Cargando…
Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage
Mouse fetal intestinal progenitors lining the epithelium prior to villogenesis grow as spheroids when cultured ex vivo and express the transmembrane glycoprotein Trop2 as a marker. Here, we report the characterization of Trop2-expressing cells from fetal pre-glandular stomach, growing as immortal un...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4986166/ https://www.ncbi.nlm.nih.gov/pubmed/26989172 http://dx.doi.org/10.1242/dev.131490 |
_version_ | 1782448158071062528 |
---|---|
author | Fernandez Vallone, Valeria Leprovots, Morgane Strollo, Sandra Vasile, Gabriela Lefort, Anne Libert, Frederick Vassart, Gilbert Garcia, Marie-Isabelle |
author_facet | Fernandez Vallone, Valeria Leprovots, Morgane Strollo, Sandra Vasile, Gabriela Lefort, Anne Libert, Frederick Vassart, Gilbert Garcia, Marie-Isabelle |
author_sort | Fernandez Vallone, Valeria |
collection | PubMed |
description | Mouse fetal intestinal progenitors lining the epithelium prior to villogenesis grow as spheroids when cultured ex vivo and express the transmembrane glycoprotein Trop2 as a marker. Here, we report the characterization of Trop2-expressing cells from fetal pre-glandular stomach, growing as immortal undifferentiated spheroids, and their relationship with gastric development and regeneration. Trop2(+) cells generating gastric spheroids differed from adult glandular Lgr5(+) stem cells, but appeared highly related to fetal intestinal spheroids. Although they shared a common spheroid signature, intestinal and gastric fetal spheroid-generating cells expressed organ-specific transcription factors and were committed to intestinal and glandular gastric differentiation, respectively. Trop2 expression was transient during glandular stomach development, being lost at the onset of gland formation, whereas it persisted in the squamous forestomach. Undetectable under homeostasis, Trop2 was strongly re-expressed in glands after acute Lgr5(+) stem cell ablation or following indomethacin-induced injury. These highly proliferative reactive adult Trop2(+) cells exhibited a transcriptome displaying similarity with that of gastric embryonic Trop2(+) cells, suggesting that epithelium regeneration in adult stomach glands involves the partial re-expression of a fetal genetic program. |
format | Online Article Text |
id | pubmed-4986166 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-49861662016-09-06 Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage Fernandez Vallone, Valeria Leprovots, Morgane Strollo, Sandra Vasile, Gabriela Lefort, Anne Libert, Frederick Vassart, Gilbert Garcia, Marie-Isabelle Development Stem Cells and Regeneration Mouse fetal intestinal progenitors lining the epithelium prior to villogenesis grow as spheroids when cultured ex vivo and express the transmembrane glycoprotein Trop2 as a marker. Here, we report the characterization of Trop2-expressing cells from fetal pre-glandular stomach, growing as immortal undifferentiated spheroids, and their relationship with gastric development and regeneration. Trop2(+) cells generating gastric spheroids differed from adult glandular Lgr5(+) stem cells, but appeared highly related to fetal intestinal spheroids. Although they shared a common spheroid signature, intestinal and gastric fetal spheroid-generating cells expressed organ-specific transcription factors and were committed to intestinal and glandular gastric differentiation, respectively. Trop2 expression was transient during glandular stomach development, being lost at the onset of gland formation, whereas it persisted in the squamous forestomach. Undetectable under homeostasis, Trop2 was strongly re-expressed in glands after acute Lgr5(+) stem cell ablation or following indomethacin-induced injury. These highly proliferative reactive adult Trop2(+) cells exhibited a transcriptome displaying similarity with that of gastric embryonic Trop2(+) cells, suggesting that epithelium regeneration in adult stomach glands involves the partial re-expression of a fetal genetic program. The Company of Biologists Ltd 2016-05-01 /pmc/articles/PMC4986166/ /pubmed/26989172 http://dx.doi.org/10.1242/dev.131490 Text en © 2016. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Stem Cells and Regeneration Fernandez Vallone, Valeria Leprovots, Morgane Strollo, Sandra Vasile, Gabriela Lefort, Anne Libert, Frederick Vassart, Gilbert Garcia, Marie-Isabelle Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage |
title | Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage |
title_full | Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage |
title_fullStr | Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage |
title_full_unstemmed | Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage |
title_short | Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage |
title_sort | trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage |
topic | Stem Cells and Regeneration |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4986166/ https://www.ncbi.nlm.nih.gov/pubmed/26989172 http://dx.doi.org/10.1242/dev.131490 |
work_keys_str_mv | AT fernandezvallonevaleria trop2markstransientgastricfetalepitheliumandadultregeneratingcellsafterepithelialdamage AT leprovotsmorgane trop2markstransientgastricfetalepitheliumandadultregeneratingcellsafterepithelialdamage AT strollosandra trop2markstransientgastricfetalepitheliumandadultregeneratingcellsafterepithelialdamage AT vasilegabriela trop2markstransientgastricfetalepitheliumandadultregeneratingcellsafterepithelialdamage AT lefortanne trop2markstransientgastricfetalepitheliumandadultregeneratingcellsafterepithelialdamage AT libertfrederick trop2markstransientgastricfetalepitheliumandadultregeneratingcellsafterepithelialdamage AT vassartgilbert trop2markstransientgastricfetalepitheliumandadultregeneratingcellsafterepithelialdamage AT garciamarieisabelle trop2markstransientgastricfetalepitheliumandadultregeneratingcellsafterepithelialdamage |