsa
Wilson, Steven Ray
Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum
title Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum
title_full Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum
title_fullStr Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum
title_full_unstemmed Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum
title_short Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum
title_sort proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4985690/
https://www.ncbi.nlm.nih.gov/pubmed/27528413
http://dx.doi.org/10.1038/srep31693
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