Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP

Predictable, clean genetic modification (GM) in livestock is important for reliable phenotyping and biosafety. Here we reported the generation of isozygous, functional myostatin (MSTN) knockout cloned pigs free of selectable marker gene (SMG) by CRISPR/Cas9 and Cre/LoxP. CRISPR/Cas9-mediated homolog...

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Detalles Bibliográficos
Autores principales: Bi, Yanzhen, Hua, Zaidong, Liu, Ximei, Hua, Wenjun, Ren, Hongyan, Xiao, Hongwei, Zhang, Liping, Li, Li, Wang, Zhirui, Laible, Götz, Wang, Yan, Dong, Faming, Zheng, Xinmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4987667/
https://www.ncbi.nlm.nih.gov/pubmed/27530319
http://dx.doi.org/10.1038/srep31729

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