Cargando…

Microbiota in Breast Milk of Chinese Lactating Mothers

The microbiota of breast milk from Chinese lactating mothers at different stages of lactation was examined in the framework of a Maternal Infant Nutrition Growth (MING) study investigating the dietary habits and breast milk composition in Chinese urban mothers. We used microbiota profiling based on...

Descripción completa

Detalles Bibliográficos
Autores principales: Sakwinska, Olga, Moine, Déborah, Delley, Michèle, Combremont, Séverine, Rezzonico, Enea, Descombes, Patrick, Vinyes-Pares, Gerard, Zhang, Yumei, Wang, Peiyu, Thakkar, Sagar K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4987007/
https://www.ncbi.nlm.nih.gov/pubmed/27529821
http://dx.doi.org/10.1371/journal.pone.0160856
_version_ 1782448254546345984
author Sakwinska, Olga
Moine, Déborah
Delley, Michèle
Combremont, Séverine
Rezzonico, Enea
Descombes, Patrick
Vinyes-Pares, Gerard
Zhang, Yumei
Wang, Peiyu
Thakkar, Sagar K.
author_facet Sakwinska, Olga
Moine, Déborah
Delley, Michèle
Combremont, Séverine
Rezzonico, Enea
Descombes, Patrick
Vinyes-Pares, Gerard
Zhang, Yumei
Wang, Peiyu
Thakkar, Sagar K.
author_sort Sakwinska, Olga
collection PubMed
description The microbiota of breast milk from Chinese lactating mothers at different stages of lactation was examined in the framework of a Maternal Infant Nutrition Growth (MING) study investigating the dietary habits and breast milk composition in Chinese urban mothers. We used microbiota profiling based on the sequencing of fragments of 16S rRNA gene and specific qPCR for bifidobacteria, lactobacilli and total bacteria to study microbiota of the entire breast milk collected using standard protocol without aseptic cleansing (n = 60), and the microbiota of the milk collected aseptically (n = 30). We have also investigated the impact of the delivery mode and the stage of lactation on the microbiota composition. The microbiota of breast milk was dominated by streptococci and staphylococci for both collection protocols and, in the case of standard collection protocol, Acinetobacter sp. While the predominance of streptococci and staphylococci was consistently reported previously for other populations, the abundance of Acinetobacter sp. was reported only once before in a study where milk collection was done without aseptic cleansing of the breast and rejection of foremilk. Higher bacterial counts were found in the milk collected using standard protocol. Bifidobacteria and lactobacilli were present in few samples with low abundance. We observed no effect of the stage of lactation or the delivery mode on microbiota composition. Methodological and geographical differences likely explain the variability in microbiota composition reported to date.
format Online
Article
Text
id pubmed-4987007
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-49870072016-08-29 Microbiota in Breast Milk of Chinese Lactating Mothers Sakwinska, Olga Moine, Déborah Delley, Michèle Combremont, Séverine Rezzonico, Enea Descombes, Patrick Vinyes-Pares, Gerard Zhang, Yumei Wang, Peiyu Thakkar, Sagar K. PLoS One Research Article The microbiota of breast milk from Chinese lactating mothers at different stages of lactation was examined in the framework of a Maternal Infant Nutrition Growth (MING) study investigating the dietary habits and breast milk composition in Chinese urban mothers. We used microbiota profiling based on the sequencing of fragments of 16S rRNA gene and specific qPCR for bifidobacteria, lactobacilli and total bacteria to study microbiota of the entire breast milk collected using standard protocol without aseptic cleansing (n = 60), and the microbiota of the milk collected aseptically (n = 30). We have also investigated the impact of the delivery mode and the stage of lactation on the microbiota composition. The microbiota of breast milk was dominated by streptococci and staphylococci for both collection protocols and, in the case of standard collection protocol, Acinetobacter sp. While the predominance of streptococci and staphylococci was consistently reported previously for other populations, the abundance of Acinetobacter sp. was reported only once before in a study where milk collection was done without aseptic cleansing of the breast and rejection of foremilk. Higher bacterial counts were found in the milk collected using standard protocol. Bifidobacteria and lactobacilli were present in few samples with low abundance. We observed no effect of the stage of lactation or the delivery mode on microbiota composition. Methodological and geographical differences likely explain the variability in microbiota composition reported to date. Public Library of Science 2016-08-16 /pmc/articles/PMC4987007/ /pubmed/27529821 http://dx.doi.org/10.1371/journal.pone.0160856 Text en © 2016 Sakwinska et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sakwinska, Olga
Moine, Déborah
Delley, Michèle
Combremont, Séverine
Rezzonico, Enea
Descombes, Patrick
Vinyes-Pares, Gerard
Zhang, Yumei
Wang, Peiyu
Thakkar, Sagar K.
Microbiota in Breast Milk of Chinese Lactating Mothers
title Microbiota in Breast Milk of Chinese Lactating Mothers
title_full Microbiota in Breast Milk of Chinese Lactating Mothers
title_fullStr Microbiota in Breast Milk of Chinese Lactating Mothers
title_full_unstemmed Microbiota in Breast Milk of Chinese Lactating Mothers
title_short Microbiota in Breast Milk of Chinese Lactating Mothers
title_sort microbiota in breast milk of chinese lactating mothers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4987007/
https://www.ncbi.nlm.nih.gov/pubmed/27529821
http://dx.doi.org/10.1371/journal.pone.0160856
work_keys_str_mv AT sakwinskaolga microbiotainbreastmilkofchineselactatingmothers
AT moinedeborah microbiotainbreastmilkofchineselactatingmothers
AT delleymichele microbiotainbreastmilkofchineselactatingmothers
AT combremontseverine microbiotainbreastmilkofchineselactatingmothers
AT rezzonicoenea microbiotainbreastmilkofchineselactatingmothers
AT descombespatrick microbiotainbreastmilkofchineselactatingmothers
AT vinyesparesgerard microbiotainbreastmilkofchineselactatingmothers
AT zhangyumei microbiotainbreastmilkofchineselactatingmothers
AT wangpeiyu microbiotainbreastmilkofchineselactatingmothers
AT thakkarsagark microbiotainbreastmilkofchineselactatingmothers