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CRISPR-PCS: a powerful new approach to inducing multiple chromosome splitting in Saccharomyces cerevisiae

PCR-mediated chromosome splitting (PCS) was developed in the yeast Saccharomyces cerevisiae. It is based on homologous recombination and enables division of a chromosome at any point to form two derived and functional chromosomes. However, because of low homologous recombination activity, PCS is lim...

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Detalles Bibliográficos
Autores principales:	Sasano, Yu, Nagasawa, Koki, Kaboli, Saeed, Sugiyama, Minetaka, Harashima, Satoshi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4987674/ https://www.ncbi.nlm.nih.gov/pubmed/27530680 http://dx.doi.org/10.1038/srep30278

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