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Bioconjugation and stabilisation of biomolecules in biosensors

Suitable bioconjugation strategies and stabilisation of biomolecules on electrodes is essential for the development of novel and commercially viable biosensors. In the present review, the functional groups that comprise the selectable targets for practical bioconjugation methods are discussed. We fo...

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Detalles Bibliográficos
Autores principales: Liébana, Susana, Drago, Guido A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Limited 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4986470/
https://www.ncbi.nlm.nih.gov/pubmed/27365036
http://dx.doi.org/10.1042/EBC20150007
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author Liébana, Susana
Drago, Guido A.
author_facet Liébana, Susana
Drago, Guido A.
author_sort Liébana, Susana
collection PubMed
description Suitable bioconjugation strategies and stabilisation of biomolecules on electrodes is essential for the development of novel and commercially viable biosensors. In the present review, the functional groups that comprise the selectable targets for practical bioconjugation methods are discussed. We focus on describing the most common immobilisation techniques used in biosensor construction, which are classified into irreversible and reversible methods. Concerning the stability of proteins, the two main types of stability may be defined as (i) storage or shelf stability, and (ii) operational stability. Both types of stability are explained, as well as the introduction of an electrophoretic technique for predicting protein–polymer interactions. In addition, solution and dry stabilisation as well as stabilisation using the covalent immobilisation of proteins are discussed including possible factors that influence stability. Finally, the integration of nanomaterials, such as magnetic particles, with protein immobilisation is discussed in relation to protein stability studies.
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spelling pubmed-49864702016-08-29 Bioconjugation and stabilisation of biomolecules in biosensors Liébana, Susana Drago, Guido A. Essays Biochem Article Suitable bioconjugation strategies and stabilisation of biomolecules on electrodes is essential for the development of novel and commercially viable biosensors. In the present review, the functional groups that comprise the selectable targets for practical bioconjugation methods are discussed. We focus on describing the most common immobilisation techniques used in biosensor construction, which are classified into irreversible and reversible methods. Concerning the stability of proteins, the two main types of stability may be defined as (i) storage or shelf stability, and (ii) operational stability. Both types of stability are explained, as well as the introduction of an electrophoretic technique for predicting protein–polymer interactions. In addition, solution and dry stabilisation as well as stabilisation using the covalent immobilisation of proteins are discussed including possible factors that influence stability. Finally, the integration of nanomaterials, such as magnetic particles, with protein immobilisation is discussed in relation to protein stability studies. Portland Press Limited 2016-06-30 2016-06-30 /pmc/articles/PMC4986470/ /pubmed/27365036 http://dx.doi.org/10.1042/EBC20150007 Text en © 2016 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
spellingShingle Article
Liébana, Susana
Drago, Guido A.
Bioconjugation and stabilisation of biomolecules in biosensors
title Bioconjugation and stabilisation of biomolecules in biosensors
title_full Bioconjugation and stabilisation of biomolecules in biosensors
title_fullStr Bioconjugation and stabilisation of biomolecules in biosensors
title_full_unstemmed Bioconjugation and stabilisation of biomolecules in biosensors
title_short Bioconjugation and stabilisation of biomolecules in biosensors
title_sort bioconjugation and stabilisation of biomolecules in biosensors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4986470/
https://www.ncbi.nlm.nih.gov/pubmed/27365036
http://dx.doi.org/10.1042/EBC20150007
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