Measurement of Histone Methylation Dynamics by One-Carbon Metabolic Isotope Labeling and High-energy Collisional Dissociation Methylation Signature Ion Detection

Accumulating evidence suggests that cellular metabolites and nutrition levels control epigenetic modifications, including histone methylation. However, it is not currently possible to measure the metabolic control of histone methylation. Here we report a novel detection method to monitor methyl tran...

Descripción completa

Detalles Bibliográficos
Autores principales: Tang, Hui, Tian, Bing, Brasier, Allan R., Sowers, Lawrence C., Zhang, Kangling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4987619/
https://www.ncbi.nlm.nih.gov/pubmed/27530234
http://dx.doi.org/10.1038/srep31537
_version_ 1782448329239560192
author Tang, Hui
Tian, Bing
Brasier, Allan R.
Sowers, Lawrence C.
Zhang, Kangling
author_facet Tang, Hui
Tian, Bing
Brasier, Allan R.
Sowers, Lawrence C.
Zhang, Kangling
author_sort Tang, Hui
collection PubMed
description Accumulating evidence suggests that cellular metabolites and nutrition levels control epigenetic modifications, including histone methylation. However, it is not currently possible to measure the metabolic control of histone methylation. Here we report a novel detection method to monitor methyl transfer from serine to histones through the one-carbon metabolic pathway, using stable-isotope labeling and detection of lysine methylation signature ions generated in high-energy-dissociation (HCD) tandem mass spectrometry. This method is a long-needed tool to study the metabolic control of histone methylation.
format Online
Article
Text
id pubmed-4987619
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-49876192016-08-30 Measurement of Histone Methylation Dynamics by One-Carbon Metabolic Isotope Labeling and High-energy Collisional Dissociation Methylation Signature Ion Detection Tang, Hui Tian, Bing Brasier, Allan R. Sowers, Lawrence C. Zhang, Kangling Sci Rep Article Accumulating evidence suggests that cellular metabolites and nutrition levels control epigenetic modifications, including histone methylation. However, it is not currently possible to measure the metabolic control of histone methylation. Here we report a novel detection method to monitor methyl transfer from serine to histones through the one-carbon metabolic pathway, using stable-isotope labeling and detection of lysine methylation signature ions generated in high-energy-dissociation (HCD) tandem mass spectrometry. This method is a long-needed tool to study the metabolic control of histone methylation. Nature Publishing Group 2016-08-17 /pmc/articles/PMC4987619/ /pubmed/27530234 http://dx.doi.org/10.1038/srep31537 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Tang, Hui
Tian, Bing
Brasier, Allan R.
Sowers, Lawrence C.
Zhang, Kangling
Measurement of Histone Methylation Dynamics by One-Carbon Metabolic Isotope Labeling and High-energy Collisional Dissociation Methylation Signature Ion Detection
title Measurement of Histone Methylation Dynamics by One-Carbon Metabolic Isotope Labeling and High-energy Collisional Dissociation Methylation Signature Ion Detection
title_full Measurement of Histone Methylation Dynamics by One-Carbon Metabolic Isotope Labeling and High-energy Collisional Dissociation Methylation Signature Ion Detection
title_fullStr Measurement of Histone Methylation Dynamics by One-Carbon Metabolic Isotope Labeling and High-energy Collisional Dissociation Methylation Signature Ion Detection
title_full_unstemmed Measurement of Histone Methylation Dynamics by One-Carbon Metabolic Isotope Labeling and High-energy Collisional Dissociation Methylation Signature Ion Detection
title_short Measurement of Histone Methylation Dynamics by One-Carbon Metabolic Isotope Labeling and High-energy Collisional Dissociation Methylation Signature Ion Detection
title_sort measurement of histone methylation dynamics by one-carbon metabolic isotope labeling and high-energy collisional dissociation methylation signature ion detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4987619/
https://www.ncbi.nlm.nih.gov/pubmed/27530234
http://dx.doi.org/10.1038/srep31537
work_keys_str_mv AT tanghui measurementofhistonemethylationdynamicsbyonecarbonmetabolicisotopelabelingandhighenergycollisionaldissociationmethylationsignatureiondetection
AT tianbing measurementofhistonemethylationdynamicsbyonecarbonmetabolicisotopelabelingandhighenergycollisionaldissociationmethylationsignatureiondetection
AT brasierallanr measurementofhistonemethylationdynamicsbyonecarbonmetabolicisotopelabelingandhighenergycollisionaldissociationmethylationsignatureiondetection
AT sowerslawrencec measurementofhistonemethylationdynamicsbyonecarbonmetabolicisotopelabelingandhighenergycollisionaldissociationmethylationsignatureiondetection
AT zhangkangling measurementofhistonemethylationdynamicsbyonecarbonmetabolicisotopelabelingandhighenergycollisionaldissociationmethylationsignatureiondetection

Ejemplares similares