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Sucrose is an early modulator of the key hormonal mechanisms controlling bud outgrowth in Rosa hybrida

Sugar has only recently been identified as a key player in triggering bud outgrowth, while hormonal control of bud outgrowth is already well established. To get a better understanding of sugar control, the present study investigated how sugar availability modulates the hormonal network during bud ou...

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Autores principales: Barbier, François, Péron, Thomas, Lecerf, Marion, Perez-Garcia, Maria-Dolores, Barrière, Quentin, Rolčík, Jakub, Boutet-Mercey, Stéphanie, Citerne, Sylvie, Lemoine, Remi, Porcheron, Benoît, Roman, Hanaé, Leduc, Nathalie, Le Gourrierec, José, Bertheloot, Jessica, Sakr, Soulaiman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4986866/
https://www.ncbi.nlm.nih.gov/pubmed/25873679
http://dx.doi.org/10.1093/jxb/erv047
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author Barbier, François
Péron, Thomas
Lecerf, Marion
Perez-Garcia, Maria-Dolores
Barrière, Quentin
Rolčík, Jakub
Boutet-Mercey, Stéphanie
Citerne, Sylvie
Lemoine, Remi
Porcheron, Benoît
Roman, Hanaé
Leduc, Nathalie
Le Gourrierec, José
Bertheloot, Jessica
Sakr, Soulaiman
author_facet Barbier, François
Péron, Thomas
Lecerf, Marion
Perez-Garcia, Maria-Dolores
Barrière, Quentin
Rolčík, Jakub
Boutet-Mercey, Stéphanie
Citerne, Sylvie
Lemoine, Remi
Porcheron, Benoît
Roman, Hanaé
Leduc, Nathalie
Le Gourrierec, José
Bertheloot, Jessica
Sakr, Soulaiman
author_sort Barbier, François
collection PubMed
description Sugar has only recently been identified as a key player in triggering bud outgrowth, while hormonal control of bud outgrowth is already well established. To get a better understanding of sugar control, the present study investigated how sugar availability modulates the hormonal network during bud outgrowth in Rosa hybrida. Other plant models, for which mutants are available, were used when necessary. Buds were grown in vitro to manipulate available sugars. The temporal patterns of the hormonal regulatory network were assessed in parallel with bud outgrowth dynamics. Sucrose determined bud entrance into sustained growth in a concentration-dependent manner. Sustained growth was accompanied by sustained auxin production in buds, and sustained auxin export in a DR5::GUS-expressing pea line. Several events occurred ahead of sucrose-stimulated bud outgrowth. Sucrose upregulated early auxin synthesis genes (RhTAR1, RhYUC1) and the auxin efflux carrier gene RhPIN1, and promoted PIN1 abundance at the plasma membrane in a pPIN1::PIN1-GFP-expressing tomato line. Sucrose downregulated both RwMAX2, involved in the strigolactone-transduction pathway, and RhBRC1, a repressor of branching, at an early stage. The presence of sucrose also increased stem cytokinin content, but sucrose-promoted bud outgrowth was not related to that pathway. In these processes, several non-metabolizable sucrose analogues induced sustained bud outgrowth in R. hybrida, Pisum sativum, and Arabidopsis thaliana, suggesting that sucrose was involved in a signalling pathway. In conclusion, we identified potential hormonal candidates for bud outgrowth control by sugar. They are central to future investigations aimed at disentangling the processes that underlie regulation of bud outgrowth by sugar.
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spelling pubmed-49868662016-08-22 Sucrose is an early modulator of the key hormonal mechanisms controlling bud outgrowth in Rosa hybrida Barbier, François Péron, Thomas Lecerf, Marion Perez-Garcia, Maria-Dolores Barrière, Quentin Rolčík, Jakub Boutet-Mercey, Stéphanie Citerne, Sylvie Lemoine, Remi Porcheron, Benoît Roman, Hanaé Leduc, Nathalie Le Gourrierec, José Bertheloot, Jessica Sakr, Soulaiman J Exp Bot Research Paper Sugar has only recently been identified as a key player in triggering bud outgrowth, while hormonal control of bud outgrowth is already well established. To get a better understanding of sugar control, the present study investigated how sugar availability modulates the hormonal network during bud outgrowth in Rosa hybrida. Other plant models, for which mutants are available, were used when necessary. Buds were grown in vitro to manipulate available sugars. The temporal patterns of the hormonal regulatory network were assessed in parallel with bud outgrowth dynamics. Sucrose determined bud entrance into sustained growth in a concentration-dependent manner. Sustained growth was accompanied by sustained auxin production in buds, and sustained auxin export in a DR5::GUS-expressing pea line. Several events occurred ahead of sucrose-stimulated bud outgrowth. Sucrose upregulated early auxin synthesis genes (RhTAR1, RhYUC1) and the auxin efflux carrier gene RhPIN1, and promoted PIN1 abundance at the plasma membrane in a pPIN1::PIN1-GFP-expressing tomato line. Sucrose downregulated both RwMAX2, involved in the strigolactone-transduction pathway, and RhBRC1, a repressor of branching, at an early stage. The presence of sucrose also increased stem cytokinin content, but sucrose-promoted bud outgrowth was not related to that pathway. In these processes, several non-metabolizable sucrose analogues induced sustained bud outgrowth in R. hybrida, Pisum sativum, and Arabidopsis thaliana, suggesting that sucrose was involved in a signalling pathway. In conclusion, we identified potential hormonal candidates for bud outgrowth control by sugar. They are central to future investigations aimed at disentangling the processes that underlie regulation of bud outgrowth by sugar. Oxford University Press 2015-05 2015-04-13 /pmc/articles/PMC4986866/ /pubmed/25873679 http://dx.doi.org/10.1093/jxb/erv047 Text en © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Barbier, François
Péron, Thomas
Lecerf, Marion
Perez-Garcia, Maria-Dolores
Barrière, Quentin
Rolčík, Jakub
Boutet-Mercey, Stéphanie
Citerne, Sylvie
Lemoine, Remi
Porcheron, Benoît
Roman, Hanaé
Leduc, Nathalie
Le Gourrierec, José
Bertheloot, Jessica
Sakr, Soulaiman
Sucrose is an early modulator of the key hormonal mechanisms controlling bud outgrowth in Rosa hybrida
title Sucrose is an early modulator of the key hormonal mechanisms controlling bud outgrowth in Rosa hybrida
title_full Sucrose is an early modulator of the key hormonal mechanisms controlling bud outgrowth in Rosa hybrida
title_fullStr Sucrose is an early modulator of the key hormonal mechanisms controlling bud outgrowth in Rosa hybrida
title_full_unstemmed Sucrose is an early modulator of the key hormonal mechanisms controlling bud outgrowth in Rosa hybrida
title_short Sucrose is an early modulator of the key hormonal mechanisms controlling bud outgrowth in Rosa hybrida
title_sort sucrose is an early modulator of the key hormonal mechanisms controlling bud outgrowth in rosa hybrida
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4986866/
https://www.ncbi.nlm.nih.gov/pubmed/25873679
http://dx.doi.org/10.1093/jxb/erv047
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