The fission yeast MTREC and EJC orthologs ensure the maturation of meiotic transcripts during meiosis

Meiosis is a highly regulated process by which genetic information is transmitted through sexual reproduction. It encompasses unique mechanisms that do not occur in vegetative cells, producing a distinct, well-regulated meiotic transcriptome. During vegetative growth, many meiotic genes are constitu...

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Autores principales: Marayati, Bahjat Fadi, Hoskins, Victoria, Boger, Robert W., Tucker, James F., Fishman, Emily S., Bray, Andrew S., Zhang, Ke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4986891/
https://www.ncbi.nlm.nih.gov/pubmed/27365210
http://dx.doi.org/10.1261/rna.055608.115
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author Marayati, Bahjat Fadi
Hoskins, Victoria
Boger, Robert W.
Tucker, James F.
Fishman, Emily S.
Bray, Andrew S.
Zhang, Ke
author_facet Marayati, Bahjat Fadi
Hoskins, Victoria
Boger, Robert W.
Tucker, James F.
Fishman, Emily S.
Bray, Andrew S.
Zhang, Ke
author_sort Marayati, Bahjat Fadi
collection PubMed
description Meiosis is a highly regulated process by which genetic information is transmitted through sexual reproduction. It encompasses unique mechanisms that do not occur in vegetative cells, producing a distinct, well-regulated meiotic transcriptome. During vegetative growth, many meiotic genes are constitutively transcribed, but most of the resulting mRNAs are rapidly eliminated by the Mmi1-MTREC (Mtl1-Red1 core) complex. While Mmi1-MTREC targets premature meiotic RNAs for degradation by the nuclear 3′–5′ exoribonuclease exosome during mitotic growth, its role in meiotic gene expression during meiosis is not known. Here, we report that Red5, an essential MTREC component, interacts with pFal1, an ortholog of eukaryotic translation initiation factor eIF4aIII in the fission yeast Schizosaccharomyces pombe. In mammals, together with MAGO (Mnh1), Rnps1, and Y14, elF4AIII (pFal1) forms the core of the exon junction complex (EJC), which is essential for transcriptional surveillance and localization of mature mRNAs. In fission yeast, two EJC orthologs, pFal1 and Mnh1, are functionally connected with MTREC, specifically in the process of meiotic gene expression during meiosis. Although pFal1 interacts with Mnh1, Y14, and Rnps1, its association with Mnh1 is not disrupted upon loss of Y14 or Rnps1. Mutations of Red1, Red5, pFal1, or Mnh1 produce severe meiotic defects; the abundance of meiotic transcripts during meiosis decreases; and mRNA maturation processes such as splicing are impaired. Since studying meiosis in mammalian germline cells is difficult, our findings in fission yeast may help to define the general mechanisms involved in accurate meiotic gene expression in higher eukaryotes.
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spelling pubmed-49868912016-09-01 The fission yeast MTREC and EJC orthologs ensure the maturation of meiotic transcripts during meiosis Marayati, Bahjat Fadi Hoskins, Victoria Boger, Robert W. Tucker, James F. Fishman, Emily S. Bray, Andrew S. Zhang, Ke RNA Article Meiosis is a highly regulated process by which genetic information is transmitted through sexual reproduction. It encompasses unique mechanisms that do not occur in vegetative cells, producing a distinct, well-regulated meiotic transcriptome. During vegetative growth, many meiotic genes are constitutively transcribed, but most of the resulting mRNAs are rapidly eliminated by the Mmi1-MTREC (Mtl1-Red1 core) complex. While Mmi1-MTREC targets premature meiotic RNAs for degradation by the nuclear 3′–5′ exoribonuclease exosome during mitotic growth, its role in meiotic gene expression during meiosis is not known. Here, we report that Red5, an essential MTREC component, interacts with pFal1, an ortholog of eukaryotic translation initiation factor eIF4aIII in the fission yeast Schizosaccharomyces pombe. In mammals, together with MAGO (Mnh1), Rnps1, and Y14, elF4AIII (pFal1) forms the core of the exon junction complex (EJC), which is essential for transcriptional surveillance and localization of mature mRNAs. In fission yeast, two EJC orthologs, pFal1 and Mnh1, are functionally connected with MTREC, specifically in the process of meiotic gene expression during meiosis. Although pFal1 interacts with Mnh1, Y14, and Rnps1, its association with Mnh1 is not disrupted upon loss of Y14 or Rnps1. Mutations of Red1, Red5, pFal1, or Mnh1 produce severe meiotic defects; the abundance of meiotic transcripts during meiosis decreases; and mRNA maturation processes such as splicing are impaired. Since studying meiosis in mammalian germline cells is difficult, our findings in fission yeast may help to define the general mechanisms involved in accurate meiotic gene expression in higher eukaryotes. Cold Spring Harbor Laboratory Press 2016-09 /pmc/articles/PMC4986891/ /pubmed/27365210 http://dx.doi.org/10.1261/rna.055608.115 Text en © 2016 Marayati et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by/4.0/ This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Marayati, Bahjat Fadi
Hoskins, Victoria
Boger, Robert W.
Tucker, James F.
Fishman, Emily S.
Bray, Andrew S.
Zhang, Ke
The fission yeast MTREC and EJC orthologs ensure the maturation of meiotic transcripts during meiosis
title The fission yeast MTREC and EJC orthologs ensure the maturation of meiotic transcripts during meiosis
title_full The fission yeast MTREC and EJC orthologs ensure the maturation of meiotic transcripts during meiosis
title_fullStr The fission yeast MTREC and EJC orthologs ensure the maturation of meiotic transcripts during meiosis
title_full_unstemmed The fission yeast MTREC and EJC orthologs ensure the maturation of meiotic transcripts during meiosis
title_short The fission yeast MTREC and EJC orthologs ensure the maturation of meiotic transcripts during meiosis
title_sort fission yeast mtrec and ejc orthologs ensure the maturation of meiotic transcripts during meiosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4986891/
https://www.ncbi.nlm.nih.gov/pubmed/27365210
http://dx.doi.org/10.1261/rna.055608.115
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